2016
DOI: 10.1002/pca.2656
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Determination of Polyphenolic Phytochemicals using Highly Deshielded –OH 1H‐NMR Signals

Abstract: Introduction Quantitative analysis of plant extracts is a tedious and time consuming process requiring chromatographic techniques and/or the use of specilised hyphenated spectroscopic methods. Thus, investigation of a rapid method capable for the determination of bioactive constituents is of major importance. Objective To employ high resolution 1H‐NMR spectroscopy of the –OH spectral region, as a rapid method for the simultaneous determination and quantification of polyphenolic phytochemicals in crude oregano … Show more

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Cited by 18 publications
(23 citation statements)
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“…LC-NMR is one of the most powerful methods for separation and structural elucidation of unknown compounds in mixtures [ 169 , 170 , 171 , 172 , 173 ]. LC-NMR, thus, represents a potentially interesting complementary technique to LC-MS in analysis of complex biological matrices for the detailed on-line structural analysis of products.…”
Section: Selected Analytical and Structural Studiesmentioning
confidence: 99%
“…LC-NMR is one of the most powerful methods for separation and structural elucidation of unknown compounds in mixtures [ 169 , 170 , 171 , 172 , 173 ]. LC-NMR, thus, represents a potentially interesting complementary technique to LC-MS in analysis of complex biological matrices for the detailed on-line structural analysis of products.…”
Section: Selected Analytical and Structural Studiesmentioning
confidence: 99%
“…This may be attributed to the fact that –OH groups displayed broad signals and variable chemical shifts at room temperature due to intermolecular exchange of the –OH protons with protons of the protic solvents or with protons of the residual H 2 O in aprotic solvents. However, optimization of experimental conditions, such as pH, temperature and NMR solvent may result in obtaining extremely sharp peaks (Δν 1/2 ≤ 2 Hz) which can lead to unequivocal assignment of the OH signals [ 64 , 65 , 66 , 67 , 92 , 93 , 94 ].…”
Section: Hydrogen Atomic Positions Of Intramolecular O–h···o Hydromentioning
confidence: 99%
“…Proton nuclear magnetic resonance ( 1 H‐NMR), however, has proven successful for quality control of diversified products (food, beverages, coffee, tea, gasoline, olive oil, natural products, biodiesel and vegetable oil in diesel‐biodiesel blends, pediatric syrups excipients, amongst others) . Nevertheless, methods involving 1 H‐NMR quantification of essential oils major constituents are still in their infancy.…”
Section: Introductionmentioning
confidence: 99%
“…11 Proton nuclear magnetic resonance ( 1 H-NMR), however, has proven successful for quality control of diversified products (food, beverages, coffee, tea, gasoline, olive oil, natural products, biodiesel and vegetable oil in diesel-biodiesel blends, pediatric syrups excipients, amongst others). 8,[12][13][14][15][16][17] Nevertheless, methods involving 1 H-NMR quantification of essential oils major constituents are still in their infancy. The paucity of studies employing 1 H-NMR, exemplify difficulty in unravelling the complexity of the matrix in essential oils, despite both its versatility and short analyses time.…”
mentioning
confidence: 99%