Determination of Plant Volatiles Using Solid Phase Microextraction GC–MS

Bramer, Scott Van; Goodrich, Katherine R.

doi:10.1021/ed5006807

Cited by 14 publications

(21 citation statements)

References 18 publications

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“…In previous studies [ 21 ], VOCs were collected from vines individually enclosed in bags using fibres coated with divinylbenzene (DVB), carboxen (CAR), and polydimethylsiloxane (PDMS). Solid-phase microextraction (SPME) has proved to be a useful tool to collect plant VOCs [ 22 ]. It uses a fused silica fibre coated with a polymeric material to adsorb and pre-concentrate volatile compounds.…”

Section: Resultsmentioning

confidence: 99%

VOCs Are Relevant Biomarkers of Elicitor-Induced Defences in Grapevine

et al. 2021

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Grapevine is susceptible to fungal diseases generally controlled by numerous chemical fungicides. Elicitors of plant defence are a way of reducing the use of these chemicals, but still provide inconsistent efficiency. Easy-to-analyse markers of grapevine responses to elicitors are needed to determine the best conditions for their efficiency and position them in protection strategies. We previously reported that the elicitor sulphated laminarin induced the emission of volatile organic compounds (VOCs) by grapevine leaves. The present study was conducted to characterise and compare VOC emissions in response to other elicitors. Bastid® was first used to test the conditions of VOC collection and analysis. Using SBSE-GC-MS, we detected several VOCs, including the sesquiterpene α-farnesene, in a time-dependent manner. This was correlated with the induction of farnesene synthase gene expression, in parallel with stilbene synthesis (another defence response), and associated to resistance against downy mildew. The other elicitors (Redeli®, Romeo®, Bion®, chitosan, and an oligogalacturonide) induced VOC emission, but with qualitative and quantitative differences. VOC emission thus constitutes a response of grapevine to elicitors of various chemical structures. Therefore, VOC analysis is relevant for studying the impact of environmental factors on grapevine defence responses and optimising the performance of elicitors in vineyards.

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Section: Resultsmentioning

confidence: 99%

VOCs Are Relevant Biomarkers of Elicitor-Induced Defences in Grapevine

et al. 2021

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“…There is an ongoing trend for the use of miniaturized VOC sampling devices because of the simplicity of their application without consumption or interference of important peaks in the GC by organic solvents (Lan et al ., 2020). Since its introduction in the 1990s, solid phase microextraction (SPME) (Figures 1 and 2a) has become a widely popular tool to trap plant VOCs in the lab, the field, or even the classroom (Fung et al ., 2019b; Gharaei et al ., 2020; Van Bramer and Goodrich, 2015). The passive/static sampling technique relies on the adsorption and thermal desorption of compounds from an inert fiber, which is coated with different types of adsorbents of varying polarity and thickness depending on the type of collected compounds and their concentration (Tholl et al ., 2006; Zhu et al ., 2013).…”

Section: Going Simple and Small: Miniaturized Trapping Devicesmentioning

confidence: 99%

Trends and applications in plant volatile sampling and analysis

et al. 2021

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Volatile organic compounds (VOCs) released by plants serve as information and defense chemicals in mutualistic and antagonistic interactions and mitigate effects of abiotic stress. Passive and dynamic sampling techniques combined with gas chromatography-mass spectrometry analysis have become routine tools to measure emissions of VOCs and determine their various functions. More recently, knowledge of the roles of plant VOCs in the aboveground environment has led to the exploration of similar functions in the soil and rhizosphere. Moreover, VOC patterns have been recognized as sensitive and time-dependent markers of biotic and abiotic stress. This focused review addresses these developments by presenting recent progress in VOC sampling and analysis. We show advances in the use of small, inexpensive sampling devices and describe methods to monitor plant VOC emissions in the belowground environment. We further address latest trends in real-time measurements of volatilomes in plant phenotyping and most recent developments of small portable devices and VOC sensors for non-invasive VOC fingerprinting of plant disease. These technologies allow for innovative approaches to study plant VOC biology and application in agriculture.

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“…coated fibers with liquid and solid phases [4][5][6]8]. The SPME process comprises two steps: In the first, the fiber is exposed to the sample or its headspace and the target analytes partition from the sample matrix to the coating.…”

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Magnetic headspace adsorptive extraction of chlorobenzenes prior to thermal desorption gas chromatography-mass spectrometry

Vidal

Ahmadi

Fernández

et al. 2017

Analytica Chimica Acta

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This study presents a new, user-friendly, cost-effective and portable headspace solid-phase extraction technique based on graphene oxide decorated with iron oxide magnetic nanoparticles as sorbent, located on one end of a small neodymium magnet. Hence, the new headspace solid-phase extraction technique has been called Magnetic Headspace Adsorptive Extraction (Mag-HSAE). In order to assess Mag-HSAE technique applicability to model analytes, some chlorobenzenes were extracted from water samples prior to gas chromatography-mass spectrometry determination. A multivariate approach was employed to optimize the experimental parameters affecting Mag-HSAE. The method was evaluated under optimized extraction conditions (i.e., sample volume, 20 mL; extraction time, 30 min; sorbent amount, 10 mg; stirring speed, 1500 rpm, and ionic strength, non-significant), obtaining a linear response from 0.5 to 100 ng L for 1,3-DCB, 1,4-DCB, 1,2-DCB, 1,3,5-TCB, 1,2,4-TCB and 1,2,3-TCB; from 0.5 to 75 ng L for 1,2,4,5-TeCB, and PeCB; and from 1 to 75 ng L for 1,2,3,4-TeCB. The repeatability of the proposed method was evaluated at 10 ng L and 50 ng L spiking levels, and coefficients of variation ranged between 1.5 and 9.5% (n = 5). Limits of detection values were found between 93 and 301 pg L. Finally, tap, mineral and effluent water were selected as real water samples to assess method applicability. Relative recoveries varied between 86 and 110% showing negligible matrix effects.

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Determination of Plant Volatiles Using Solid Phase Microextraction GC–MS

Cited by 14 publications

References 18 publications

VOCs Are Relevant Biomarkers of Elicitor-Induced Defences in Grapevine

VOCs Are Relevant Biomarkers of Elicitor-Induced Defences in Grapevine

Trends and applications in plant volatile sampling and analysis

Magnetic headspace adsorptive extraction of chlorobenzenes prior to thermal desorption gas chromatography-mass spectrometry

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