Determination of rivaroxaban in rat plasma by ultra‐high‐performance liquid chromatography–Q‐Orbitrap high‐resolution mass spectrometry and its application to a pharmacokinetic study

Jiang, Qian; Gu, Shun-Zhong; Yan, Yongjin

doi:10.1002/bmc.5491

Cited by 3 publications

(1 citation statement)

References 29 publications

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“…A hybrid QqQ linear ion‐trap is a less employed mass analyzer (Tanner et al, 2019). To date, LC‐HRMS is starting to be used for the pharmacokinetic studies of small molecules, for example, LC coupled with quadrupole in tandem with Orbitrap (Alkhateeb et al, 2020; Park et al, 2021; Qian et al, 2022; Wang, Wang, et al, 2019). The number of publications on this topic has been rising every year.…”

Section: Ms‐based Methods In the Analysis Of Small‐molecule Chemical ...mentioning

confidence: 99%

What is the role of current mass spectrometry in pharmaceutical analysis?

Khalikova

Jireš

Horáček

et al. 2023

Mass Spectrometry Reviews

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The role of mass spectrometry (MS) has become more important in most application domains in recent years. Pharmaceutical analysis is specific due to its stringent regulation procedures, the need for good laboratory/manufacturing practices, and a large number of routine quality control analyses to be carried out. The role of MS is, therefore, very different throughout the whole drug development cycle. While it dominates within the drug discovery and development phase, in routine quality control, the role of MS is minor and indispensable only for selected applications. Moreover, its role is very different in the case of analysis of small molecule pharmaceuticals and biopharmaceuticals. Our review explains the role of current MS in the analysis of both small‐molecule chemical drugs and biopharmaceuticals. Important features of MS‐based technologies being implemented, method requirements, and related challenges are discussed. The differences in analytical procedures for small molecule pharmaceuticals and biopharmaceuticals are pointed out. While a single method or a small set of methods is usually sufficient for quality control in the case of small molecule pharmaceuticals and MS is often not indispensable, a large panel of methods including extensive use of MS must be used for quality control of biopharmaceuticals. Finally, expected development and future trends are outlined.

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Section: Ms‐based Methods In the Analysis Of Small‐molecule Chemical ...mentioning

confidence: 99%

What is the role of current mass spectrometry in pharmaceutical analysis?

Khalikova

Jireš

Horáček

et al. 2023

Mass Spectrometry Reviews

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A sustainable and green HPLC-PDA technique for the simultaneous estimation of Post-COVID-19 syndrome co-administered drugs with greenness and whiteness assessment

Medhat,

Fouad,

Monir

et al. 2024

Sci Rep

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COVID-19 has been a growing global concern in the past four years. Several syndromes associated with this multi-organ viral infection have been observed since the outbreak. According to estimates, 10–15% of people with SARS-CoV- infection may have post-COVID-19 syndrome. Even months after infection, common residual signs and symptoms include myalgia, exhaustion, shortness of breath, rapid heartbeat, stroke, and memory and cognitive impairment which can negatively affect survivors’ quality of life and may consequently lead to their death. Therefore, it is necessary to think about potential therapy options for dealing with both short and long-term impacts. Paracetamol (a common analgesic and antipyretic) and Dexketoprofen Trometamol (a non-steroidal anti-inflammatory drug) are used together to relieve post-COVID symptoms like myalgia (muscle pain) and headache. Additionally, to prevent thrombotic events, Rivaroxaban is recommended for 35 days following discharge. Thus an eco-friendly HPLC-DAD technique was developed for simultaneous quantification of Paracetamol, Dexketoprofen Trometamol, and Rivaroxaban which are co-administered for treatment of post-COVID-19 syndrome. The suggested method was found to be linear in the concentration ranges of 3.00–45.00 µg/mL, 0.5–50.00 µg/mL, and 0.15–20.00 µg/mL, and a limit of detection down to 0.531 µg/mL, 0.095 µg/mL and 0.047 µg/mL for Paracetamol, Dexketoprofen Trometamol and Rivaroxaban, respectively. This method was effectively used to quantify the studied drugs in their bulk powder and spiked human plasma with high percentage recoveries (96.55–99.46%). The suggested approach was validated per International Conference on Harmonization (ICH) requirements and found to be within the acceptable ranges. The method was developed using Green Analytical Chemistry (GAC) principles, with the solvents used and run time having a significant effect on the method’s greenness. “Non-toxic” ethanol served as the organic modifier in the mobile phase, moreover, the total run time was 12 min making it suitable for the routine analysis of the mentioned drugs in plasma samples. To get a full image of the method’s greenness profile; two most recent greenness assessment tools, the Green Analytical Procedure Index (GAPI), and the Analytical GREEnness metric (AGREE), were employed, with White Analytical Chemistry (WAC) principles proving its environmental safety.

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An Eco-Friendly Ultra-Performance Liquid Chromatography–Mass Spectrometry Method for Quantification of Rivaroxaban and Ticagrelor in Rat Plasma: Grapefruit Interactions

Moffid,

Mostafa,

Mahmoud

et al. 2023

Bioanalysis

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Aim: An eco-friendly ultra-performance liquid chromatography–tandem mass spectrometry method was developed to study the pharmacokinetics of rivaroxaban and ticagrelor in rat plasma, utilizing moxifloxacin as an internal standard. The food–drug interaction between grapefruit juice and these drugs was also investigated. Methods: Liquid–liquid extraction was used. A nonporous stationary phase Agilent® Poroshell 120EC C18 column was used with methanol: 0.1% aqueous formic acid (95:5 v/v) as a mobile phase. The detection was performed in multiple reaction monitoring mode using positive electrospray ionization. The method’s validation was conducted in accordance with US FDA and European Medicines Agency guidelines. Results & conclusion: Grapefruit juice should be ingested with caution in patients treated with antithrombotic medications as it may increase their plasma concentration, inducing bleeding, and requires close clinical monitoring.

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Determination of rivaroxaban in rat plasma by ultra‐high‐performance liquid chromatography–Q‐Orbitrap high‐resolution mass spectrometry and its application to a pharmacokinetic study

Cited by 3 publications

References 29 publications

What is the role of current mass spectrometry in pharmaceutical analysis?

What is the role of current mass spectrometry in pharmaceutical analysis?

A sustainable and green HPLC-PDA technique for the simultaneous estimation of Post-COVID-19 syndrome co-administered drugs with greenness and whiteness assessment

An Eco-Friendly Ultra-Performance Liquid Chromatography–Mass Spectrometry Method for Quantification of Rivaroxaban and Ticagrelor in Rat Plasma: Grapefruit Interactions

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