Determination of rat blood S-d-lactoylglutathione by a column-switching high-performance liquid chromatography with precolumn fluorescence derivatization with 4-fluoro-7-nitro-2,1,3-benzoxadiazole

Uchino, Erika; Fukushima, Takeshi; Tsunoda, Makoto; Santa, Tomofumi; Imai, Kazuhiro

doi:10.1016/j.ab.2004.03.062

Cited by 8 publications

(7 citation statements)

References 29 publications

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“…Contrasting results have been reported on the effects of incubation with high glucose levels on glyoxalase system in different experimental models. In vivo studies in streptozotocin induced diabetic rat, an increase in GlxI and GlxII activity in red blood cells and skeletal muscle was observed while the activity decreased in the liver [ 44 , 45 ]. Other authors have demonstrated that GlxI increased in glomeruli of diabetic mice, while decreased in renal cortex [ 46 ].…”

Section: Discussionmentioning

confidence: 99%

Protection of Polyphenols against Glyco-Oxidative Stress: Involvement of Glyoxalase Pathway

et al. 2020

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Chronic high glucose (HG) exposure increases methylglyoxal (MGO)-derived advanced glycation end-products (AGEs) and is involved in the onset of pathological conditions, such as diabetes, atherosclerosis and chronic-degenerative diseases. Under physiologic conditions the harmful effects of MGO are contrasted by glyoxalase system that is implicated in the detoxification of Reactive Carbonyl Species (RCS) and maintain the homeostasis of the redox environment of the cell. Polyphenols are the most abundant antioxidants in the diet and present various health benefits. Aims of the study were to investigate the effects of HG-chronic exposure on glyco-oxidation and glyoxalase system in intestinal cells, using CaCo-2 cells. Moreover, we studied the effect of apple polyphenols on glyco-oxidative stress. Our data demonstrated that HG-treatment triggers glyco-oxidation stress with a significant increase in intracellular Reactive Oxygen Species (ROS), lipid peroxidation, AGEs, and increase of Glyoxalase I (GlxI) activity. On the contrary, Glyoxalase II (GlxII) activity was lower in HG-treated cells. We demonstrate that apple polyphenols exert a protective effect against oxidative stress and dicarbonyl stress. The increase of total antioxidant capacity and glutathione (GSH) levels in HG-treated cells in the presence of apple polyphenols was associated with a decrease of GlxI activity.

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Section: Discussionmentioning

confidence: 99%

Protection of Polyphenols against Glyco-Oxidative Stress: Involvement of Glyoxalase Pathway

et al. 2020

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“…Ellman's reagent is widely used for the analysis of thiols in biological samples via the determination of the liberated thionitrobenzoate anion [126]. HPLC with pre-column derivatization with fluorogenic reagents, such as ammonium 7-fluoro-2,1,3-benzoxadiazole-4-sulfonate (SBD-F) has become the most popular method for thiol analysis [127][128][129][130].…”

Section: Derivatizationmentioning

confidence: 99%

Chromatographic and mass spectrometric analysis of glutathione in biological samples

Iwasaki

Saito

Nakano

et al. 2009

Journal of Chromatography B

101

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“…After the deproteinization of samples, either an anion-exchange solid-phase extraction was used prior to reverse-phase HPLC [105] or SDL was simply separated and quantified using an isocratic HPLC procedure [106]. Recently, a column-switching HPLC method with a precolumn fluorescence derivatization with 4-fluoro-7-nitro-2,1,3-benzoxadiazole, a fluorogenic reagent, has been worked out in which the deproteinization of the samples is also essentially involved [107]. In healthy humans, the SDL concentration of blood measured by HPLC has been found to be as high as 16.5 ± 4.4 µM [101].…”

Section: The Measurement Of S-d-lactoylglutathione In Biological Samplesmentioning

confidence: 99%

“…In healthy humans, the SDL concentration of blood measured by HPLC has been found to be as high as 16.5 ± 4.4 µM [101]. HPLC-based methods have been used to detect SDL concentrations in normal human as well as rat blood and in promyelocytic leukemia cells [106][107][108][109][110][111].…”

Section: The Measurement Of S-d-lactoylglutathione In Biological Samplesmentioning

confidence: 99%

Metabolic Shades of S-D-Lactoylglutathione

Kalapos¹,

Antognelli

Bari

2022

Antioxidants

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S-D-lactoylglutathione (SDL) is an intermediate of the glutathione-dependent metabolism of methylglyoxal (MGO) by glyoxalases. MGO is an electrophilic compound that is inevitably produced in conjunction with glucose breakdown and is essentially metabolized via the glyoxalase route. In the last decades, MGO metabolism and its cytotoxic effects have been under active investigation, while almost nothing is known about SDL. This article seeks to fill the gap by presenting an overview of the chemistry, biochemistry, physiological role and clinical importance of SDL. The effects of intracellular SDL are investigated in three main directions: as a substrate for post-translational protein modifications, as a reservoir for mitochondrial reduced glutathione and as an energy currency. In essence, all three approaches point to one direction, namely, a metabolism-related regulatory role, enhancing the cellular defense against insults. It is also suggested that an increased plasma concentration of SDL or its metabolites may possibly serve as marker molecules in hemolytic states, particularly when the cause of hemolysis is a disturbance of the pay-off phase of the glycolytic chain. Finally, SDL could also represent a useful marker in such metabolic disorders as diabetes mellitus or ketotic states, in which its formation is expected to be enhanced. Despite the lack of clear-cut evidence underlying the clinical and experimental findings, the investigation of SDL metabolism is a promising field of research.

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Determination of rat blood S-d-lactoylglutathione by a column-switching high-performance liquid chromatography with precolumn fluorescence derivatization with 4-fluoro-7-nitro-2,1,3-benzoxadiazole

Cited by 8 publications

References 29 publications

Protection of Polyphenols against Glyco-Oxidative Stress: Involvement of Glyoxalase Pathway

Protection of Polyphenols against Glyco-Oxidative Stress: Involvement of Glyoxalase Pathway

Chromatographic and mass spectrometric analysis of glutathione in biological samples

Metabolic Shades of S-D-Lactoylglutathione

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