Determination of Polyhexamethylene Biguanide Hydrochloride Using a Lactone-Rhodamine B-Based Fluorescence Optode

Abstract

A new determination method for polyhexamethylene biguanide hydrochloride (PHMB) using a lactone-rhodamine B (L-RB) based fluorescence optode has been developed. The optode membrane consists of 2-nitrophenyl octyl ether as a plasticizer, L-RB, and poly (vinyl chloride). The optode responds to tetrakis (4-fluorophenyl) borate, sodium salt (NaTPBF) in the μM range. The fluorescence intensity of the L-RB film for PHMB solution containing 20 μM NaTPBF decreased linearly as the concentration of the PHMB solution increased in the concentration range from 0 to 8.0 μM, which shows that PHMB with a concentration range of 0 to 8.0 μM is determined by the L-RB film optode. The concentration of PHMB in the contact lens detergents by the proposed method was in accord with its nominal concentration.

Poly(hexamethylene biguanide) hydrochloride (PHMB)-based materials: synthesis, modification, properties, determination, and application

“…88 Funaki and coworkers devised a chemical sensor for PHMB determination by using a fluorescence optode based on lactone rhodamine B with good accuracy and sensitivity. 89 Liu et al proposed an analytical method for PHMB determination in compound chemical disinfectants based on micellar electrokinetic chromatography with bi-micelle. 90 This method was capable of discriminating PHMB from other chemicals in commercial samples, thereby avoiding interference in the quantification of PHMB.…”

Biosafety evaluation and quantitative determination of poly(hexamethylene biguanide) (PHMB) coated on cellulosic fabrics by Kubelka–Munk equation

“…Relatively more sophisticated chromatographic approaches such as gas chromatography (GC), gradient gel-permeation chromatography (GPC) and highperformance liquid chromatography (HPLC) are precise methods to quantify cationic finishing agents in fabrics (Lucas et al 2009;Masadome et al 2018). Recently, various novel methods with high sensitivity such as glucose oxidase enzymatic reaction analytical method and Lactone-rhodamine B-based fluorescence optode technique have been introduced to quantify poly(hexamethylene biguanide) hydrochloride (PHMB) in the solution used for contact lens (Funaki et al 2020;Masadome et al 2018;Uematsu et al 2019). However, these analytical methods are primarily suitable for trace analysis of cationic agents in aqueous, such as industrial wastewater and contact lens solution, and are incapable of quantifying cationic surfactants on the textile fabrics.…”

Recent Trends in Pharmaceutical Analytical Chemistry

“…Dr. Masadome and co-workers, in their article “ Determination of Polyhexamethylene Biguanide Hydrochloride Using a Lactone-Rhodamine B-Based Fluorescence Optode ”, reported a new approach for the quantitation of polyhexamethylene biguanide using a lactone–rhodamine B fluorescence optode [ 9 ]. The analyte is a cationic polyelectrolyte which is used for disinfectants in contact lens detergents and sanitizers for swimming pools.…”