Determination of rosiglitazone and metformin in human serum by CE‐ESI‐MS

Abstract

A new method for the determination of anti-diabetic drugs metformin and rosiglitazone based on the use of capillary electrophoresis with electrospray mass spectrometry was developed. The proposed method allowed their separation within 11 min by using 50 mM formic acid at +20 kV. Positive electrospray ionization and selected ion monitoring [M+H](+) of metformin (m/z=130) and rosiglitazone (m/z=358) were performed. Several important experimental parameters influencing electrospray ionization of metformin and rosiglitazone were studied. The final composition of sheath liquid was water/methanol/formic acid (50:49.5:0.5, v/v/v), at a flow rate of 2 μL/min. The developed method was applied for the determination of metformin and rosiglitazone simultaneously in human serum after protein precipitation with acetonitrile. The limits of detection of developed method were 4.42 and 2.14 ng/mL for rosiglitazone and for metformin, respectively, which is sufficient for therapeutic serum concentration levels monitoring for both studied drugs.

LC–MS/MS method for the determination of rosiglitazone on rat dried blood spots and rat urine: Application to pharmacokinetics

“…Table 4. The 18 results were found to be well within the acceptance limits. 1 and 50% hematocrit were tested at 100 ng/mL of rosiglitazone.…”

“…Now it is successfully adopted and 17 widely practiced by clinical labs in newborn screening for inherited metabolic disorders. It 18 requires a micro volume (typically ≤50 μL) blood sample for drug quantification. Recently, it 19 has gained interest in drug discovery and development processes due to the fact that it offers 20 several advantages over conventional whole blood, plasma or serum sample collection 21 [30,31].…”

“…The use of animals was approved 17 by the 'Institutional Animal Ethical Committee' (169/99/CPCSEA, University College of 18 Pharmaceutical Sciences, Warangal, India). Throughout the experimental period, the animals 19 were housed under standard condition in cages at room temperature (20 ± 2 °C); relative 20 humidity (60 -70 %) and were exposed to 12/12 h light/dark cycle.…”

“…The intra-day precision expressed as relative standard deviation (RSD %) and the 16 accuracy expressed as relative error (RE %), were evaluated by analyzing six different QC 17 samples (n=6) at each of the low (0.15 ng/mL), medium (30 ng/mL) and high (80 ng/mL) 18 concentrations of the analyte on the same day. The inter-day precision and accuracy was 19 assessed by analyzing six batches of all QC samples on three different days.…”

“…urine (0.2 mL) was made up to 1 mL with water and loaded on to the solid phase 17 extraction (SPE) cartridges (ODS-SPE, 100 mg/1mL, Agela technologies) which had18 previously been washed with 2×1mL of methanol and 2×1mL of water. After washing with 2 19 mL of water, the drug was eluted with 2×1mL of methanol in a clean tube.…”