Determination of Quinic Acids in <i>Helichrysum arenarium</i> (L.) Moench by Ultrafiltration Affinity and Ultra-High-Performance Liquid Chromatography Coupled with Quadrupole-Time-of-Flight Mass Spectrometry (UF-UPLC-Q-TOF-MS)

Yang, Jinxiang; Qi, Yunyun; Li, Hongye; Jiang, Min; Zhu, Yong; Xue, Rui; Yu, Lili; Cui, Wen; Han, Bo

doi:10.1080/00032719.2020.1781876

Cited by 4 publications

(3 citation statements)

References 33 publications

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“…Lonicerae japonicae , buds; Achyrocline satureioides (Lam) DC., aerial parts; Anthemis tinctoria , aerial parts; kangfuxiaoyanshuan; Aster tataricus L., roots and rhizomes; Angelicae Pubescentis Radix, roots; Valeriana jatamansi Jones, roots and rhizomes; Helichrysum arenarium (L.), flowers (Ma et al., 2018; Martinez‐Busi et al., 2019; Orlando et al., 2019; Rho et al., 2020; Wang, Yao, et al., 2021; Xie et al., 2019; Yang, Hou, et al., 2020; Yang, Liu, et al., 2020; Yang et al., 2021)…”

Section: Chemistrymentioning

confidence: 99%

Caffeoylquinic acids: chemistry, biosynthesis, occurrence, analytical challenges, and bioactivity

et al. 2021

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SUMMARY Caffeoylquinic acids (CQAs) are specialized plant metabolites we encounter in our daily life. Humans consume CQAs in mg‐to‐gram quantities through dietary consumption of plant products. CQAs are considered beneficial for human health, mainly due to their anti‐inflammatory and antioxidant properties. Recently, new biosynthetic pathways via a peroxidase‐type p‐coumaric acid 3‐hydroxylase enzyme were discovered. More recently, a new GDSL lipase‐like enzyme able to transform monoCQAs into diCQA was identified in Ipomoea batatas. CQAs were recently linked to memory improvement; they seem to be strong indirect antioxidants via Nrf2 activation. However, there is a prevalent confusion in the designation and nomenclature of different CQA isomers. Such inconsistencies are critical and complicate bioactivity assessment since different isomers differ in bioactivity and potency. A detailed explanation regarding the origin of such confusion is provided, and a recommendation to unify nomenclature is suggested. Furthermore, for studies on CQA bioactivity, plant‐based laboratory animal diets contain CQAs, which makes it difficult to include proper control groups for comparison. Therefore, a synthetic diet free of CQAs is advised to avoid interferences since some CQAs may produce bioactivity even at nanomolar levels. Biotransformation of CQAs by gut microbiota, the discovery of new enzymatic biosynthetic and metabolic pathways, dietary assessment, and assessment of biological properties with potential for drug development are areas of active, ongoing research. This review is focused on the chemistry, biosynthesis, occurrence, analytical challenges, and bioactivity recently reported for mono‐, di‐, tri‐, and tetraCQAs.

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Section: Chemistrymentioning

confidence: 99%

Caffeoylquinic acids: chemistry, biosynthesis, occurrence, analytical challenges, and bioactivity

et al. 2021

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“…The anti-atherosclerotic activities of individual flavonoids derived from H. arenarium have also been described (Mao et al, 2017). Yang et al (2021) identified compounds in the H. arenarium extract that exhibited anti-obesity activity and showed that all of the substances found were derivatives of caffeic acid, with isochlorogenic acid showing the most potent activity. Helichrysum is rich in polyphenols and their infusions have a beneficial effect on patients with metabolic syndrome.…”

Section: Introductionmentioning

confidence: 99%

The effect on the organism of rats of adding Helichrysum arenarium inflorescences to a hypercaloric diet, high in sugar and fat

Lieshchova,

Brygadyrenko

2023

Biosys. divers.

