Determination of Retinal and Vitreous Temperature in Vitrectomy

Landers, Maurice B.; Watson, Jessica S.; Ulrich, J. Niklas; Quiroz‐Mercado, Hugo

doi:10.1097/iae.0b013e31821c3ee0

Cited by 32 publications

(22 citation statements)

References 6 publications

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“…5 . The strict measurements taken of retinal temperature immediately adjacent to the fovea by Landers et al during surgery were as follows: before vitrectomy, 34.9°C; at the end of vitrectomy after plugging the sclerotomies and closing the infusion line for 5 min, 32.6°C [ 14 ]. This difference of 2.3°C may have been enough to decrease VEGF-A expression in the eye, as our present results revealed that a 2°C decrease caused a significant decrease in VEGF-A expression in vitro.…”

Section: Resultsmentioning

confidence: 99%

“…Romano et al measured and compared intraocular temperatures during surgery and found that the temperature within the anterior chamber was 23.6°C [ 13 ]. The retinal temperatures immediately adjacent to the fovea before and after vitrectomy were 34.9 and 32.6°C, respectively [ 14 ]. Retinal temperature just after vitrectomy is 2.3°C lower than before vitrectomy with the eyelid open, suggesting that the difference may be induced by cooling mediated by outside air and an anatomical alteration in the vitreous cavity after vitrectomy.…”

Section: Introductionmentioning

confidence: 99%

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Decreased VEGF-A and sustained PEDF expression in a human retinal pigment epithelium cell line cultured under hypothermia

et al. 2015

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BackgroundPrevious reports have described a decrease in retinal temperature and clinical improvement of wet age-related macular degeneration (AMD) after vitrectomy. We hypothesized that the retinal temperature decrease after vitrectomy plays a part in the suppression of wet AMD development. To test this hypothesis, we evaluated the temperature dependence of the expression of vascular endothelial growth factor-A (VEGF-A) and in vitro angiogenesis in retinal pigment epithelium (RPE).ResultsWe cultured ARPE-19 cells at 37, 35, 33 and 31°C and measured the expression of VEGF-A, VEGF-A splicing variants, and pigment epithelium–derived factor (PEDF). We performed an in vitro tube formation assay. The dehydrogenase activity was also evaluated at each temperature. Expression of VEGF-A significantly decreased with decreased temperature while PEDF expression did not. VEGF165 expression and in vitro angiogenesis also were temperature dependent. The dehydrogenase activity significantly decreased as the culture temperature decreased.ConclusionsRPE cultured under hypothermia that decreased cellular metabolism also had decreased VEGF-A and sustained PEDF expression, creating an anti-angiogenic environment. This mechanism may be associated with a beneficial effect after vitrectomy in patients with wet AMD.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Decreased VEGF-A and sustained PEDF expression in a human retinal pigment epithelium cell line cultured under hypothermia

et al. 2015

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“…After closing the infusion line, intraocular tissue rewarms rapidly [29, 30]. These temperature fluctuations could influence many biological functions like bleeding, expression of cytokines in the vitreous, induction of specific patterns of proteins, and neuroprotection.…”

Section: Discussionmentioning

confidence: 99%

Expression of VEGF-A, Otx Homeobox and p53 Family Genes in Proliferative Vitreoretinopathy

Azzolini

Pagani

Pirrone

et al. 2013

Mediators of Inflammation

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Introduction. Proliferative vitreoretinopathy (PVR) is a severe inflammatory complication of retinal detachment. Pathological epiretinal membranes grow on the retina surface leading to contraction, and surgery fails in 5% to 10% of the cases. We evaluated the expression of VEGF-A, Otx1, Otx2, Otx3, and p53 family members from PVR specimens to correlate their role in inducing or preventing the pathology. Methods. Twelve retinal samples were taken from patients affected by PVR during therapeutic retinectomies in vitreoretinal surgery. Gene expression was evaluated using quantitative real-time reverse transcriptase PCR analysis and immunohistochemistry, using four healthy human retinae as control. Result. Controls showed basal expression of all genes. PVR samples showed little or no expression of Otx1 and variable expression of VEGF-A, Otx2, Otx3, p53, and p63 genes. Significant correlation was found among VEGF-A, Otx2, p53, and p63 and between Otx1 and Otx3. Conclusions. Otx homeobox, p53 family, and VEGF-A genes are expressed in PVR human retina. We individuated two possible pathways (VEGF-A, Otx2, p53, p63 and Otx1 and Otx3) involved in PVR progression that could influence in different manners the course of the pathology. Individuating the genetic pathways of PVR represents a novel approach to PVR therapies.

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“…Previous studies have shown that the in vivo temperature of the vitreous cavity may range from 33.98C to 36.68C, with a likely temperature gradient existing. 41,42 For this reason, the T 1 relaxation times measured from the phantom porcine vitreous and BSS were temperature corrected to 358C to simulate, as accurately as possible, the temperature in the mid vitreous cavity. Similarly, when calibrating the T 1 times for either vitreous or aqueous, the assumption was made that the patients' mid vitreous cavity temperature at the time of their scans was 358C.…”

Section: Discussionmentioning

confidence: 99%

Measuring the Effect of Pars Plana Vitrectomy on Vitreous Oxygenation Using Magnetic Resonance Imaging

Simpson

Dowell

Jackson

et al. 2013

Invest. Ophthalmol. Vis. Sci.

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Determination of Retinal and Vitreous Temperature in Vitrectomy

Abstract: During routine vitreous surgery, the vitreous cavity and retina are cooled to much lower temperatures than those used in therapeutic hypothermia. Rapid rewarming occurs within the eye once the infusion line is closed.

Cited by 32 publications

References 6 publications

Decreased VEGF-A and sustained PEDF expression in a human retinal pigment epithelium cell line cultured under hypothermia

Decreased VEGF-A and sustained PEDF expression in a human retinal pigment epithelium cell line cultured under hypothermia

Expression of VEGF-A, Otx Homeobox and p53 Family Genes in Proliferative Vitreoretinopathy

Measuring the Effect of Pars Plana Vitrectomy on Vitreous Oxygenation Using Magnetic Resonance Imaging

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