Determination of Potato Glycoalkaloids Using a Liposome Immunomigration, Liquid-Phase Competition Immunoassay

Glorio‐Paulet, Patricia; Durst, Richard A.

doi:10.1021/jf990349+

Cited by 11 publications

(2 citation statements)

References 31 publications

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“…For the POPC sample, dioxane increases the population of the 156R1 fast component as well as the correlation time of the slow component (MOMD fits not shown), suggesting that dioxane competes with the spin label for the surface of the protein and weakens the interaction between R1 and the protein surface. Interestingly, in DiErPC bilayers, dioxane has no effect at concentrations up to 10% v/v, but solubilizes the liposomes above this threshold, as reported previously ( 36 ). It is not clear why the behavior in DiErPC is different, but this result could indicate that the side chain R1 interacts more strongly with the protein surface in these membranes and is not effectively displaced by dioxane.…”

Section: Resultssupporting

confidence: 83%

Molecular Origin of Electron Paramagnetic Resonance Line Shapes on β-Barrel Membrane Proteins: The Local Solvation Environment Modulates Spin-Label Configuration

et al. 2011

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In the present work, EPR spectroscopy and X-ray crystallography were used to examine the origins of EPR lineshapes from spin labels at the protein-lipid interface on the β-barrel membrane protein BtuB. Two atomic-resolution structures were obtained for the methanethiosulfonate spin label derivatized to cysteines on the membrane-facing surface of BtuB. At one of these sites, position 156, the label side-chain resides in a pocket formed by neighboring side chains; however, it extends from the protein surface and yields a single-component EPR spectrum in the crystal that results primarily from fast rotation about the fourth and fifth bonds linking the spin label to the protein backbone. In lipid bilayers, site 156 yields a multicomponent spectrum resulting from different rotameric states of the labeled side chain. Moreover, changes in the lipid environment, such as variations in bilayer thickness, modulate the EPR spectrum by modulating label rotamer populations. At a second site, position 371, the labeled side chain interacts with a pocket on the protein surface leading to a highly immobilized single component EPR spectrum that is not sensitive to hydrocarbon thickness. This spectrum is similar to that seen at other sites that are deep in the hydrocarbon, such as position 170. This work indicates that the rotameric states of spin labels on exposed hydrocarbon sites are sensitive to the environment at the protein-hydrocarbon interface, and that this environment may modulate weak interactions between the labeled side chain and the protein surface. In the case of BtuB, lipid acyl chain packing is not symmetric around the β-barrel, and EPR spectra from labeled hydrocarbon facing sites in BtuB may reflect this asymmetry. In addition to facilitating the interpretation of EPR spectra from membrane proteins, these results have important implications for the use of long-range distance restraints in protein structure refinement that are obtained from spin labels.

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Section: Resultssupporting

confidence: 83%

Molecular Origin of Electron Paramagnetic Resonance Line Shapes on β-Barrel Membrane Proteins: The Local Solvation Environment Modulates Spin-Label Configuration

et al. 2011

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“…Their results demonstrated the usefulness of immunoliposomes for detecting E. coli O157:H7 in aqueous sample incorporating IMBs and sulforhodamine B. Patricia and Durst (2000) also proposed the application of an LIA to the agricultural field. They developed a liposome immunomigration, liquid-phase competition strip immunoassay for the determination of potato glycoalkaloids.…”

Section: Liposome Nanoparticles As Detection Sensor In Food Sectormentioning

confidence: 93%

Current Demands for Food-Approved Liposome Nanoparticles in Food and Safety Sector

et al. 2017

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Safety of food is a noteworthy issue for consumers and the food industry. A number of complex challenges associated with food engineering and food industries, including quality food production and safety of the food through effective and feasible means can be explained by nanotechnology. However, nanoparticles have unique physicochemical properties compared to normal macroparticles of the same composition and thus could interact with living system in surprising ways to induce toxicity. Further, few toxicological/safety assessments have been performed on nanoparticles, thereby necessitating further research on oral exposure risk prior to their application to food. Liposome nanoparticles are viewed as attractive novel materials by the food and medical industries. For example, nanoencapsulation of bioactive food compounds is an emerging application of nanotechnology. In several food industrial practices, liposome nanoparticles have been utilized to improve flavoring and nutritional properties of food, and they have been examined for their capacity to encapsulate natural metabolites that may help to protect the food from spoilage and degradation. This review focuses on ongoing advancements in the application of liposomes for food and pharma sector.

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Phytochem. Anal.

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Determination of Potato Glycoalkaloids Using a Liposome Immunomigration, Liquid-Phase Competition Immunoassay

Cited by 11 publications

References 31 publications

Molecular Origin of Electron Paramagnetic Resonance Line Shapes on β-Barrel Membrane Proteins: The Local Solvation Environment Modulates Spin-Label Configuration

Molecular Origin of Electron Paramagnetic Resonance Line Shapes on β-Barrel Membrane Proteins: The Local Solvation Environment Modulates Spin-Label Configuration

Current Demands for Food-Approved Liposome Nanoparticles in Food and Safety Sector

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