Determination of plasma amino acids by gas chromatography

Chemistry, analysis, nutritional value, and toxicology of tryptophan in food. A review

“…Because tryptophan has a strong affinity for serum albumin, difficulties are often encountered in measuring serum-bound and free tryptophan in plasma Labadarios et al, 1986; Gjerde et al, 1987). Additional research is also needed to devise improved methods to differentiate between free and albumin-bound tryptophan in body fluids.…”

Determination of Arginine δ15N Values in Plant and Animal Proteins by Gas Chromatography–Combustion–Isotope Ratio Mass Spectrometry

“…While the GC and gas chromatography−mass spectrometry (GC−MS) analysis of Arg has been reported, these derivatization methods involve the addition of bulky groups with a large number of C atoms, such as heptafluorobutyryl isobutyl ester (HBB) derivatives, 19 N(O,S)-tert-butyldimethylsilyl (TBDMS) derivatives, 20 N-pentafluoropropionyl methyl (PFPM) esters, 21 or even enzymatic conversion of Arg to Orn before derivatization to N(O,S)-isobutoxycarbonyl methyl esters. 22 These derivatization procedures are all problematic for GC−C−IRMS analyses, however.…”

Diamond Paste Based Amperometric Biosensors for the Determination ofl- andd-Pipecolic Acid

“…1) is an important biological marker for the diagnosis of peroxisomal disorders (e.g., cerebrohepatorenal syndrome, neonatal adrenoleukodystrophy, infantile Refsum disease) while D-pipecolic acid (D-PA) ( Fig. Pipecolic acid and its derivatives were determined from biological samples using high performance liquid chromatography (HPLC), [5][6][7][8][9][10][11][12][13][14][15] gas chromatography, [16][17][18][19] liquid chromatography, [4,20] ion-exchange chromatography, [21] and electrophoresis techniques. [1][2][3][4] Many analytical methods have been reported to analyze pipecolic acid using expensive instrumentation and long sample preparation.…”