Determination of proguanil and metabolites in small sample volumes of whole blood stored on filter paper by high-performance liquid chromatography

Kolawole, Jacob A.; Taylor, R.B.; Moody, R.R.

doi:10.1016/0378-4347(95)00293-3

Cited by 15 publications

(7 citation statements)

References 10 publications

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“…In these reports, dried blood spots are used for the therapeutic drug monitoring (TDM) of e.g. benzodiazepines [1], anti-malarial agents [2][3][4][5][6][7][8][9], anti-epileptics [10,11], immunosuppressants [12][13][14][15][16], antibiotics [17][18][19], bacteriostatics [20], analgesics [21][22][23][24], bronchodilators [25] and antiretroviral drugs [26,27]. Dried blood spot samples are obtained from a finger prick with an automatic lancet.…”

Section: Introductionmentioning

confidence: 99%

Application of dried blood spots combined with high-performance liquid chromatography coupled with electrospray ionisation tandem mass spectrometry for simultaneous quantification of vincristine and actinomycin-D

et al. 2009

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A sensitive, specific and efficient high-performance liquid chromatography-tandem mass spectrometry assay for the simultaneous determination of vincristine and actinomycin-D in human dried blood spots is presented. Dried blood spots were punched out of a collection paper with a 0.25-in.-diameter punch. The analytes were extracted from the punched-out disc using sonication during 15 min in a mixture of acetonitrile-methanol-water (1:1:1, v/v/v) containing the internal standard vinorelbine. Twenty-microlitre volumes were injected onto the HPLC system. Separation was achieved on a 50×2.1 mm ID Xbridge C 18 column using elution with 1 mM ammonium acetate-acetonitrile (70:30, v/v) adjusted to pH10.5 with ammonia and run in a gradient with methanol at a flow rate of 0.4 mL/min. HPLC run time was 6 min. The assay quantifies vincristine from 1 to 100 ng/mL and actinomycine-D from 2 to 250 ng/mL using a blood sample obtained by a simple finger prick. Validation results demonstrate that vincristine and actinomycin-D can be accurately and precisely quantified in human dried blood spots with the presented method. The assay can now be used to support clinical pharmacologic studies with vincristine and actinomycin-D.

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Section: Introductionmentioning

confidence: 99%

Application of dried blood spots combined with high-performance liquid chromatography coupled with electrospray ionisation tandem mass spectrometry for simultaneous quantification of vincristine and actinomycin-D

et al. 2009

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“…In some investigations of drug action it may be that the site of biological sample collection is geographically remote from the laboratory of analysis, where storage and transportation becomes a problem. Recently, reports have appeared in the literature dealing with the transport of quinine [1], proguanil [2] and melfloquine [3,4] by applying capillary blood samples to filter and drying. Good correlation in recovery of analyte, between samples stored in this way and those stored in the conventional frozen form has been reported [1,5].…”

Section: Introductionmentioning

confidence: 99%

Improved RP‐HPLC determination of quinine in plasma and whole blood stored on filter paper

Kolawole

Mustapha

2000

Biopharm & Drug Disp

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Analysis of quinine in plasma and whole blood samples dried on filter paper is described. Sample preparation involves liquid extraction of plasma and whole blood from the filter paper and subsequent solid-phase extraction using C8 Bond Elut cartridges. A reverse-phase liquid chromatography system with UV detection and fluorescence detection was used. The analytical characteristics of the method are reported, with a quantification limit of 0.1 microg mL(-1) and within an assay coefficient of variation of 5.6-8.4% in plasma and 6.5-12% in whole blood. Representative chromatograms are shown as a function of time for samples from human subjects after ingestion of a single 400-mg dose of quinine sulphate. Quinidine, dihydroquinine and metabolites are well separated from quinine with a resolution of above 1 (Rs>1).

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“…Taylor et al (1987) however utilized solid phase extraction procedure in the pretreatment of samples but retained the sodium lauryl sulphate ion pairing agent. Other works by Taylor et al (1990), Bergqvist and Hosptadius (2000), Kolawole et al (1995), Bergqvist et al (1998), either aimed at the determination of proguanil when used in combination with other antimalarial drugs or from very small sample volumes with little or no modifications above described methods.…”

Section: Introductionmentioning

confidence: 99%

High performance liquid chromatographic determination of proguanil after derivatisation with sodium benzoxazole-2-sulphonate

Adewuyi¹,

Olubomehin²,

Ayanniyi³

2010

Afr. J. Biotechnol.

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A simple, fast and reproducible method for the determination of proguanil using high performance liquid chromatographic with UV/Fluorescence detection is described. Proguanil was derivatised to its corresponding derivative [(N 1 -(4-chlorophenyl)-N 5 -(1 -methyl ethyl) imidocarbonimideamide-Nbenzoxazole]. The derivatisation reaction was conducted in methanol at 60°C using sodium benzoxazole-2-sulphonate under alkaline conditions. The resulting derivative was extracted with chloroform after which the extract was observed under UV lamp at 254 nm before TLC and HPLC analysis. Similarly, the derivatisation process was adapted for derivatisation of proguanil in urine sample. The reaction proceeded smoothly and rapidly. The extraction process was not cumbersome and eliminated the need for costly extraction and evaporation equipments. The resulting derivative fluorensced intensely under UV lamp. Direct HPLC analysis of the reaction mixture was found possible without interferences from excess reagent and endogeneous compounds like ammonium salts. The derivative eluted in less than seven minutes thus making the method suitable for routine use. The calibration plot was linear over the concentration range. A correlation regression of the order of 0.94 was obtained from the calibration curve which indicated a strong relationship between the instrument response and the concentration of proguanil. The discussion also summarizes the derivatisation chemistry that have not being fully explored to date but may find utility in future development of highly sensitive analytical methods for biquanide drugs.

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Determination of proguanil and metabolites in small sample volumes of whole blood stored on filter paper by high-performance liquid chromatography

Cited by 15 publications

References 10 publications

Application of dried blood spots combined with high-performance liquid chromatography coupled with electrospray ionisation tandem mass spectrometry for simultaneous quantification of vincristine and actinomycin-D

Application of dried blood spots combined with high-performance liquid chromatography coupled with electrospray ionisation tandem mass spectrometry for simultaneous quantification of vincristine and actinomycin-D

Improved RP‐HPLC determination of quinine in plasma and whole blood stored on filter paper

High performance liquid chromatographic determination of proguanil after derivatisation with sodium benzoxazole-2-sulphonate

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