Determination of Punicalagin Isomers in Pomegranate Husk

Abstract

Punicalagins are the main ingredients of phenolic compounds in pomegranate (Punica granatum L.) husk. A simple and accurate method for punicalagin analysis based on ethanol extraction and RP-LC using linear gradient of methanol in 0.1% TFA solution was established. The feasibility of this procedure was tested by analyzing the punicalagin level both in fresh pomegranate husk collected from different provinces in China and dried husk from a drugstore. The content of each isomer and total content of punicalagins in husk were determined. The mean value of punicalagins content in pomegranate husk was 82.4 mg g -1

Comparison of Fresh and Commercial Pomegranate Juices from Mollar de Elche Cultivar Grown under Conventional or Organic Farming Practices

“…The results showed that α-punicalagin was more abundant than β-punicalagin. The most abundant compounds among the pomegranate polyphenols are punicalagin isomers [29], which have important antioxidant and atherosclerotic biological properties [30]. The results from the present study showed significant difference among fresh and commercial pomegranate juice, as well as conventional and organic agricultural practices.…”

Pressurised water extraction of polyphenols from pomegranate peels

“…Punicalagin A and punicalagin B interconvert rapidly when in solution to render approximately 30% A and 70% B (Gil, TomasBarberan, Hess-Pierce, Holcroft, & Kader, 2000). These two compounds are named also as a and b anomers of punicalagin (Lu, Ding, & Yuan, 2008;Seeram, Lee, Hardy, & Heber, 2005). In the present study, the proportion of two anomers based on the peak areas were determined approximately as 35% A and 65% B, respectively.…”

“…The accuracy of the HPLC method was tested by recovery rates and found as 90.0%, 87.3%, and 95.9% for gallic acid, punicalagin, and ellagic acid, respectively. Punicalagin content of pomegranate peels was reported as between 39.8 and 121.5 mg/g for 16 pomegranate varieties (Lu et al, 2008). Polyphenols of pomegranate peels (only punicalagin A and punicalagin B) were extracted with aqueous ethanol and determined by HPLC in aforementioned study.…”

Bioactive compound composition of pomegranate fruits removed during thinning

“…The most abundant of the polyphenolic compound in pome-419 granate is punicalagin; punicalagin together with ellagic acid are 420 potent antioxidants, anticancer and have anti-atherosclerotic 421 biological properties (Lu et al, 2008). Furthermore, ellagic acid 422 has shown to be effective as an inhibitor of lipid peroxidation 423 (Hä kkinen et al, 2000;Seeram et al, 2005).…”