Determination of polyphenolic content in beverages using laccase, gold nanoparticles and long wavelength fluorimetry

Andreu-Navarro, A.; Fernández-Romero, J.M.; Gómez‐Hens, A.

doi:10.1016/j.aca.2011.11.049

Cited by 24 publications

(28 citation statements)

References 19 publications

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“…The enzymes utilized in these sensors typically involve polyphenol oxidases (PPO) (e.g., tyrosinase and laccase) [13,16–22] or peroxidases (e.g., HRP). Some limitations of tyrosinase- and laccase-based biosensors include that they can only monitor phenolic compounds with atleast one free ortho -position or free p ara - and meta -position [23].…”

Section: Introductionmentioning

confidence: 99%

Chemically glycosylation improves the stability of an amperometric horseradish peroxidase biosensor

Hernández-Cancel

Suazo-Dávila

Medina-Guzmán

et al. 2015

Analytica Chimica Acta

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We constructed a biosensor by electrodeposition of gold nano-particles (AuNPs) on glassy carbon (GC) and subsequent formation of a 4-mercaptobenzoic acid self-assembled monolayer (SAM). The enzyme horseradish peroxidase (HRP) was then covalently immobilized onto the SAM. Two forms of HRP were employed: non-modified and chemically glycosylated with lactose. Circular dichroism (CD) spectra showed that chemical glycosylation did neither change the tertiary structure of HRP nor the heme environment. The highest sensitivity of the biosensor to hydroquinone was obtained for the biosensor with HRP-lactose 1 (414 nA μM−1 ) compared to 378 nA μM−1 for the one employing non-modified HRP. The chemically glycosylated form of the enzyme catalyzed the reduction of hydroquinone more rapidly than the native form of the enzyme. The sensor employing lactose-modified HRP also had a lower limit of detection (74 μM) than the HRP biosensor (83 μM). However, most importantly, chemically glycosylation improved the long-term stability of the biosensor, which retained 60% of its activity over a four-month storage period compared to only 10% for HRP. These results highlight improvements by an innovative stabilization method when compared to previously reported enzyme-based biosensors.

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Section: Introductionmentioning

confidence: 99%

Chemically glycosylation improves the stability of an amperometric horseradish peroxidase biosensor

Hernández-Cancel

Suazo-Dávila

Medina-Guzmán

et al. 2015

Analytica Chimica Acta

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“…Another peak was observed between 400 and 600°C that was attributed to the dehydrochlorination of FeCl 2 . 42 For the FeCl 3 (Fig. 3(c)), a peak was observed before 200°C, with a mass loss of approximately 40%.…”

Section: Resultsmentioning

confidence: 92%

Development of modified graphite electrodes with nanoparticles and their application in the quantification of sucrose

Santos

Ribeiro

Oliveira

et al. 2022

J of Chemical Tech & Biotech

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BACKGROUND Developing fast and reliable methods with little sample preparation has become increasingly important for process monitoring and control. Electrochemical methods stand out for their low cost and instrumental simplicity. Furthermore, electrodes with nanoparticles can improve the method's sensitivity and specificity. This study developed carbon paste electrodes modified with nanoparticles of copper and iron. In addition, cyclic voltammetry was performed using a low‐cost potentiostat designed for a Raspberry Pi single‐board computer. As a proof of concept, the constructed electrodes were tested with ferrocyanide and applied to quantify sucrose, and their performance was compared to that of the glassy carbon electrode. RESULTS The nanoparticles were successfully synthesized, as confirmed by morphology and physicochemical characterization. Based on the adjusted R2, the electrode made with the addition of copper oxide nanoparticles has a similar performance to the commercial glassy carbon electrode. All electrodes' root mean square error (RMSE) values were approximately 10−4 mol L−1. The lowest values for the inverse of the analytical sensitivity for sucrose were obtained for the unmodified carbon paste electrode (9.54 × 10−6 mol L−1) and copper oxide nanoparticles electrode (5.30 × 10−6 mol L−1). These electrodes also had the lowest limits of detection (10−5 mol L−1) and, for the limit of quantification, all electrodes had similar values (10−2 mol L−1). CONCLUSION In general, superior performance of carbon paste electrodes was observed concerning the commercial glassy carbon electrodes. Compared to previous studies, the developed electrodes showed good sensitivity and the low cost of the electrochemical system stands out as the main advantage. © 2022 Society of Chemical Industry (SCI).

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“…Andreu-Navarro et al [55] proposed another interesting optical sensing approach for the determination of polyphenols, such as catechol, resorcinol, hydroquinone, pyrogallol, hydroxyhydroquinone, phloroglucinol, and gallic acid. This biosensor is based on the inhibition of the green indocyanine fluorescence in the presence of laccase and positively charged gold nanoparticles caused by polyphenols.…”

Section: Laccase-based Biosensorsmentioning

confidence: 99%

Advanced Optical Sensing of Phenolic Compounds for Environmental Applications

Delfino

Diano

Lepore

2021

Sensors

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Phenolic compounds are particularly dangerous due to their ability to remain in the environment for a long period of time and their toxic effects. They enter in the environment in different ways, such as waste from paper manufacturing, agriculture (pesticides, insecticides, herbicides), pharmaceuticals, the petrochemical industry, and coal processing. Conventional methods for phenolic compounds detection present some disadvantages, such as cumbersome sample preparation, complex and time-consuming procedures, and need of expensive equipment. Therefore, there is a very large interest in developing sensors and new sensing schemes for fast and easy-to-use methods for detecting and monitoring the phenolic compound concentration in the environment, with special attention to water. Good analytical properties, reliability, and adaptability are required for the developed sensors. The present paper aims at revising the most generally used optical methods for designing and fabricating biosensors and sensors for phenolic compounds. Some selected examples of the most interesting applications of these techniques are also proposed.

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Determination of polyphenolic content in beverages using laccase, gold nanoparticles and long wavelength fluorimetry

Cited by 24 publications

References 19 publications

Chemically glycosylation improves the stability of an amperometric horseradish peroxidase biosensor

Chemically glycosylation improves the stability of an amperometric horseradish peroxidase biosensor

Development of modified graphite electrodes with nanoparticles and their application in the quantification of sucrose

Advanced Optical Sensing of Phenolic Compounds for Environmental Applications

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