Determination of selenium in blood serum by inductively coupled plasma atomic emission spectrometry with pneumatic nebulization

Abstract

The possibility of determining selenium in blood serum using inductively coupled plasma emission spectrometry with conventional pneumatic nebulization was studied. A high-resolution spectrometer (SBW=6 pm) with laterally viewed ICP was employed. Analysis with conventional pneumatic nebulization could overcome laborious and demanding digestion, which is necessary for hydride generation. A pressure digestion with nitric acid at 160 degrees C was sufficient to decrease the carbon content in the serum sample to 5%-10% of its original value. Spectral interference of the CN band was observed and mathematically corrected. It was found that the carbon-induced selenium line emission enhancement occurred even under ICP optimized conditions. A method of determination was developed and applied to the analysis of blood serum. True limit of detection in real samples is 0.01-0.02 mg/L and the limit of quantification (RSD 10%) is 0.03-0.07 mg/L using Se I 196.090 nm line at an integration time of 10-2 s. The method was tested by analysis of porcine blood serum and the serum reference material Seronorm MI 0181.

Effects of Inclusion of Selenium-Enriched Yeast in the Diet of Laying Hens on Performance, Eggshell Quality, and Selenium Tissue Deposition

“…Two sets of blood samples were collected before slaughtering from 8 animals per group from the jugular vein, put into two 10-mL vacuum tubes (Venojet VT050SP Terumo, Japan) and subsequently centrifuged (1400 ¥ g ¥ 10 min). Two serum aliquots from each tube were than collected and frozen (-20°C) until analysis for Se (Machát et al, 2002), glucose (Centers for Disease Control, 1976), total protein (Hiller et al, 1948), albumin (Doumas et al, 1971), cholesterol (Allain et al, 1974), alanine transaminase (ALAT) and aspartate transaminase (ASAT) (Henry et al, 1960), bilirubin (Mori, 1978), alkaline phosphatase (ALP) content (Browers and Comb, 1966), and serum glutathione peroxidase activity (GSHPx) (Paglia and Valentine, 1967).…”

Rapid and simple determination of selenium in blood serum by inductively coupled plasma–mass spectrometry (ICP–MS)

“…For ten years inductively coupled plasma mass spectrometry (ICP-MS) has been regarded as an important technique explored for its potential for selenium analysis [6,7]. Selenium is not as sensitive as other elements owing to its high ionization potential.…”

“…However, developments in ICP-MS have shown that it is possible to detect selenium in biological materials and in water or soil with greater sensitivity than by AAS or ICP-AES [8,9]. ICP-MS is a powerful technique for trace elemental analysis [6,7]. But, spectral interferences with limited sensitivity have been improved with ICP-MS coupled with hydride generation.…”

“…Machat et al [7] described a new analytical procedure for the selenium determination in blood serum with inductively coupled plasma atomic emission spectrometry (ICP-AES). The authors verified the applicability of an ICP-AES instrument with a high-resolution monochromator without hydride generation.…”

“…The authors verified the applicability of an ICP-AES instrument with a high-resolution monochromator without hydride generation. It was shown that determination of selenium in blood serum with conventional pneumatic nebulization should overcome a complicated digestion technique, which is required for hydride generation [7].…”