Determination of protein: A modification of the lowry method that gives a linear photometric response

Hartree, E. F.

doi:10.1016/0003-2697(72)90094-2

Cited by 5,222 publications

(1,618 citation statements)

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“…The chlorophyll-a and phaeopigment contents were determined following Lorentzen (1967). The protein and carbohydrate contents were determined following Hartree (1972) and Dubois et al (1956) respectively. The pigment and biochemical composition analyses were carried out using a Varian DSM90 spectrophotometer.…”

Section: Laboratory Proceduresmentioning

confidence: 99%

Stressors affecting the macrobenthic community in Rapallo Harbour (Ligurian Sea, Italy)

Harriague¹,

Misic²,

Petrillo³

et al. 2007

Sci. Mar.

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SUMMARY: An annual survey was carried out in Rapallo Harbour (Ligurian Sea, NW Mediterranean) to study the main stressors affecting the macrobenthic communities. The harbour was characterised by a relevant organic enrichment (between 51.9 mg g -1 and 109.6 mg g -1 ), which did not affect the water column oxygenation, and low but detectable heavy-metal contamination (on average Cu=135.0 μg g -1 and V=23.8 μg g -1 ). The seasonal changes in the heavy metal concentrations suggested a significant increase in tourist activities (i.e. boat movements) during summer and autumn. In the inner, more protected sampling site, the harbour structures and the vessel movements increased both organic enrichment and sediment instability, resulting in macrobenthic communities dominated by indicator species associated with these stressors. In addition, during summer and autumn, despite a relevant chlorophyll-a flux to the sediment, the settling material was dominated by phaeopigments and refractory proteins, which reduced its trophic value for benthic consumers. In the outer part of the harbour the main stressor was a polluted freshwater inflow, as indicated by the dominance of indicator species such as Capitella spp. (on average 79.5%), Neanthes caudata, Malacoceros girardi and Diopatra neapolitana.

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Section: Laboratory Proceduresmentioning

confidence: 99%

Stressors affecting the macrobenthic community in Rapallo Harbour (Ligurian Sea, Italy)

Harriague¹,

Misic²,

Petrillo³

et al. 2007

Sci. Mar.

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“…EROD activity was measured at 20°C as previously described (Monod et al, 1987) using a 7-ethoxyresorufin final concentration of 5/tM in a 50 raM-phosphate buffer, pH 8.0. Protein concentration was determined according to Hartree (1972) with bovine serum albumin as a standard.…”

Section: Animals Rainbow Trout (Oncorhynchus Mykiss)mentioning

confidence: 99%

DNA adduct formation and 7-ethoxyresorufin O-deethylase induction in primary culture of rainbow trout hepatocytes exposed to benzo[a]pyrene

Masfaraud¹,

Devaux²,

Pfohl‐Leszkowicz³

et al. 1992

Toxicology in Vitro

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Abstract--The formation of DNA adducts, using the 32p-postlabeiling assay, and induction of 7-ethoxyresorufin O-deethylase (EROD) were investigated in a primary culture of rainbow trout hepatocytes exposed to benzo[a]pyrene (B[a]P). Concentrations of 0.1 and 1/zM-B[a]P were shown to induce EROD whereas 10 #M was an inhibitory concentration. DNA adducts were detected for 12 hr to 72 hr after exposure to 1 #M-B[a]P whereas EROD activity was increased 36 hr after treatment. The pattern of adducts was shown to be identical to that obtained after B[a]P treatment of rainbow trout in vivo, as demonstrated by co-chromatography of the adducts. Pre-exposure of hepatocytes for 48 hr to fl-naphthoflavone (~NF) and subsequent 24-hr exposure to 1 ~uM-B[a]P did not lead to increased DNA adduct formation although flNF treatment led to a 3.4-fold induction of EROD activity at the time of B[a]P addition. This study suggests that primary culture of rainbow trout hepatocytes provides a suitable method for studying DNA adduct formation and its modulating factors/n vitro.

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“…Protein estimation was carried out according to Lowry's method [12,13] using Bovine serum Albumin solution (BSA) as a standard.…”

Section: Protein Estimationmentioning

confidence: 99%

Activity of Hydrolytic Enzymes in Various Regions of Normal Human Brain Tissue

2012

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The activity of six hydrolytic enzymescarboxyl esterase, acid phosphatase, alkaline phosphatase, b-galactosidase, b-glucosidase and b-hexosaminidase, were studied in different regions of the normal human brain tissue obtained at autopsy. Protein estimation and activities of the hydrolytic enzymes with respective substrates were assayed by spectrophotometric and spectroflourometric methods. Amongst the eight regions of the brain-frontal, parietal, occipital, temporal, thalamus, cerebellum and hippocampus, the pineal gland showed highest activity for all hydrolytic enzymes studied except for carboxyl esterase. Among six hydrolases studied, hexosaminidase exhibited highest activity in all regions of the human brain while alkaline phosphatase activity was the least amongst all regions studied. A majority of the enzymes studied showed higher activity in gray matter as compared to the white matter except acid phosphatase and b-glucosidase which exhibited higher activity in the white matter. The most significant finding in the present study was the high activity of all hydrolytic enzymes noted in the pineal gland as compared to all other regions of the human brain. Such a finding has not been hitherto reported earlier in human brain tissue samples. If the specific activities of these enzymes are to be considered as any functional index, then pineal gland may be more metabolically active tissue with respect to the hydrolytic function as compared to the other regions of the brain.

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Determination of protein: A modification of the lowry method that gives a linear photometric response

Cited by 5,222 publications

References 5 publications

Stressors affecting the macrobenthic community in Rapallo Harbour (Ligurian Sea, Italy)

Stressors affecting the macrobenthic community in Rapallo Harbour (Ligurian Sea, Italy)

DNA adduct formation and 7-ethoxyresorufin O-deethylase induction in primary culture of rainbow trout hepatocytes exposed to benzo[a]pyrene

Activity of Hydrolytic Enzymes in Various Regions of Normal Human Brain Tissue

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