Determination of Salbutamol in Human Plasma and Urine by High-Performance Liquid Chromatography with a Coulometric Electrode Array System

Abstract

A method is developed to determine salbutamol in human plasma and urine using high-performance liquid chromatography (HPLC) with a coulometric electrode array system, based on the electrochemical behavior of salbutamol at graphite electrode. The mobile phase component A is 30 mM sodium dihydroxy phosphate-30 mM triethylamine and is adjusted to pH 6.0 with 20% phosphate acid. The mobile phase component B is methanol. The optimized mobile phase composition was A and B in the proportion of 90:10 (v/v). Paracetamol is selected as the external standard. The human plasma and urine samples are pretreated using solid-phase extraction cartridges (Sep-Pak Silica), and the eluting solution is monitored by the coulometric electrode array system. The electrode potentials are set at 300, 400, 550, and 650 mV, respectively. Calibration curves show good linearity, and the recovery of salbutamol proves to be constant and unaffected by the concentration of the drug. This method, developed using HPLC-electrochemical detection, is reproducible and sensitive enough for the determination of salbutamol in human plasma and urine.

Synthesis of Mesoporous Silica Imprinted Salbutamol with Two TEOS/MTES Ratio Compositions through the Direct Incorporation Method for Salbutamol Separation

“…The SPE using the Sep-Pak Silica column is used as a pretreatment of samples. The recovery of this method reached about 77.32% and 81.25% [ 53 ]. Compared with the recovery results in this study, MIPMS-SPE can increase the value of % recovery of salbutamol compared to conventional SPE.…”

Electrochemical and Hyphenated Electrochemical Detectors in Liquid Chromatography and Flow Injection Systems for Drug Compound Analysis

“…Tables 7.2 and 7.3 show the LC-EC and FIA-EC with their hyphenated EC-MS detection systems used in the methods, respectively. Plasma sample [74] (continued) Cerebrospinal fluid [83] (continued) (continued) [107] (continued) Bovine and human serum samples [118] (continued) 7.5 Hyphenated Techniques for LC and FIA Phyto-pharmaceutical formulations [130] (continued) Plasma and urine samples [144] (continued) …”

A Study of Retention Behavior and Method Development of Salbutamol Sulfate in Hydrophilic Interaction Chromatography

“…4,5 Nevertheless, there is no literary research in this field of estimating and separating SBS using ZIC-hydrophilic (ZIC-HILIC) techniques, despite a number of studies for the separation and determination of SBS in High-performance RESEARCH ARTICLE liquid chromatography (HPLC). [6][7][8][9] In addition, the effect of the ZIC-HILIC column's chain longitude on SBS retention behavior was not previously studied. In a recent study, Rasheed and coworkers [10][11][12] studied the effect of chain length on HILIC columns and its effect on ranitidine and famotidine behaviors.…”