Determination of quercetin in human plasma after ingestion of commercial canned green tea by semi‐micro HPLC with electrochemical detection

Jin, Dongri; Hakamata, Hideki; Takahashi, Kouji; Kotani, Akio; Kusu, Fumiyo

doi:10.1002/bmc.370

Cited by 45 publications

(24 citation statements)

References 23 publications

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“…Analytical methods have included LC/MS [4,5], LC/MS/MS [6][7][8], HPLC with UV detection [9], HPLC with fluorescent detection [10], HPLC with electrochemical detection [7,[10][11][12][13][14] and HPLC-radiocounting and tandem mass spectrometry [11]. Following onion ingestion, Witting et al [6] attempted to analyze unchanged quercetin, but were only able to detect quercetin conjugates while Mullen et al [7] reported trace levels of unchanged quercetin in one subject.…”

Section: Introductionmentioning

confidence: 97%

Liquid chromatography–tandem mass spectroscopy assay for quercetin and conjugated quercetin metabolites in human plasma and urine

Wang

Morris

2005

Journal of Chromatography B

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Section: Introductionmentioning

confidence: 97%

Liquid chromatography–tandem mass spectroscopy assay for quercetin and conjugated quercetin metabolites in human plasma and urine

Wang

Morris

2005

Journal of Chromatography B

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“…A lower detection limit of 1.5 pg (recalculated as 15 mg/mL of sample) was reached with electrochemical detection using the redox properties of the phenolic compounds [24]. Figure 2 shows a chromatogram of blood plasma from volunteers who drank green tea after enzymatic hydrolysis of glucuronides and quercetin sulfates.…”

Section: Analytical Methods For Biological Samples From Pk Studies Ofmentioning

confidence: 99%

Pharmacokinetics of Quercetin and Other Flavonols Studied by Liquid Chromatography and LC-MS (a Review)

Guglya

2014

Pharm Chem J

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Modern analytical methods used to study the pharmacokinetics of quercetin and other flavonols, i.e., biologically active compounds that exhibit various therapeutic properties, were reviewed. The preparation of biological fluids and tissues for analysis, chromatography conditions, and mass spectrometric detection for various flavonols as the aglycons and glycosides and their metabolites were discussed. Quantitative analyses of flavonol concentrations in biological samples and their mass spectrometric identification in in vitro and in vivo studies during tests with pure compounds and multi-constituent plant extracts were presented as typical examples.Keywords: quercetin, flavonols, flavonoids, flavonoid pharmacokinetics, bioanalytical methods, liquid chromatography-mass spectrometry (LC-MS/MS).Flavonols are a subclass of flavonoids, a varied and broad class of polyphenolic compounds that includes the flavonol quercetin, one of the most widely distributed and studied representatives. These compounds were well known over 100 years ago as plant pigments. However, the discovery in the 1990s of the antioxidant properties of flavonoids stimulated renewed interest in them. They are capable of neutralizing free radicals, which is responsible for their various therapeutic properties [1,2].A pharmacokinetic (PK) study in vivo in humans and experimental animals, i.e., absorption, distribution, metabolism, and elimination, is a necessary component of the evaluation of the mechanisms of possible therapeutic or toxic action of a biologically active compound. The PK parameters of drug candidates are related to the effectiveness and administration mode in order to establish the optimum concentrations.The properties of quercetin and other flavonols were briefly characterized and the development in the last decade of methods for determining the concentrations and identifying flavonols in biological media during PK studies were discussed in the review. Structure, properties and natural sources of flavonolsFlavonoids are secondary plant metabolites and share common biosynthetic pathways. They are responsible for the color of fruits and leaves. Surface tissues of plants are especially rich in them [3]. They are involved in the photosynthesis, proliferation, and death (through apoptosis) of plant cells. It is assumed that the main biological role of flavonoids is to protect plants from aggressive environmental factors (UV radiation, infectious agents).The variety of flavonoids is explained by the fact that most of them occur in plants as O-or C-glycosides. The aglycon is a 15-carbon skeleton, the principal elements of which are two aromatic rings joined by a pyran ring. The last determines to which subclass one flavonoid or another belongs. The classification includes the subclasses flavones, flavonols, flavanones (catechins), anthocyanidins, isoflavones, and flavanonols [4].

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“…Electrochemical detection has also been applied to biological samples, as described by Jin et al The aim of the study was to validate and apply a method for the quantification of quercetin in human plasma after the ingestion of a commercial canned green tea (Jin et al, 2004). The analysis was performed by an HPLC system coupled with electrochemical detection.…”

Section: Electrochemical Detectionmentioning

confidence: 99%

Improved Characterization of Polyphenols Using Liquid Chromatography

Lamuela‐Raventós

Vallverdú‐Queralt

Jáuregui

et al. 2014

Polyphenols in Plants

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Determination of quercetin in human plasma after ingestion of commercial canned green tea by semi‐micro HPLC with electrochemical detection

Cited by 45 publications

References 23 publications

Liquid chromatography–tandem mass spectroscopy assay for quercetin and conjugated quercetin metabolites in human plasma and urine

Liquid chromatography–tandem mass spectroscopy assay for quercetin and conjugated quercetin metabolites in human plasma and urine

Pharmacokinetics of Quercetin and Other Flavonols Studied by Liquid Chromatography and LC-MS (a Review)

Improved Characterization of Polyphenols Using Liquid Chromatography

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