Determination of prevalence, serological diversity, and virulence of Dichelobacter nodosus in ovine footrot with identification of its predominant serotype as a potential vaccine candidate in J&amp;K, India

Wani, S.A.; Farooq, Shaheen; Kashoo, Zahid Amin; Hussain, I.; Bhat, M. Iqbal Jeelani; Rather, Mudasir Ali; Aalamgeer, S.

doi:10.1007/s11250-018-01788-9

Cited by 10 publications

(16 citation statements)

References 20 publications

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“…Serogroups H and B have been reported as the two most prevalent serogroups in the UK previously as far back as 30 years 11,14 , so the prevalence of each serogroup appears reasonably stable nationally over time. The high prevalence of serogroup B is also consistent with other countries where it is often the most prevalent serogroup [33][34][35][36][37][38] , however, serogroup H is not reported to be the most common serogroup in any other country. Despite the high prevalence of serogroups H and B a bivalent vaccine would protect only 27% of flocks in England and we conclude that one bivalent vaccine would not be effective in protecting the national flock.…”

Section: Discussionsupporting

confidence: 85%

Serogroups of Dichelobacter nodosus, the cause of footrot in sheep, are randomly distributed across England

Prosser

Monaghan

Green

et al. 2020

Sci Rep

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We present the largest and most representative study of the serological diversity of Dichelobacter nodosus in England. D. nodosus causes footrot and is one of the top five globally important diseases of sheep. The commercial vaccine, containing nine serogroups, has low efficacy compared with bivalent vaccines. Our aim was to investigate the prevalence and distribution of serogroups of D. nodosus in England to elucidate whether a bivalent vaccine could protect the national flock. Farmers from 164 flocks submitted eight interdigital swabs from eight, preferably diseased, sheep. All serogroups, A–I, were detected by PCR in 687/1150 D. nodosus positive swabs, with a prevalence of 2.6–69.3% of positive swabs per serogroup. There was a median of two serogroups per flock (range 0–6). Serogroups were randomly distributed between, but clustered within, flocks, with 50 combinations of serogroups across flocks. H and B were the most prevalent serogroups, present in > 60% of flocks separately but in only 27% flocks together. Consequently, a bivalent vaccine targeting these two serogroups would protect 27% of flocks fully (if only H and B present) and partially, if more serogroups were present in the flock. We conclude that one bivalent vaccine would not protect the national flock against footrot and, with 50 combinations of serogroups in flocks, flock-specific vaccines are necessary.

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Section: Discussionsupporting

confidence: 85%

Serogroups of Dichelobacter nodosus, the cause of footrot in sheep, are randomly distributed across England

Prosser

Monaghan

Green

et al. 2020

Sci Rep

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“…The serogroup of 34 D. nodosus positive biopsy samples were undetermined using the multiplex PCR, possibly because they belong to the serogroup M or to another yet unknown serogroup not targeted in this PCR. Serogroup B was also the most frequently found in New Zealand, United Kingdom and India (Caetano et al, 2018;Wani et al, 2019). However, in nearby Spain serogroups A and C were the most commonly detected by slide microagglutination technique (Hurtado et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

Ovine footrot in Southern Portugal: Detection of Dichelobacter nodosus and Fusobacterium necrophorum in sheep with different lesion scores

Albuquerque

Cavaco

Caetano

et al. 2022

Veterinary Microbiology

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“…Footrot has been notified as the main cause of lameness in ruminants. The prevalence of footrot lesions has been reported in many countries, such as United Kingdom (8-10%) [13], Bhutan (3.1%) [14], India (12-15%) [15][16][17], Sweden (5.8% in slaughter lambs with score ≥2) [18], Germany (42.93%) [19], Bangladesh (4.4%) [20], The prevalence of lameness in general in dairy cows has been investigated clinically in Algeria (13%) [21].…”

Section: Introductionmentioning

confidence: 99%

The prevalence, virulence, and serogroups of Dichelobacter nodosus and prevalence of Fusobacterium necrophorum in footrot lesions of sheep and cattle in Morocco

Bamouh¹,

Elkarhat

Zouagui

et al. 2023

Vet World

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Background and Aim: Footrot is a contagious disease of ruminants leading to severe economic losses. This study aimed to estimate the prevalence, virulence, and serogroups of Dichelobacter nodosus and the prevalence of Fusobacterium necrophorum in footrot lesions of sheep and cattle. Materials and Methods: A total of 106 pathogenic lesion samples were taken from 74 sheep and 32 cattle exhibiting typical footrot lesions and were analyzed for the presence of D. nodosus and F. necrophorum by real-time polymerase chain reaction (PCR). Both virulence and serogroup were estimated for D. nodosus positive samples. Results: Among the 106 samples, 89 were positive by PCR for F. necrophorum, D. nodosus, or both. Dichelobacter nodosus was detected at a rate of 78.3% versus 28.3% for F. necrophorum. Virulent D. nodosus strains were detected in 67.5% of positive samples, with a higher rate in sheep (73.4%) than in cattle (47.4%). Benign D. nodosus strains were detected in 57.8% of samples, with a lower prevalence rate in sheep (50%) than in cattle (84.2%). The positive samples of D. nodosus revealed the presence of three dominant serogroups (D, H, I) and three minor serogroups (G, C, A) by serogroup-specific multiplex PCR. Conclusion: The findings provided information on the prevalence of D. nodosus and F. necrophorum strains in footrot lesions of sheep and cattle in some regions of Morocco, which will be useful for developing an effective autovaccine for the prevention of this disease in cattle and sheep in these regions. Keywords: cattle, Dichelobacter nodosus, footrot, Fusobacterium necrophorum, polymerase chain reaction, sheep.

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Determination of prevalence, serological diversity, and virulence of Dichelobacter nodosus in ovine footrot with identification of its predominant serotype as a potential vaccine candidate in J&K, India

Cited by 10 publications

References 20 publications

Serogroups of Dichelobacter nodosus, the cause of footrot in sheep, are randomly distributed across England

Serogroups of Dichelobacter nodosus, the cause of footrot in sheep, are randomly distributed across England

Ovine footrot in Southern Portugal: Detection of Dichelobacter nodosus and Fusobacterium necrophorum in sheep with different lesion scores

The prevalence, virulence, and serogroups of Dichelobacter nodosus and prevalence of Fusobacterium necrophorum in footrot lesions of sheep and cattle in Morocco

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