Determination of salbutamol in human plasma and urine by high-performance thin-layer chromatography

Colthup, P V; Dallas, F.A.A.; Saynor, D. A.; Carey, P.F.; Skidmore, L.F.; Martin, Lee; Wilson, Keith A.

doi:10.1016/0378-4347(85)80140-7

Cited by 26 publications

(6 citation statements)

References 11 publications

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“…With regards to the detection of salbutamol, several techniques have also been described in the literature, e.g. high performance thin layer chromotography (HPTLC) (Colthup et al 1985), radio-immunoassay (RIA) (Loo et al 1987), high performance liquid chromatography (HPLC) with electrochemical (Oosterhuis and van Boxtel 1982, Tan and Soldin, 1984, Emmet al 1988, Sagar et al 1993 or fluorescence detection (Hutchings et al 1983, Miller and Greenblatt 1986, McCarthy et al 1993, or gas chromatography coupled to mass spectrometry (GC-MS) in the electron impact (El) mode (Martin et al 1976) or under positive chemical ionization (PCI) using ammonia (Leferink etal. 1982, WeisenbergereiaZ.…”

Section: Introductionmentioning

confidence: 99%

Analysis ofβ‐agonists in urine and tissues by capillary gas chromatography‐mass spectrometry:In vivostudy of salbutamol disposition in calves

Montrade¹,

Bizec²,

Monteau³

et al. 1995

Food Additives and Contaminants

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The fate of salbutamol sulphate given orally has been investigated in calves. The urinary excretion rate and the tissue distribution of this beta-agonistic drug were studied by capillary gas chromatography coupled to low resolution mass spectrometry (GC-LRMS) under electron impact (EI) ionization mode, using an hexadeuterated salbutamol analogue as the internal standard. The parent drug and metabolites were extracted via solid phase extraction (SPE) mixed-phase-containing disposable columns and analysed as their trimethylsilyl derivatives. A more efficient clean-up had to be carried out for tissue samples. An acidic precipitation followed by a liquid-liquid extraction were therefore performed before the SPE. Moreover, the problem of tissue digestion was elucidated by means of an ultrasonic probe. Samples were also analysed before and after enzymic hydrolysis using purified beta-glucuronidase and a mixture of beta-glucuronidase and arylsulphatase, to obtain evidence of phase II conjugation mechanisms. Both free salbutamol and conjugated metabolites were detected in urine and tissue samples. Except for liver or kidney, salbutamol was rapidly cleared from most tissues after a withdrawal period. The possible excretion of some phase I metabolites was also investigated, using further analyses under positive chemical ionization LRMS and high resolution mass spectrometry (HRMS).

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Section: Introductionmentioning

confidence: 99%

Analysis ofβ‐agonists in urine and tissues by capillary gas chromatography‐mass spectrometry:In vivostudy of salbutamol disposition in calves

Montrade¹,

Bizec²,

Monteau³

et al. 1995

Food Additives and Contaminants

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show abstract

“…Several analytical techniques have been reported for the determination of salbutamol in its pharmaceutical preparations including chromatographic [2][3][4], fluorescent [5], electrochemical [6,7], a.c.oscillopolarographic titration [8] and flow injection [9] methods. The British Pharmacopoeia [10] involves two different methods for the analysis of pure drug of salbutamol and its formulations.…”

Section: (I)mentioning

confidence: 99%

Development of Spectrophotometric methods for Assay of Salbutamol in Pharmaceutical Formulations

Al-Sabha¹

2007

J.Edu.Sci.

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Two sensitive spectrophotometric methods are proposed for the determination of salbutamol in pure form and pharmaceutical preparations. The methods are based on the oxidation of salbutamol drug with Fe(III) in acidic medium. The liberated Fe(II) reacts with 1,10phenanthroline in method A and the ferroin complex is colorimetrically measured at 510 nm against reagent blank. Method B is based on the reaction of the liberated Fe(II) with 2,2'-bipyridyl to form a stable coloured complex with maximum absorption at 520 nm against reagent blank. Beer's law was obeyed in the concentration range of 0.15-6.00 µg/ml and 0.25-8.00µg/ml with molar absorptivity of 7.858×10 4 and Development of Spectrophotometric methods for …… 26 4.555×10 4 l.mol -1 .cm -1 for method A and B respectively. The mean percent recoveries are 100.39% and 99.11% for both methods respectively. The suggested procedures could be used for the determination of salbutamol in pure and dosage forms without interference from common excipients. The proposed methods are successfully compared with the official and other spectrophotometric methods.

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“…In addition assay of drugs taken by the inhaled route is problematical because of the very small doses and the small proportion of drug which is actually retained within the body. Nonetheless using a method based on a high performance liquid chromatography (HPLC) assay of CoLnnJP et al [9], HoRN et al [2] have documented that salbutamol will be detectable in urine for at least four hours after inhalation of 200 jlg of salbutamol. To date this is the only system suitable for large studies of compliance with inhaled therapy.…”

Section: Cr Hornmentioning

confidence: 99%

The assessment of therapeutic compliance by asthmatic patients

Horn¹

1992

Eur Respir J

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Determination of salbutamol in human plasma and urine by high-performance thin-layer chromatography

Cited by 26 publications

References 11 publications

Analysis ofβ‐agonists in urine and tissues by capillary gas chromatography‐mass spectrometry:In vivostudy of salbutamol disposition in calves

Analysis ofβ‐agonists in urine and tissues by capillary gas chromatography‐mass spectrometry:In vivostudy of salbutamol disposition in calves

Development of Spectrophotometric methods for Assay of Salbutamol in Pharmaceutical Formulations

The assessment of therapeutic compliance by asthmatic patients

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