Determination of riboflavin in urine and beverages by capillary electrophoresis with in-column optical fiber laser-induced fluorescence detection

Li, Hu; Yang, Xiupei; Yuan, Huatang; Xiao, Dingquan

doi:10.1016/j.jchromb.2007.06.011

Cited by 78 publications

(42 citation statements)

References 29 publications

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“…The concentrations of B2 in urine were at the maximum within 4 h after ingesting, whereas the concentration of B6 rapidly increased after 6 h. Then the concentration was decreased quickly after 8 h metabolism. The result was similar to the previous literature [7]. Therefore, the results can be accurate, and the proposed method can be used for the determination of vitamin B in complex samples.…”

Section: Application Of the Real Urinary Samplesupporting

confidence: 88%

“…There are a great variety of analytical methods based on spectrophotometry [3], fluorimetry [4], electrochemistry [5,6], capillary electrophoresis and chip electrophoresis [2,7], HPLC [8,9] and mass spectrometry [10] monitoring the overall quantification of the given vitamin B samples. However, some of these methods only offer the possibility of individual or two quantifications of the given analytes.…”

Section: Introductionmentioning

confidence: 99%

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Assay of vitamin B in urine by capillary electrochromatography with methacrylate‐based monolithic column

Wei

Qiao

et al. 2010

Electrophoresis

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A novel and simple method for the separation of major vitamin B analytes, such as thiamine, riboflavin, nicotinamide, vitamin B4, pyridoxine, has been developed by CEC using the monolithic column. It has been found that the baseline separation of the five analytes could be achieved with 5.0 mM phosphate buffer at pH 4.0. Compared with the open-tubular capillary and the bared capillary columns, the poly(butylmethacrylate-co-ethylene glycol dimethacrylate) monolithic capillary could exhibit the best resolution in the analysis. Then the method was validated and the linear calibration ranges were obtained with correlation coefficients more than 0.997. The precision and the recovery were also investigated and showed a good result. Furthermore, the proposed method was successfully applied to assay the concentration of vitamin B analytes and the metabolic situation in human urine samples.

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Section: Application Of the Real Urinary Samplesupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Assay of vitamin B in urine by capillary electrochromatography with methacrylate‐based monolithic column

Wei

Qiao

et al. 2010

Electrophoresis

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“…As previously reported [11,12], RF in aqueous medium presents two excitation maxima at 8 approximately 368 and 440 nm, and a fluorescence emission maximum at 524 nm. The pH changes in the range 4-8 did not produce any significant modifications in the fluorescence signal [33].…”

Section: Preliminary Studiessupporting

confidence: 80%

“…When RF intake is low, excretion is proportional to the intake [3]. The most widely used analytical methods for the quantification of RF in human urine samples are liquid chromatography (LC)-fluorescence detection (FD) [8], LC-tandem mass spectrometry (MS/MS) [9,10], fluorescence with flow-injection analysis [11], capillary electrophoresis (CE) [12,13], voltammetry [14] and chemiluminescence [15]. These methods require rigorous extraction steps, the use of significant amounts of organic solvents and long analysis time.…”

Section: Introductionmentioning

confidence: 99%

Development of a second-order standard addition fluorescence method for the direct determination of riboflavin in human urine samples without previous clean up and separation steps

Privitera

Lozano

2017

Microchemical Journal

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A new strategy for the determination of riboflavin (RF, or vitamin B2) in human urine samples has been developed, combining excitation-emission fluorescence matrix (EEFM) data in standard addition mode and second-order chemometric analysis. The method is simple, fast and eco-friendly because it complies with the green analytical chemistry principles, avoiding the need of previous clean up and separation steps that consume high amounts of organic solvents. Successful results were obtained by different chemometric algorithms, namely parallel factor analysis (PARAFAC), unfolded partial leastsquares/residual bilinearization (U-PLS/RBL) and multidimensional partial leastsquares/residual bilinearization (N-PLS/RBL), all in the modified standard addition. These algorithms allowed us to achieve selectivity in a system, which requires standard addition and shows a significant background spectral overlapping with the studied vitamin. The quality of the proposed strategy was evidenced on the basis of the analytical recoveries from urine samples spiked with RF. The detection limits achieved in urine samples are encouraging compared to those obtained using chromatographic approaches. The relative prediction errors were lower than 5.6 %.

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“…Recently, our research group has successfully developed in-column optical fiber induced-fluorescence detection system for capillary electrophoresis (CE) [16][17][18][19][20][21][22][23][24][25]. For the in-column optical fiber induced-fluorescence detection system, our previous work demonstrated that an excitation light beam waveguided by an optical fiber to the detection point of the capillary shows relatively lower background light than that obtained from an excitation light beam impinged orthogonally on the capillary exterior wall.…”

Section: Introductionmentioning

confidence: 99%

HPLC with In-Capillary Optical Fiber Laser-Induced Fluorescence Detection of Picomolar Amounts of Amino Acids by Precolumn Fluorescence Derivatization with Fluorescein Isothiocyanate

Zhao

Yuan

et al. 2011

Chromatographia

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In this paper, a fluorescein isothiocyanate (FITC) precolumn derivatization technique in conjunction with an HPLC-in-capillary optical fiber laser-induced fluorescence (HPLC-ICOF-LIF) detection method has been developed for determination of amino acids. The HPLC separation of FITC-labeled amino acids and the ICOF-LIF detection system are studied and optimized. Optimum separation conditions were obtained with a gradient elution program of acetonitrile and phosphate buffer (10 mM, pH 6.8). The ICOF-LIF detection system comprises a 530-lm capillary and a 380-lm optical fiber. The analyses of amino acids display excellent linear relationship between peak area and concentration with correlation coefficients greater than 0.999 and the method also provides good repeatability with RSD \ 3%. The detection limits for FITC-tagged amino acids are very low and the lowest LOD for tyrosine is 51 pM. The proposed method has been successfully applied to determination of amino acids in human serum. Our developed HPLC-ICOF-LIF system is cheap, simple, stable, and sensitive which is potentially useful for the formulation analysis and bioanalysis.

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Determination of riboflavin in urine and beverages by capillary electrophoresis with in-column optical fiber laser-induced fluorescence detection

Cited by 78 publications

References 29 publications

Assay of vitamin B in urine by capillary electrochromatography with methacrylate‐based monolithic column

Assay of vitamin B in urine by capillary electrochromatography with methacrylate‐based monolithic column

Development of a second-order standard addition fluorescence method for the direct determination of riboflavin in human urine samples without previous clean up and separation steps

HPLC with In-Capillary Optical Fiber Laser-Induced Fluorescence Detection of Picomolar Amounts of Amino Acids by Precolumn Fluorescence Derivatization with Fluorescein Isothiocyanate

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