Determination of selenium species in plant leaves by HPLC–UV–HG-AFS

Selenium and Its Species Distribution in Above-ground Plant Parts of Selenium Enriched Buckwheat (Fagopyrum esculentum Moench)

“…The operating UV conditions for both instruments are presented in Table 1, and detailed descriptions for both methods were published elsewhere. 19,22 The described separation conditions enabled the determination of Se(IV), Se(VI), SeMet, SeMeSeCys and SeCys2 with both anion and cation columns. Standards containing each Se species with a concentration of about 100 ng Se g -1 , except for SeMet at 300 ng Se g -1 , were prepared in Milli-Q water.…”

“…Therefore, special care concerning sample extraction and the storage time of the extracts should be taken. determination of Se-species in plant samples the most frequently used methods are HPLC combined with UV-HG-AFS (with UV treatment) 22 and HPLC in combination with ICP-MS (inductively coupled plasma mass spectrometry), which has a lower detection limit in comparison to HPLC-UV-HG-AFS. 15,23 However, the latter was primarily used in our study because it is simple, more rapid and less costly, and therefore more appropriate for routine work, particularly since Se-enriched plants were analyzed.…”

Selenium and plants

“…in the form of selenium added. Beside inorganic Se, they also found SeMet (4.2-8.4%) and SeMeSeCys (<DL−0.7%) (Mazej et al, 2006). Data of content of selenium and its species in different plants are shown in Table 1.…”

Solvent Extraction, Spectrophotometric Determination of Selenium (Iv) at Microgram Level Using O-Methylphenyl Thiourea as a Sensitive Reagent: Analysis of Pharmaceuticals, Synthetic Mixtures and Real Samples

“…These methods include gas chromatography-electron capture detection (GC-ECD) [3] , atomic absorption spectroscopy (AAS) [4][5][6][7] , atomic fluorescence spectroscopy (AFS) [8] , inductively coupled plasma optical emission spectrometry (ICP-OES) [9] , hydride generation system and atomic fluorescence spectrometer (HG-AFS) [10] , hydride generation atomic fluorescence spectrometer (HG-AFS) [11] , stripping voltammetry [12] and integrated coupled plasma mass spectroscopy. [13] From the literature survey, it is clear that, different instrumental techniques are available for the determination of selenium (IV) in variety of samples.…”