Determination of Polyphenols in Commercial Extra Virgin Olive Oils from Different Origins (Mediterranean and South American Countries) by Liquid Chromatography–Electrospray Time-of-Flight Mass Spectrometry

Gilbert-López, Bienvenida; Valencia-Reyes, Zanhy L.; Yufra-Picardo, Velia M.; García-Reyes, Juan F.; Ramos-Martos, Natividad; Molina‐Díaz, Antonio

doi:10.1007/s12161-014-9825-7

Cited by 28 publications

(24 citation statements)

References 23 publications

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“…In addition, HTY and TY were found in higher concentration compared with other compounds, behavior previously reported for other authors. 30 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 …”

Section: Samplesmentioning

confidence: 99%

Matrix solid-phase dispersion: a simple and fast technique for the determination of phenolic compounds in olive oil by liquid chromatography

2014

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A methodology was developed using matrix solid phase dispersion, together with liquid chromatography with multiple wavelength detector for the determination of twenty phenolic compounds in olive oil samples. Under optimized conditions, the analytes were extracted using 0.5 g of olive oil, 1.0 g of Florisil as sorbent and 1 mL methanol:water (80:20 acidified with formic acid 0.5% (v/v)) as eluting solvent. The proposed methodology provided detection and quantification limits of individual compounds in the ranges of 0.02 -0.75 and 0.08 -2.50 mg kg -1 , respectively. The RSDs resulting from the analysis of 6 replicates of 0.5 g of sample pool containing 2.5 mg kg -1 phenolic compounds were ranged between 2.1% and 14.8%. Considering matrix-matched calibration as quantification technique, the average recoveries ranged from 74.8% to 95.0%, with relative standard deviations between 1.5% and 9.3%. The developed methodology was applied for the determination of phenolic compounds in nine olive oils produced in Argentina, identifying seventeen analytes at concentrations above detectable levels.

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Section: Samplesmentioning

confidence: 99%

Matrix solid-phase dispersion: a simple and fast technique for the determination of phenolic compounds in olive oil by liquid chromatography

2014

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“…Different analytical methods have been developed to determine phenolic compounds, using different ways of extraction, separation and quantification techniques . The most critical point is represented by the extraction, because the results of the entire methodology depend on it .…”

Section: Introductionmentioning

confidence: 99%

“…However, a derivatization step is necessary when GC is used , whereas the pitfall of CE was the low concentration sensitivity . On the contrary, promising results in terms of analytical speed and cost reduction were obtained by using HPLC with UV , fluorescence or MS detectors . Further progress was reached using the more recent UHPLC technique.…”

Section: Introductionmentioning

confidence: 99%

Determination of eight polyphenols and pantothenic acid in extra‐virgin olive oil samples by a simple, fast, high‐throughput and sensitive ultra high performance liquid chromatography with tandem mass spectrometry method

Gosetti

Bolfi

Manfredi

et al. 2015

J of Separation Science

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A new ultra high performance liquid chromatography coupled with tandem mass spectrometry method for a fast and sensitive determination of eight polyphenols (hydroxytyrosol, catechin, epicatechin, epigallocatechin gallate, oleuropein, quercetin, rutin, tyrosol) and panthotenic acid in extra-virgin olive oil was developed. The method does not require long sample pre-treatment and presents the lowest limit of detection and limit of quantitation values present in literature. Inter- and intra-day variability, linear dynamic range of the calibration curve, recovery and matrix effect were also determined and investigated. The method was applied to several oil samples of different type and origin. Given its accuracy, precision and rapidity, the method is characterized by an interestingly high throughput, reliability, and sensitivity.

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“…[6] It is therefore important to determine the amounts of these flavones in food. To date, the determination of luteolin and apigenin has been performed by high-performance liquid chromatography (HPLC) [7][8][9] , high-performance thin layer chromatography (HPTLC) [10] , gas chromatography (GC) [11] , fluorimetry [12] , and capillary electrophoresis (CE). [13][14][15][16] HPLC is the most widely used method for separation and quantification of flavonoids from natural sources, mostly coupled with mass spectrometric detection (HPLC-MS).…”

Section: Introductionmentioning

confidence: 99%

“…[13][14][15][16] HPLC is the most widely used method for separation and quantification of flavonoids from natural sources, mostly coupled with mass spectrometric detection (HPLC-MS). [7][8][9] However, CE is a a good alternative to HPLC because of its high resolution, versatility, simplicity, short analysis time, and low consumption of chemicals and samples. Various detection techniques have been coupled to CE.…”

Section: Introductionmentioning

confidence: 99%

Determination of Luteolin and Apigenin in Herbs by Capillary Electrophoresis with Diode Array Detection

Maher

Alzoman

Al‐Shehri

et al. 2015

Instrumentation Science & Technology

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Capillary electrophoresis with a diode array detector was employed for the determination of luteolin and apigenin in plant extracts. The experimental factors affecting the elution of the analytes were carefully optimized. The final analysis was achieved utilizing a fused silica capillary (58 cm effective length, 75 µm ID) and a background electrolyte solution consisting of borax buffer (20 mM, pH 10.0) and methanol (90: 10, v/v) with a 23 kV driving voltage and detection at 210 nm. The method was fully validated as per the guidelines of the International Conference on Harmonization for Analytical Procedures. The relationship between peak area and concentration was linear between 3 and 800 µg/mL for both compounds with detection limits of 1.05 for luteolin and 0.53 µg/mL for apigenin. The method was employed for the determination of the analytes in extracts of thyme and parsley. Both compounds were resolved from interfering peaks that were present in the extracts. Recovery studies were performed by fortifying the extracts with standards and demonstrated good accuracy with recovery values between 97.29 and Downloaded by [Nanyang Technological University] at 18:34 22 August 2015 2 104.88 % for luteolin and 96.89 and 105.18 % for apigenin. Thyme and parsley were shown to contain high concentrations of luteolin and apigenin.

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Determination of Polyphenols in Commercial Extra Virgin Olive Oils from Different Origins (Mediterranean and South American Countries) by Liquid Chromatography–Electrospray Time-of-Flight Mass Spectrometry

Cited by 28 publications

References 23 publications

Matrix solid-phase dispersion: a simple and fast technique for the determination of phenolic compounds in olive oil by liquid chromatography

Matrix solid-phase dispersion: a simple and fast technique for the determination of phenolic compounds in olive oil by liquid chromatography

Determination of eight polyphenols and pantothenic acid in extra‐virgin olive oil samples by a simple, fast, high‐throughput and sensitive ultra high performance liquid chromatography with tandem mass spectrometry method

Determination of Luteolin and Apigenin in Herbs by Capillary Electrophoresis with Diode Array Detection

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