Determination of potassium, sodium, calcium and magnesium in total parenteral nutrition formulations by capillary electrophoresis with contactless conductivity detection

Abstract

A simple method based on capillary electrophoresis with a capacitively coupled contactless conductivity detector (CE-C(4)D) was developed for the determination of potassium, sodium, calcium and magnesium in parenteral nutrition formulations. A hydro-organic mixture, consisting of 100 mM Tris-acetate buffer at pH 4.5 and acetonitrile (80:20, v/v), was selected as the background electrolyte. The applied voltage was 30 kV, and sample injection was performed in hydrodynamic mode. All analyses were carried out in a fused silica capillary with an internal diameter of 50 microm and a total length of 64.5 cm. Under these conditions, complete separation between all cations was achieved in less than 4 min. The CE-C(4)D method was validated, and trueness values between 98.6% and 101.8% were obtained with repeatability and intermediate precision values of 0.4-1.3% and 0.8-1.8%, respectively. Therefore, this method was found to be appropriate for controlling potassium, sodium, calcium and magnesium in parenteral nutrition formulations and successfully applied in daily quality control at the Geneva University Hospitals

Simultaneous Determination of Inorganic Cations by Capillary Ion Chromatography with a Non-suppressed Contactless Conductivity Detector

“…17,18 So, the influence of acetonitrile and methanol on the retention of cations was investigated in the present work. Although acetonitrile was added to the eluent up to 90% (v/v), the selectivity for analyte cations was not improved.…”

Fast analysis of potassium, sodium, calcium, and magnesium cations in total parenteral nutrition formulations with the Wyn-CE Capillary Electrophoresis System coupled with a contactless conductivity detection

“…The resulting electropherogram is shown in Figure 1. The obtained separation exhibited a good separation of the 4 analytes with a resolution superior to 1.2 and an analysis time divided by 2 compared to those obtained by Nussbaumer et al [3]. This gain can be important if the number of TPN to analyze is important.…”

“…The apparition of contactless conductivity detectors offered to users an easy handling of capillary and a simpler buffer conception. Nussbaumer et al already presented a CE method dedicated to the problematic of cation in TPN analyses [3]. This method used a hydro-organic Background Electrolyte (BGE) composed by 100 mM Tris/acetate buffer at pH 4.5 and acetonitrile (80/20, v/v).…”

Determination of Iodide in Seawater by Capillary Ion Chromatography Using Hexadimethrine Bromide Modified C30 Stationary Phases

“…16,17 Thus, the influence of acetonitrile and methanol on the retention of anions was investigated in the present work. Since C30 modified with HDMB is hydrophobic, when an organic substance is added in the eluent, the C30/HDMB modified stationary phase can be solvated or modified with the organic substance added, which could affect the retention of the analyte anions.…”