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Obesity increases the risk of developing various pathological conditions such as metabolic syndrome, type 2 diabetes, non-alcoholic fatty liver disease, and cardiovascular disease. For mild correction and even treatment of obesity, drugs based on medicinal plants are increasingly being used. This work aims to determine the overall effect of Helichrysum arenarium inflorescences on physiological activity and metabolic processes in model animals fed a diet rich in fat and carbohydrates. In a 27-day experiment on 18 laboratory rats fed a diet with high-fat content and 20% fructose solution, we determined the effect of 0.4% and 4.0% dry inflorescences of H. arenarium from the feed mass on the animals' physiological activity and metabolic processes. The body weight of rats in the control group reached 121.7% of the initial body weight; when consuming 0.4% and 4.0% H. arenarium in the diet, this indicator was lower – 109.6% and 111.2%, respectively. Dry inflorescences of H. arenarium in the rats’ diet at a dose of 0.4% caused a decrease in the relative mass of the heart and thymus and an increase in the relative mass of the caecum and colon. A diet with the addition of H. arenarium caused a change in blood biochemical parameters: an increase in the urea concentration and urea nitrogen, and a decrease in the glucose concentration and protein coefficient. Helichrysum arenarium at both concentrations significantly increased the gamma-glutamyltransferase and alkaline phosphatase activity with a decrease in alpha-amylase activity in the blood. A 0.4% dose of inflorescences of the food mass caused a decrease in AST activity, and 200 g sharply increased blood ALT activity. The consumption of dried H. arenarium inflorescences decreased hematocrit, haemoglobin concentration, erythrocyte and platelet counts, and increased the percentage of eosinophils and monocytes. A decrease in physical activity and an intensification of emotional state were found in the animals after eating dry inflorescences of H. arenarium, regardless of the dose compared with the control group of animals. Significant changes in the orientation activity of the animals were not observed. The obtained results show that adding dry H. arenarium inflorescences as a food supplement to a high-calorie diet is safe, does not cause pathological changes and side effects, and significantly affects metabolic processes. This provides theoretical support for the use of dried H. arenarium inflorescences for the production of nutraceutical and pharmacological products for the correction of metabolic disorders in humans and animals. The doses and duration of their use require further research.

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“…In the ultrafiltration step, the ligand−receptor complex can be separated from the unbound small molecules, and the active small molecules in the complex can be released by adding an organic solvent. The ligands can then be identified by the LC−MS step, and the active small molecules can be initially identified [ 15 ]. Molecular docking was used to simulate the binding of the active components of the monomer to AChE.…”

Section: Introductionmentioning

confidence: 99%

Efficient Combination of Complex Chromatography, Molecular Docking and Enzyme Kinetics for Exploration of Acetylcholinesterase Inhibitors from Poria cocos

Hou

Liu

et al. 2023

Molecules

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Poria cocos (P. cocos) is a traditional Chinese medicinal product with the same origin as medicine and food. It has diuretic, anti-inflammatory and liver protection properties, and has been widely used in a Chinese medicine in the treatment of Alzheimer’s disease (AD). This study was conducted to explore the activity screening, isolation of acetylcholinesterase inhibitors (AChEIs), and in vitro inhibiting effect of P. cocos. The aim was to develop a new extraction process optimization method based on the Matlab genetic algorithm combined with a traditional orthogonal experiment. Moreover, bio−affinity ultrafiltration combined with molecular docking was used to screen and evaluate the activity of the AChEIs, which were subsequently isolated and purified using high-speed counter−current chromatography (HSCCC) and semi−preparative high-performance liquid chromatography (semi−preparative HPLC). The change in acetylcholinesterase (AChE) activity was tested using an enzymatic reaction kinetics experiment to reflect the inhibitory effect of active compounds on AChE and explore its mechanism of action. Five potential AChEIs were screened via bio−affinity ultrafiltration. Molecular docking results showed that they had good binding affinity for the active site of AChE. Meanwhile, the five active compounds had reversible inhibitory effects on AChE: Polyporenic acid C and Tumulosic acid were non-competitive inhibitors; 3−Epidehydrotumulosic acid was a mixed inhibitor; and Pachymic acid and Dehydrotrametenolic acid were competitive inhibitors. This study provided a basis for the comprehensive utilization of P. cocos and drug development for the treatment of AD.

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Determination of Quinic Acids in Helichrysum arenarium (L.) Moench by Ultrafiltration Affinity and Ultra-High-Performance Liquid Chromatography Coupled with Quadrupole-Time-of-Flight Mass Spectrometry (UF-UPLC-Q-TOF-MS)

Cited by 4 publications

References 33 publications

Caffeoylquinic acids: chemistry, biosynthesis, occurrence, analytical challenges, and bioactivity

Caffeoylquinic acids: chemistry, biosynthesis, occurrence, analytical challenges, and bioactivity

The effect on the organism of rats of adding Helichrysum arenarium inflorescences to a hypercaloric diet, high in sugar and fat

Efficient Combination of Complex Chromatography, Molecular Docking and Enzyme Kinetics for Exploration of Acetylcholinesterase Inhibitors from Poria cocos

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