Determination of Praziquantel in Sparus aurata L. after Administration of Medicated Animal Feed

Abstract

Praziquantel (PZQ) is an anthelmintic drug used in humans and animals against Platyhelminthes and in aquaculture in the Far East. Medicated feed is one of the most convenient forms of oral administration of drugs in aquaculture because it allows to treat a large population of fish in an easy way. However, this treatment may lead to residues in fish intended for human consumption. In this study, a liquid chromatography with tandem mass spectrometry (LC-MS/MS) method was developed in order to verify the presence of PZQ in samples of Sparus aurata after oral administration of feed treated with PZQ. The method was validated according to international guidelines. It showed good recoveries, selectivity and sensitivity (LOD and LOQ were 3.0 and 9.3 ng/g, respectively), with precision and matrix effect values ≤ 15%. This method could also be applied to determine PZQ residue in other fish species and thus to evaluate the appropriate withdrawal time in treated fish intended for human consumption.

In-feed Praziquantel treatment of grass carp (Ctenopharyngodon idella) infected with eye flukes

“…Each dose of PZQ for each aquarium was calculated from the mean weight of the fish. The PZQ concentrations were confirmed by liquid chromatography-mass spectrometry (LC-MS/MS) using the method of Baralla et al. (2020) .…”

A comparison of the detection and quantification of praziquantel via electrochemical and gas chromatography methods in freshwater and saltwater samples

“…Previous review articles can be attributed to the different approaches of detection of API. [4][5][6][7][8][9][10][11] Various methods have been developed to determine the PZQ content in different matrices, including high performance liquid chromatography (HPLC), 12,13 liquid chromatography with tandem mass spectrometry (LC-MS/MS), 14,15 near infrared spectroscopy (NIS), 16 and capillary electrophoresis (CE). 17 Electrochemical methods are also attractive since they have simpler less expensive instrumentation that can be portable.…”

Optimization and validation of high‐performance liquid chromatography using modified QuEChERS to determine anthelmintic drugs in mutton

“…Liquid chromatography (LC) combined with other discovery systems, such as UV or diode array, fluorescence or mass spectrometry (MS), is considered the best technique to quantify multiresidues of veterinary drugs (Chicoine et al, 2020). However, many methods used for the quantification of anthelmintic drug residues in animal‐derived foods, such as high‐performance liquid chromatography with ultraviolet (HPLC‐UV) spectroscopy (Cirkovic et al, 2015; Gili et al, 2014), ultra‐high‐pressure liquid chromatography (UHPLC) with different types of detector depending on the nature of the analytes (da Silva et al, 2017; Del Bianchi A. Cruz et al, 2018), micellar LC (Pawar et al, 2021), capillary zone electrophoresis tandem mass spectrometry (MS/MS) (Tejada‐Casado et al, 2018), and recently LC–MS/MS, have been considered as advanced systematic techniques to quantify anthelmintic drugs in various animal‐based foodstuffs (Baralla et al, 2020; Casey et al, 2021; Mooney et al, 2019; Yoo et al, 2021).…”