“…KTI, previously dissolved in physiological saline, was emulsified (1 mg/ml, 100 µg) in FCA for the first immunization of each mouse. Booster immunizations were performed every 21 d, and the immunogen dose was adjusted to 50 µg KTI emulsified in FIA (Guan et al, 2015;Peng et al, 2015). After 7-10 d of the third immunization, sera were collected from the tail of each mouse, and the titer was detected using indirect ELISA (Kong, Song, et al 2015;Kong, Liu, Xing, & Xu, 2015).…”