Determination of Pitavastatin from Human Plasma Using High Performance Liquid Chromatography with Fluorescence Detection

Ojha, Ashwini; Guttikar, Swati; Vayeda, Chintan; Padh, Harish

doi:10.1016/s1872-2059(07)60025-x

Cited by 8 publications

(4 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There are only a limited number of chromatographic methods for quantifying pitavastatin concentrations in human plasma, with the majority relying on mass detection [2][3][4][5]. One method involves the use of UV [6], and two others involve the use of fluorescence detection [7,8]. Concerning the latter, Ojha et al used 1 mL of human plasma, liquid-liquid extraction, and an injection volume of 85 µL, while Zhou and colleagues used 100 µL of plasma, protein precipitation, and a 50 µL injection volume but no internal standard.…”

Section: Introductionmentioning

confidence: 99%

Precision Meets Affordability: A Highly Sensitive HPLC-FLD Technique for Accurate Pitavastatin Quantification in Human Plasma

Basso,

Fortuna,

Vitorino

et al. 2023

Csac 2023

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

Precision Meets Affordability: A Highly Sensitive HPLC-FLD Technique for Accurate Pitavastatin Quantification in Human Plasma

Basso,

Fortuna,

Vitorino

et al. 2023

Csac 2023

View full text Add to dashboard Cite

show abstract

“…[4][5][6][7] PTV was approved by USFDA in 2009. A literature survey revealed that pitavastatin can be estimated by spectrophotometry, [8] high-performance thin-layer chromatography (HPTLC), [9,10] and liquid chromatography (LC) [11][12][13] in bulk and pharmaceutical formulation. The LC=MS=MS method has been reported for the estimation of pitavastatin in human plasma.…”

Section: Introductionmentioning

confidence: 99%

Kinetic Determinations of Pitavastatin Calcium by Stability Indicating HPTLC Method

Akabari¹,

Shah²,

Shah³

et al. 2014

Journal of Liquid Chromatography & Related Technologies

View full text Add to dashboard Cite

A simple, sensitive, precise, and stability-indicating high-performance thin-layer chromatographic method has been developed and validated for the quantitative determination of pitavastatin calcium (PTV) in a pharmaceutical dosage form in the presence of its degradation products. The stability of PTV was investigated under different stress conditions, including hydrolytic, oxidative, photolytic, and thermal, as recommended by the International Conference on Harmonization guidelines. PTV was separated on aluminum-backed silica gel 60 F 254 high-performance thin-layer chromatography (HPTLC) plates as a stationary phase with ethyl acetate-methanol-toluene-glacial acetic acid (4:1:5:0.1, v=v=v=v) as a mobile phase. Regression analysis data for the calibration plots were indicative of good linear relationships between responses and concentration over the range 25-150 ng per band. The method was validated for linearity, precision, accuracy, robustness, specificity, and sensitivity. The drug was subjected to stress degradation and peaks of all the degradation products were well resolved from that of the pure drug, with significantly different R f value, which indicates the specificity and stability-indicating properties of the method. The kinetic determination was evaluated in acid conditions. The acid degradation of PTV showed an apparent first-order kinetics and rate constants were found to be 0.00202 mg=mL=min in 0.1 N HCl at 75 C.

show abstract

“…The calibration curves of NK-104 and NK-104 lactone were linear over the ranges 0.5 to 100 ng/ml for human plasma samples and 0.5 to 500 ng/ml for dog plasma, respectively [7]. [8] reported an analytical method for the determination of the drug from human plasma making use of the fluorescent property of the drug. Plasma is extracted from the drug using ethyl acetate and then analyzed by reversed-phase high performance liquid chromatography (HPLC) with fluorescence detection using C 18 HPLC column with gradient flow of mobile phase (0.01 mol/L monobasic potassium phosphate (pH 3.20) acetonitrile, 63:37, v/v) [8].…”

Section: Introductionmentioning

confidence: 99%

“…[8] reported an analytical method for the determination of the drug from human plasma making use of the fluorescent property of the drug. Plasma is extracted from the drug using ethyl acetate and then analyzed by reversed-phase high performance liquid chromatography (HPLC) with fluorescence detection using C 18 HPLC column with gradient flow of mobile phase (0.01 mol/L monobasic potassium phosphate (pH 3.20) acetonitrile, 63:37, v/v) [8]. HPTLC determination of pitavastatin calcium in tablet dosage form were studied with the mobile phase of ethyl acetatemethanol-ammonia-1 drop formic acid (7:2:0.8) [9].…”

Section: Introductionmentioning

confidence: 99%

HPLC Determination of Pitavastatin Calcium in Pharmaceutical Dosage Forms

Satheeshkumar¹,

Nisha²,

Nirmal³

et al. 2011

Pharm Anal Acta

View full text Add to dashboard Cite

A simple, sensitive, reliable and rapid reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed and validated for the determination of pitavastatin calcium using paracetamol as internal standard. The chromatographic system consisted of Shimadzu LC-10ATVP Pump, SPD-M10 AVP with PDA detector. Separation was achieved on the phenomenex C 18 (250 x 4.60), 5 μ particle size column in isocratic mode at room temperature. The sample was introduced through an injector valve with a 20 μl, sample loop. 0.5% Acetic acid: Acetonitrile 35:65 (%, v/v), was used as mobile phase with flow rate of 1 ml/min. UV detection was performed at 245 nm. A calibration graph was plotted which showed a linearity range between 1-5 μg/ml with the correlation coefficient of 0.9986. The LOD was 5 ng/ ml, while the LOQ was 20 ng/ml. Validation studies revealed the method is specific, rapid, reliable, and reproducible. To study the validity of the method, recovery studies and repeatability studies were carried out using the same optimum conditions. The system suitability studies were also calculated which includes column efficiency, resolution, capacity factor and peak asymmetrical factor. Therefore the proposed method is reliable, rapid, precise and selective so may be used for the quantitative analysis of pitavastatin calcium.

show abstract

Determination of Pitavastatin from Human Plasma Using High Performance Liquid Chromatography with Fluorescence Detection

Cited by 8 publications

References 4 publications

Precision Meets Affordability: A Highly Sensitive HPLC-FLD Technique for Accurate Pitavastatin Quantification in Human Plasma

Precision Meets Affordability: A Highly Sensitive HPLC-FLD Technique for Accurate Pitavastatin Quantification in Human Plasma

Kinetic Determinations of Pitavastatin Calcium by Stability Indicating HPTLC Method

HPLC Determination of Pitavastatin Calcium in Pharmaceutical Dosage Forms

Contact Info

Product

Resources

About