Determination of roxatidine in human plasma, urine and milk by capillary gas chromatography using nitrogen-selective detection

Quantification of roxatidine in human plasma by liquid chromatography electrospray ionization tandem mass spectrometry: Application to a bioequivalence study

“…Early publications have described methods of analyzing roxatidine in biological samples. The techniques adopted include gas chromatography-mass spectrometry (GC-MS) [3], high-performance liquid chromatography with an UV detector (HPLC-UVD) [4], and capillary gas chromatography with nitrogen selective detection [5]. The latest assay using liquid chromatography-mass spectrometry (LC-MS) was reported [6].…”

“…The latest assay using liquid chromatography-mass spectrometry (LC-MS) was reported [6]. However, these methods suffer from a number of disadvantages, e.g., they are time-consuming and require complex sample preparation [3], they need a large volume of plasma (≥0.1 mL) [4][5][6], have long run times (>4 min) [3][4][5][6], or have poor * Corresponding author. Tel.…”

“…E-mail address: ktlee@khu.ac.kr (K.-T. Lee). sensitivities [3][4][5][6]. Moreover, to the best of our knowledge, no liquid chromatography-tandem mass spectrometry (LC-MS/MS) based method has been previously described in the literature for the detection of roxatidine.…”

Determination of roxatidine in human plasma by liquid chromatography/electrospray mass spectrometry and application to a clinical pharmacokinetic study

“…The parent compound and roxatidine acetate are not detectable in biological fluids. Thus, all pharmacokinetic studies are performed by determining roxatidine levels in biological fluids 5–8. Roxatidine, the primary metabolite of roxatidine acetate, possesses a potent H 2 ‐receptor antagonist activity.…”

“…A number of assay methods have been reported for the determination of roxatidine in biological fluids, including gas chromatography/mass spectrometry (GC/MS),5 GC using a nitrogen‐selective detector,6 and high‐performance liquid chromatography ultraviolet detection (HPLC‐UVD) 10. Lately, liquid chromatography/mass spectrometry (LC/MS) and tandem mass spectrometry (LC/MS/MS) have been increasingly utilized as they can analyze test samples regardless of their purity in biological substances and measure blood concentrations with high sensitivity and selectivity.…”