Determination of proteins at nanogram levels by their quenching effect on the chemiluminscence reaction between luminol and hydrogen peroxide with manganese-tetrasulfonatophthalocyanine as a new catalyst

Abstract

The manganese-tetrasulfonatophthalocyanine (MnTSPc) catalyzed luminol-hydrogen peroxide chemiluminescence (CL) systems can be quenched in the presence of proteins. A highly sensitive CL quenching method has been developed for the determination of proteins. Under optimum conditions, the linear ranges of the calibration curves were 0.1-20 microg/mL for human serum albumin (HSA), 0.2-20 microg/mL for human gamma-IgG, and 0.5-50 microg/mL for the bovine serum albumin (BSA) with the corresponding detection limits were 1.9 ng/mL, 2.7 ng/mL, and 3.4 ng/mL. The method has been applied to the analysis of total proteins in human serum samples and the results were in good agreement with clinical data provided.

Quantum Dot–Polymer Bead Composites for Biological Sensing Applications

“…Finally, to benchmark the method, three individual human serum samples were assessed for total protein content using this RLS method. The values obtained had excellent agreement with values arrived at using the more conventional Bradford assay [58].…”

Singlet Oxygen Generation in Ferriporphyrin-Polymer Dots Catalyzed Chemiluminescence System for Cancer Therapy

“…It was reported that some metalloporphyrins such as hemin have strong catalytic activity on luminol-H 2 O 2 CL reaction. 44 The molecular structures of the metalloporphyrins affect their catalytic activity, including their coordination center metal ions and the exterior porphyrin structure such as the carboxyl group, electronegativity, conjugated system, and their steric hindrance. Metalloporphyrin-polymer dots were prepared by using nanoprecipitation, and their catalytic CL activities to the L012-H 2 O 2 reaction were systematically investigated.…”

A novel chemiluminescent flow injection analysis of trace amounts of rutin by its inhibition of the luminol–hydrogen peroxide reaction catalyzed by tetrasulfonated colbalt phthalocyanine

“…2, curve b), which means that the process of hydrogen peroxide transferring into intermediate species can be accelerated in the presence of CoTSPc (23,24). On the other hand, when rutin was added into this luminol CL system (Fig.…”

“…In 1990, Hara et al first developed the application of metallophthalocyanine compounds as catalysts in the CL reaction between luminol and hydrogen peroxide, and furthermore as labeling reagents in CL immunoassay (22). Metallophthalocyanine has also been used for a wide range of applications in CL analysis (23)(24)(25).…”