Determination of psilocybin in Psilocybe semilanceata by capillary zone electrophoresis

Taxonomic Identification of Hallucinogenic Mushrooms Seized on the Illegal Market Using a DNA-Based Approach and LC/MS-MS Determination of Psilocybin and Psilocin

“…LC/MS-MS analysis of the three extracts corresponding to the three different parts of the mushrooms gave the results reported in Table 3. The concentration of psilocybin ranged from 0.5 to 1.4% while that of psilocin from 1.3 to 2.5%, confirming literature data [13]. It is possible to note that the concentration of psilocin is higher in the cap and in the distal part of the stem (near to the soil) than in the part of the stem proximal to the cap.…”

“…The psychoactive principles of Psilocybe mushrooms, namely psilocybin and psilocin, were isolated and identified by Hofmann in 1958 [10]. Psilocin and psilocybin are naturally occurring indoles ( Figure 1) found in several species of mushrooms [11,12] at concentrations of up to 0.5% m/m and 2% m/m, respectively [13]. Psilocybin is rapidly dephosphorylated to psilocin in vivo [14], with the latter compound being structurally related to the neurotransmitter serotonin ( Figure 2) which gives rise to its comparable human metabolism [15].…”

Occurrence and use of hallucinogenic mushrooms containing psilocybin alkaloids

“…CZE, 57 cm×50 µm fused silica capillary Pedersen-Bjergaard et al, 1997 Methanol, grinding and storage over night HPLC, C12 col.…”

“…Recently Pedersen-Bjergaard et al (1997) developed a capillary zone electrophoretic method to determine psilocybin and other indole alkaloids in Psilocybe semilanceata. Although this method seems to be a promising alternative to high-performance liquid chromatography it did not allow a simultaneous determination of psilocybin and psilocin.…”

Discovery of a new caerulescent Psilocybe mushroom in Germany: Psilocybe germanica sp.nov.

“…As far as it is currently known, this trimethylammonium analog of psilocybin has only been reported to occur in the hallucinogenic species Inocybe aeruginascens Babos. [13,21] Psilocybe semilanceata is the best studied psychoactive mushroom species in the world [1,14,18,19,17,22,23] and is known to contain psilocybin and baeocystin (Table 1). Previous investigations in 1994 have shown that the use pure methanol yielded the authentic indole derivatives from dried mushrooms and the use of aqueous solvents resulted in hydrolysis of psilocybin and baeocystin to give psilocin.…”