Determination of plant parasitic nematodes associated with chickpea in Turkey

Abstract

A survey of plant parasitic nematodes associated with chickpea was conducted in the chickpea growing areas of Turkey including 37 districts in 17 provinces during spring and summer of 2014-2016. A total of 211 soil and root samples were collected. Nematodes were extracted from soil by different extraction methods to ensure all kinds of nematode groups. Nematodes were identified using morphological and morphometric features. In addition, Pratylenchus spp. Filipjev, 1936 were determined using species-specific primers. Ditylenchus dipsaci (Kühn, 1857), Pratylenchus neglectus (Rensch, 1924) and Pratylenchus thornei Sher & Allen, 1953 were the most common of the plant parasitic nematodes associated with chickpea in the areas surveyed.

Investigation of the development of root lesion nematodes, Pratylenchus spp. (Tylenchida: Pratylenchidae) in three chickpea cultivars

“…Nematodes used in this study were collected during April and May 2014 to 2016 from a chickpea field (37º08'29" N 38º46'30" E) in Harran District, Şanlıurfa p8province, Turkey (Behmand et al, 2019). The climate is arid to semiarid in that area.…”

“…The root lesion nematodes including P. neglectus, P. penetrans and P. thornei, have been commonly determined in fields in Turkey (Şahin et al, 2008;Söğüt & Devran, 2011;Akyazi et al, 2018). Also, Behmand et al (2019) showed that these nematodes have been found widely distributed in 82% of the chickpea fields in many regions in Turkey.…”

“…Rebois & Huettel (1986) indicated that there was a significant difference between the number of nematodes in the roots and agar in soybean and corn; the population density of eggs and individuals in the roots was greatest than nematode population in agar. Pratylenchus thornei Sher & Allen, 1953 (Tylenchida: Pratylenchidae) was found on 24 plant species that reproduced well on 97 chickpea lines in Syria and Turkey (Greco et al, 1988;Gomez-Barcina et al, 1996;Behmand et al, 2019). There are different factors that can affected on root lesion [P. thornei and Pratylenchus penetrans (Cobb, 1917) (Tylenchida: Pratylenchidae)] egg hatching.…”

Development of methodology for resistance screening of chickpea genotypes collected in Turkey to the root lesion nematode, Pratylenchus thornei Sher & Allen, 1953 (Tylenchida: Pratylenchidae)

“…The root lesion nematodes, Pratylenchus spp. [Pratylenchus thornei Sher &Allen, 1953 andPratylenchus neglectus Rensch, 1924 (Tylenchida, Pratylenchidae)], are the most important constraint to legume production and have a wide distribution in in many regions in Turkey (82% of chickpea fields) and affect many agricultural crops around the world (Tanha Maafi et al, 2009;Behmand et al, 2019). Root lesion nematodes in chickpea need to be surveyed periodically and conducted risk analysis, based on its economic importance.…”

“…Pratylenchus thornei is one of the most important plant parasitic nematodes causing yield losses of up to 40% in cereals and legumes in dryland cropping areas of southeastern Australia (Thompson et al, 1995;Vanstone, 1998). These nematodes have been found widely distributed in a wheat fields in Turkey (Behmand et al, 2019). However, study of resistance screening methods for chickpea is limited in Turkey.…”