Determination of secondary metabolites and antioxidant activity of some boraginaceae species growing in Iran

Gharib, A E Fatma; Godarzee, Masoud

doi:10.4314/tjpr.v15i11.22

Cited by 18 publications

(6 citation statements)

References 20 publications

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“…These results are explained by the difference in the mechanism of the radical interaction of these two assays due to the fact that ABTS acts as both a hydrophobic and hydrophilic system, while DPPH acts only as a hydrophobic one. A connection between antioxidant activity and phenolic content has been already established for plants of the Boraginaceae family [36]. It is speculated that much of the family's biological effects (i.e., anti-inflammatory and antioxidant) are relative to the concentration of phenolic compounds, which are abundant in Boraginaceae plants [37].…”

Section: Total Phenolic/flavonoid Content and Antioxidant Activity Rementioning

confidence: 99%

Rindera graeca (Boraginaceae) Phytochemical Profile and Biological Activities

et al. 2020

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Rindera graeca is a Greek endemic plant of the Boraginaceae family which has never been studied before. Consequently, this study attempted to phytochemically examine the aerial parts of this species. Nine phenolic secondary metabolites were identified, consisting of seven caffeic acid derivatives and two flavonol glucosides, namely rutin and quercetin-3-rutinoside-7-rhamnoside. These flavonoids, together with rosmarinic acid, were isolated via column chromatography and structurally determined through spectral analysis. Quercetin-3-rutinoside-7-rhamnoside is an unusual triglycoside, which is identified for the first time in Rindera genus and among Boraginaceae plants. This metabolite was further examined with thermal analysis and its 3D structure was simulated, revealing some intriguing information on its interaction with biological membrane models, which might have potential applications in microcirculation-related conditions. R. graeca was also analyzed for its pyrrolizidine alkaloids content, and it was found to contain echinatine together with echinatine N-oxide and rinderine N-oxide. Additionally, the total phenolic and flavonoid contents of R. graeca methanol extract were determined, along with free radical inhibition assays. High total phenolic content and almost complete inhibition at experimental doses at the free radical assays indicate a potent antioxidant profile for this plant. Overall, through phytochemical analysis and biological activity assays, insight was gained on an endemic Greek species of the little-studied Rindera genus, while its potential for further applications has been assessed.

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Section: Total Phenolic/flavonoid Content and Antioxidant Activity Rementioning

confidence: 99%

Rindera graeca (Boraginaceae) Phytochemical Profile and Biological Activities

et al. 2020

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“…e results of this study show that not all polyphenols in crude extracts are necessarily flavonoids but may be tannins or phenolic acids. Many species of this genus have been studied and reported on previously, including N. caspica [35], N. micrantha [36], and N. lutea [37]. Compared to the current study, they had greater amounts of polyphenols and flavonoids.…”

Section: Total Phenolic and Flavonoidmentioning

confidence: 50%

Microwave-Assisted Extraction and Phytochemical Profile of Nonea pulmonarioides and Its Antifungal, Antibacterial, and Antioxidant Activities

Mohammed

Abdullah

2022

Journal of Food Quality

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Microwave-assisted extraction (MAE) was chosen to isolate secondary natural compounds from Nonea pulmonarioides. MAE is an efficient approach compared to maceration. In our study of N. pulmonarioides, the Folin–Ciocalteu and trichloroaluminum techniques were used to determine the total phenolic and flavonoid contents. Antioxidant activity was determined by the β-carotene/linoleic acid test. The minimum inhibitory concentration (MIC) was measured using the microwell dilution assay method. Overall, MAE may represent a substantially faster extraction system (5 min) with an even greater extraction yield than maceration extraction (24 h). Phytochemical screening indicated the existence of classes of several secondary metabolites. The methanol extract had a high total phenolic content (TPC) of 113.33 ± 1.06 μg of gallic acid equivalents (GAE)/mg of dry plant weight, while the total flavonoid content (TFC) in the acetone extract was 44.26 ± 0.88 μg of quercetin equivalent (QE)/mg of the dry plant. The antioxidant activity data correlated well with the acetone extract (32.41 ± 0.93, I%) compared to the other extracts. The antimicrobial assay (MIC) results showed high activity of the extract against Candida albicans for the petroleum ether extract, which was significantly higher than against other microorganisms. The quantitative gas chromatography-mass spectrophotometry (GC-MS) analysis of the extracts confirmed that the most abundant compound in the methanol extract was ethyl tridecanoate (45.94%), while nonadecane was the predominant compound in the acetone (35.7%) and the petroleum ether (18.34%) extracts.

