Determination of Potential Pathogenicity of Staphylococci

Elston, Harry R.; Fitch, Donald M.

doi:10.1093/ajcp/42.4.346

Cited by 12 publications

(5 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A possible enzymatic means for detecting food contamination by Staphylococcus aureus was suggested by the fact that nearly all pathogenic staphylococci produce a unique and remarkably heat-stable nuclease (9,11). Specific detection of this enzyme in food stuffs would provide prima facie evidence of contamination by S. aureus.…”

mentioning

confidence: 99%

Enzymatic Detection of the Growth of Staphylococcus aureus in Foods1

Chesbro¹,

Auborn²

1967

Applied Microbiology

View full text Add to dashboard Cite

A specific method has been developed for the extraction and measurement of staphylococcal nuclease in foods in which Staphylococcus aureus has grown. The method was used to. compare staphylococcal growth with nuclease production in foods under varying conditions of temperature, aerobiosis, and competition from other microorganisms. It was concluded that the nuclease is produced under any conditions that permit growth of S. aureus, and little or no interference with the test was encountered either from mixed, natural populations or from a variety of pure, laboratory cultures. Nuclease and enterotoxin A production were shown to vary in synchrony for the 234 (Casman) strain of S. aureus, and the sensitivity of the enzymatic detection of nuclease was comparable to the sensitivity of serological detection of enterotoxin A. It was found that 15 min at 121 C was required to reduce the nuclease activity in slurries of contaminated ham below the level present in the unheated slurry. The extraordinary heat resistance of the nuclease permits its detection even in foods heated subsequent to the growth of S. aureus. The nuclease analysis requires about 3 hr to complete and requires no unusual equipment or reagents.

show abstract

mentioning

confidence: 99%

Enzymatic Detection of the Growth of Staphylococcus aureus in Foods1

Chesbro¹,

Auborn²

1967

Applied Microbiology

View full text Add to dashboard Cite

show abstract

“…It would be possible for some of our strains to ferment mannitol if the period of observation were extended for seven days, or if they were tested at a mannitol concentration of 1 % in peptone broth (7).…”

Section: Discussionmentioning

confidence: 99%

Comparison of Desoxyribonuclease Activity to Some Other Criteria of <i>Staphylococcus </i>Pathogenicity

Papaevangelou¹,

Papavassiliou²

1967

Pathobiology

View full text Add to dashboard Cite

“…It is highly reasonable to postulate that coagulase might play a role in the pathogenesis of S. aureus through its peculiar biological activity which might reflect the uniqueness of S. aureus and that of the infection due to S. aureus (7,9,10,13,21).…”

mentioning

confidence: 99%

“…Among putative virulence factors of Staphylococcus aureus, staphylocoagulase (coagulase) is considered as one of the most reliable determinants for the identification and differentiation of this bacterial species from coagulase-negative staphylococci (1,12,24). It is highly reasonable to postulate that coagulase might play a role in the pathogenesis of S. aureus through its peculiar biological activity which might reflect the uniqueness of S. aureus and that of the infection due to S. aureus (7,9,10,13,21).…”

mentioning

confidence: 99%

Altered Virulence of a Pleiotropic Staphylococcus aureus Mutant with a Low Producibility of Coagulase and Other Factors in Mice

Seki

Ogasawara

Sakurada

et al. 1989

Microbiology and Immunology

View full text Add to dashboard Cite

The virulence of a pleiotropic Staphylococcus aureus mutant with an extremely low producibility of coagulase and other factors was investigated in mouse. A mutant strain, designated as CL-1, showed the same LD50 and the same intrarenal proliferation as its parental strain, when the mutant organisms were inoculated in mice in high doses. The mutant organisms, however, showed a diminished intrarenal proliferation compared with its parental organisms in low doses. This mutant strain expressed a pleiotropic phenotype such as a concomitant reduction in the producibility of coagulase, a-toxin, and Panton-Valentine leucocidin. The total effect due to the reduction in producibility of various factors on the virulence of the mutant strain was investigated with studies on the bacterial resistance to the phagocytic activity of leucocytes.A possible role of coagulase and that of some other staphylococcal exoproteins in the pathogenesis of S. aureus were discussed.

show abstract

Determination of Potential Pathogenicity of Staphylococci

Cited by 12 publications

References 0 publications

Enzymatic Detection of the Growth of Staphylococcus aureus in Foods1

Enzymatic Detection of the Growth of Staphylococcus aureus in Foods1

Comparison of Desoxyribonuclease Activity to Some Other Criteria of <i>Staphylococcus </i>Pathogenicity

Altered Virulence of a Pleiotropic Staphylococcus aureus Mutant with a Low Producibility of Coagulase and Other Factors in Mice

Contact Info

Product

Resources

About