Determination of plasma and brain concentrations of trazodone and its metabolite, l-m-chlorophenylpiperazine, by gas-liquid chromatography

Plasma Levels of Trazodone: Methodology and Applications

“…Blank plasma samples are consistently free of con taminants in the areas corresponding to reten tion times of trazodone and chlorohaloperidol. The described active metabolite of trazo done, l-m-chlorophenylpiperazine [12], does not interfere with this method. The signifi cance of this metabolite is unclear at this time, however it has been demonstrated by other workers not to achieve appreciable plasma concentrations after single doses in man [12].…”

“…The described active metabolite of trazo done, l-m-chlorophenylpiperazine [12], does not interfere with this method. The signifi cance of this metabolite is unclear at this time, however it has been demonstrated by other workers not to achieve appreciable plasma concentrations after single doses in man [12]. This method and these pilot pharmacoki netic data indicate that after a single 50-mg oral dose, trazodone is rapidly absorbed, with a peak plasma level achieved within 30 min.…”

Química forense: perspectivas sobre novos métodos analíticos aplicados à documentoscopia, balística e drogas de abuso

“…No caso de testes colorimétricos como o reativo de Marquis, onde a adição de algumas gotas de formaldeído em meio ácido produz uma cor preta para resultados positivos na presença de MDMA, fornecem resultados negativos se apenas derivados da piperazina estiverem presentes no comprimido, onde nenhuma variação de cor será observada. 79 Outras técnicas são usadas para identificação das piperazinas, como cromatografia gasosa acoplada com espectrômetro de massas (GC-MS), 80 cromatografia líquida de alta eficiência acoplada com detector na região do ultravioleta (HPLC-UV) 81 e LC-MS. 82 Entretanto, a grande maioria das metodologias analíticas é considerada complexa, por envolver preparação da amostra, ou pouco específica, como a TLC. Uma forma alternativa, rápida, confiável e reprodutível é a utilização da técnica EASI-MS.…”

A translational, caffeine-induced model of onset insomnia in rats and healthy volunteers

“…Preliminary experiments in our laboratory showed that a dose of 10 mg/kg trazodone produced no change in SOL either alone or in response to caffeine (data not shown); therefore, a 20-mg/kg dose was chosen. Dose times were chosen to ensure that sufficient drug was absorbed at the start of recording to produce the desired effect (Caccia et al 1981;Garrigou-Gadenne et al 1989;Wang and Lau 1998). Exact time of drug administration is referred to in relation to light onset at 1400 hours.…”