Determination of procaine and tetracaine in plasma samples by micellar liquid chromatography and direct injection of sample

Escuder-Gilabert, Laura; Sagrado, S.; Medina-Hernández, M.J.; Villanueva-Camañas, R.M.

doi:10.1007/bf02490420

Cited by 9 publications

(3 citation statements)

References 22 publications

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“…In most of the works, the sample was directly introduced in LC column after the precipitation with perchloric acid 2,3,15 . The blocking of pseudocholinesterase activity was achieved with neostigmine 6 or echothiophate 15,16 , while in some works this activity was not blocked [9][10][11][12][13] . In this work articaine is analyzed by GC-FID and LC-PDA and LLE from whole blood.…”

Section: Articainementioning

confidence: 99%

Determination of Articaine in Human Blood by Gas and Liquid Chromatography and its Application in a Preliminary Pharmacokinetic Study

Manda

Popescu

Baniceru

2015

IJDDT

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Two simple, sensitive and specific gas and liquid chromatographic methods are developed and validated for quantification of articaine in human whole blood. Liquid-liquid extraction with n-hexane-isoamyl alcohol 90:10 (v/v) is used for sample preparation in both methods. GC-FID (gas chromatographic-flame ionization detection) analysis is performed with a gas chromatograph equipped with a split-splitless injector, flame-ionization detector and HP INNOwax capillary column. Reverse phase LC-PDA (liquid chromatographic-photo diode array detection) analysis is carried out using a C18 Hypersil GOLD column with two mobile phases: acetonitrile-water 10 mM ammonium acetate 50:50 (v/v), pH 7, and acetonitrile-buffer solution sodium acetate 10 mM and acetic acid 10 mM 50:50 (v/v), pH 4.7, respectively. A comparison between GC-FID and LC-PDA is performed, as well as between internal and external standard as quantification methods in LC. The best results in terms of accuracy (as recovery) and precision (as relative standard deviation) are obtained in GC (95-98% and 5.5-8.2%, respectively) with lidocaine internal standard and in LC (96-102% and 4.2-6.1%, respectively) with external standard and mobile phase pH 4.7. This method is applied in a preliminary pharmacokinetic study to three healthy volunteers to whom anesthesia with articaine is performed.

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Section: Articainementioning

confidence: 99%

Determination of Articaine in Human Blood by Gas and Liquid Chromatography and its Application in a Preliminary Pharmacokinetic Study

Manda

Popescu

Baniceru

2015

IJDDT

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“…In Chinese pharmacopoeia, procaine is titrated with sodium hydroxide and PABA in procaine hydrochloride is determined by thin layer chromatography [2]. Additionally, procaine or PABA has also been analyzed using other methods, including colorimetric [5,6], spectrophotometry [7][8][9][10], gas chromatography [11][12][13], high-performance liquid chromatography (HPLC) [14][15][16][17][18], fluorimetric detection [19][20][21], infrared spectroscopy [22], Raman spectroscopy [23], chemiluminescence [24,25], and flow-injection analysis [26]. However, only a few methods were developed for the simultaneous determination of both compounds, mainly based on HPLC [27][28][29][30][31], LC-MS/MS [32], and multiway partial least-squares coupled to residual trilinearization (N-PLS/RTL) [33].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous and sensitive determination of procaine and its metabolite for pharmaceutical quality control and pharmacokinetic research by using a graphite paste electrode

Liu

Wang

Chen

et al. 2011

J Solid State Electrochem

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A simple, rapid, sensitive, and accurate method for simultaneous electrochemical determination of procaine and its metabolite (p-aminobenzoic acid, PABA) for pharmaceutical quality control and pharmacokinetic research was developed using a graphite paste electrode. The differential pulse voltammetric results revealed that procaine and p-aminobenzoic acid, respectively, showed well-defined anodic oxidation peaks on a carbon paste electrode with a current peak separation of 155 mV at a scan rate of 100 mV s −1 . This well separation of the current peaks for these two compounds in voltammetry enables us to simultaneously determine them. Good linearity (r> 0.998) between oxidation peak current and concentration was obtained in the range of 5.0×10 −7 -5.0×10 −5 M for procaine and 5.0×10 −7 -2.0×10 −5 M for PABA in pH 4.50 acetate buffer solution. The detection limit for both analytes is 5×10 −8 M (S/N=3:1). The present voltammetric method has been successfully used to determine trace p-aminobenzoic acid in procaine hydrochloride injection and procaine in plasma with a linear relationship of current to its concentration ranging from 1.0×10 −6 to 5.0×10 −5 M (correlation coefficient of 0.9981) with a low detection limit of 5.0× 10 −7 M (S/N=3:1). This validated method is promising to the study of pharmacokinetics in Sprague-Dawley rat and rabbit plasma after an intravenous administration of procaine hydrochloride injection.

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“…It is necessary to determine local anesthetics sensitively for biochemical and clinical analysis. To date, several analytical assays have been proposed such as gas chromatography (GC) , liquid chromatography (LC) and capillary electrophoresis (CE) .…”

Section: Introductionmentioning

confidence: 99%

Separation and determination of anesthetics by capillary electrophoresis with mixed micelles of sodium dodecyl sulfate and Tween 20 using electrochemiluminescence detection

Liu

Yang

et al. 2012

Luminescence

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A simple and new method for the simultaneous determination of procaine (Pro), lidocaine (Lid), ropivacaine (Rop) and bupivacaine (Bup) was developed using capillary electrophoresis separation with mixed micelles and electrochemiluminescence detection. The use of mixed micelles of 2.0 × 10(-3) mol/L sodium dodecyl sulfate (SDS) and 8.0 × 10(-3) mol/L Tween 20 greatly improved separation selectivity. The detection sensitivities of four drugs with a Pt working electrode were increased by modification of the Pt electrode with europium(III)-doped Prussian Blue analog (Eu-PB). Under optimal conditions, the four local anesthetics were well separated and detected. The limits of detection (LOD, S/N = 3) of Pro, Lid, Rop and Bup in standard solution are 2.5 × 10(-8) , 1.3 × 10(-8) , 3.0 × 10(-8) and 4.1 × 10(-8) mol/L, respectively. The limits of quantitation (LOQ, S/N = 10) of Pro, Lid, Rop and Bup are 2.3 × 10(-7) , 1.2 × 10(-7) , 3.7 × 10(-7) and 5.6 × 10(-7) mol/L in a human urine sample, and 8.5 × 10(-7) , 6.9 × 10(-7) , 2.8 × 10(-6) and 1.1 × 10(-6) mol/L in a human serum sample, respectively. The recoveries of four drugs at different spiked concentrations in human urine and serum samples were between 86.5 and 107.6%. The proposed method has been successfully applied to determine local anesthetics in biofluids.

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Determination of procaine and tetracaine in plasma samples by micellar liquid chromatography and direct injection of sample

Cited by 9 publications

References 22 publications

Determination of Articaine in Human Blood by Gas and Liquid Chromatography and its Application in a Preliminary Pharmacokinetic Study

Determination of Articaine in Human Blood by Gas and Liquid Chromatography and its Application in a Preliminary Pharmacokinetic Study

Simultaneous and sensitive determination of procaine and its metabolite for pharmaceutical quality control and pharmacokinetic research by using a graphite paste electrode

Separation and determination of anesthetics by capillary electrophoresis with mixed micelles of sodium dodecyl sulfate and Tween 20 using electrochemiluminescence detection

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