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“…Thus far, only few studies have described the phyto-chemical analysis of plants belonging to the genus Onosma . Species of this genus contain secondary metabolites such as naphthoquinone (Vukic et al, 2017 ), phenolic andflavonoid compounds (Gharib et al, 2016 ; Ozer et al, 2017; Khaledi et al, 2018 ), and pyrrolizidine alkaloids (Damianakos et al, 2013 ; El-shazly et al, 2014 ), which exhibit antioxidant and antimicrobial activities (Li et al, 2010 ; Kumar et al, 2020 ). Phytochemical investigation of the genus Onosma led to the discovery of a series of shikonin and alkannin derivatives (Kretschmer et al, 2012 ) as compounds that exhibit antimicrobial activity (Vukic et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

“…The total flavonoid contents were determined by a previously described method (Gharib et al, 2016 ) by using quercetin as a reference compound. Briefly, 0.5 ml of each extract (0.1 g/ml) was mixed with 1.5 ml of methanol, 0.1 ml of aluminum chloride (10%), 0.1 ml of sodium acetate (1M), and 2.8 ml of distilled water.…”

Section: Methodsmentioning

confidence: 99%

“…Phytochemical investigation of the roots of Onosma led to the discovery of a series of shikonin and alkannin derivatives (Kretschmer et al, 2012 ) as compounds with a wide spectrum of biological properties such as wound healing (Madanid and Shokoohiniac, 2017 ), anti-inflammatory and anti-oxidant (Kumar et al, 2017 ), anti tumor (Hasenoehrl et al, 2017 ), antiviral (Chen et al, 2003 ), and antibacterial and antifungal properties (Ahmad et al, 2009 ; Zarghami Moghaddam et al, 2012 ). The aerial parts of the Onosma plant contain pyrrolizidine alkaloids (Damianakos et al, 2013 ; El-shazly et al, 2014 ) and phenolic compounds (Gharib et al, 2016 ; Ozer et al, 2017; Khaledi et al, 2018 ). Additionally, species of this genus contain secondary metabolites such as alkaloids, naphthoquinones, polyphenols, phytosterols, and fatty acids, which exhibit antioxidant and antimicrobial activities (Kumar et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of the phenolic contents and antibacterial activity of different concentrations of Onosma chlorotricum Boiss.

Dousti¹,

Nabipor²

2021

bta

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Because of side effects and increasing resistance to antibiotics, considerable attention hasbeen given to extract sand biologically active compounds isolated from medicinal plants. In the present study, phytochemical screening and antibacterial activities of different concentrations of various extracts of Onosma chlorotricum were evaluated and compared with those of vancomycin and amikacin. Total phenolic and flavonoid contents and the antibacterial activity of methanol, n-hexane, and aqueous extracts (5 mg/ml to 0.156 mg/ml final concentration) of O. chlorotricum against Staphylococcus aureus, Pseudomonas aeruginosa , and Escherichia coli were evaluated using disc diffusion and microdilution methods. Total phenolic and flavonoid contents were determined according to the Folin-Ciocalteu and aluminum chloride colorimetric assays, respectively. The results showed that the total phenolic and flavonoid contents of these extracts ranged from 56.10 ± 0.13 to 74.12 ± 0.05 mg GAE/g dry extract and from 19.3 ± 0.6 to 23.20 ± 0.41 mg QE/g dry extract, respectively. The methanol extract with the highest phenolic and flavonoid content showed the highest antibacterial activity against all the tested bacterial strains, with the highest inhibition zone of 21 ± 0.7 mm and the lowest MIC and MBC values of 78.12 μg/ml for S. aureus . The antibacterial effects and the total phenolic content of O. chlorotricum were remarkable. The results suggest that the effects of methanol extract on wound healing could be tested in an animal model.

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Determination of secondary metabolites and antioxidant activity of some boraginaceae species growing in Iran

Cited by 18 publications

References 20 publications

Rindera graeca (Boraginaceae) Phytochemical Profile and Biological Activities

Rindera graeca (Boraginaceae) Phytochemical Profile and Biological Activities

Microwave-Assisted Extraction and Phytochemical Profile of Nonea pulmonarioides and Its Antifungal, Antibacterial, and Antioxidant Activities

Evaluation of the phenolic contents and antibacterial activity of different concentrations of Onosma chlorotricum Boiss.

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