Determination of puerarin in biological samples and its application to a pharmacokinetic study by flow‐injection chemiluminescence

Abstract

A simple and sensitive flow injection-chemiluminescence (FI-CL) method has been developed for the determination of puerarin, based on the fact that puerarin can greatly inhibit CL of the luminol-H₂O₂-haemoglobin system. The inhibition of CL intensity was linear to the logarithm of the concentration of puerarin in the range 0.08-10.0 μg/mL (r² = 0.9912). The limit of detection was 0.05 μg/mL (3σ) and the relative standard deviation (RSD) for 1.0 μg/mL (n = 11) of puerarin solution was 1.4%. Coupled with solid-phase extraction (SPE) as the sample pretreatment, the determination of puerarin in biological samples and a preliminary pharmocokinetic study of puerarin in rats were performed. The recoveries for plasma and urine at three different concentrations were 89.2-110.0% and 91.4-104.8%, respectively. The pharmacokinetics of puerarin in plasma of rat coincides with the two-compartment open model. The T(½α) , T(½β) , CL/F, V(Z/F), AUC(₀₋t), MRT₀₋∞, T(max) and C(max) were 0.77 ± 0.21 h, 7.55 ± 2.64 h, 2.43 ± 1.02 L/kg/h, 11.40 ± 3.45 L/kg, 56.67 ± 10.65 mg/h/L, 5.04 ± 2.78 h, 1.00 ± 0.35 h and 19.70 ± 4.67 μg/mL, respectively.

Flow injection chemiluminescence determination of 2‐methoxyestradiol based on inhibition of luminol‐potassium ferricyanide reaction

“…Results were summarized in Table 3 as well as the results obtained by using hydrophilic interaction chromatography. It was found that there was no significant difference between the results obtained by the two methods (23,24).…”

Pharmacokinetics and drug delivery systems for puerarin, a bioactive flavone from traditional Chinese medicine

“…Species specificity was found in pharmacokinetics of puerarin. The pharmacokinetics of puerarin in plasma of rats and dogs coincides with the two-compartment open model (Jin & Zhu, 1992; Yang et al., 2011; Ouyang et al., 2012), while in rabbits was best fitted to the three-compartment open model (Jin & Zhu, 1992).…”

Sensitive determination of 2‐methoxyestradiol in pharmaceutical preparations and serum samples using flow injection chemiluminescence

“…Plasma samples were obtained from the Red Cross Blood Centre in Henan Province (China). Three different known amounts of standard solution of 2ME (5.0 × 10 ‐4 mol/L) were added to 200 μL serum samples, and then treated with triple methanol and thoroughly vortex mixed for 3 min . After centrifugation at 12,000 rpm for 15 min at 4°C, the supernatants were collected and diluted with water so that the final concentration of the drug was within the linear range of determination.…”