Determination of senkirkine and senecionine in Tussilago farfara using microwave-assisted extraction and pressurized hot water extraction with liquid chromatography tandem mass spectrometry

Metabolic Fingerprinting of Tussilago farfara L. Using 1H‐NMR Spectroscopy and Multivariate Data Analysis

“…Recent pharmacological experiments revealed that the flower buds of T. farfara L. exhibit anti-oxidant effect, anti-microbial activity, a-glucosidase inhibitory effect, diacylglycerol acyltransferase inhibitory effect and inhibitory effect on nitric oxide synthesis in lipopolysaccharide (LPS)-activated macrophages (Gao et al, 2008;Park et al, 2008). Extensive chemical studies on the flower bud of T. farfara L. have been carried out, indicating the presence of several kinds of components, including essential oils (Liu et al, 2006), sesquiterpenes (Ryu et al, 1999;Yaoita et al, 1999Yaoita et al, , 2001, triterpenes (Santer and Stevenson, 1962;Yaoita and Kikuchi, 1998), flavonoids (Kim et al, 2006;Liu et al, 2007;Wu et al, 2010), phenylpropanoids (Liu et al, 2007;Gao et al, 2008;Wu et al, 2010), chromones (Wu et al, 2008) and pyrrolizidine alkaloids (Jiang et al, 2009). However, to our knowledge, no systematic chemical study on the leaves and fully open flowers has been carried out.…”

Evaluation of microwave‐assisted extraction for aristolochic acid from Aristolochiae Fructus by chromatographic analysis coupled with nephrotoxicity studies

“…It provides considerable reductions in extraction time and solvent consumption with improved extraction rate and to some extent selective extraction. Therefore, MAE has been widely used in the extraction of biologically active compounds from natural products (Yan et al , ; Xiao et al , , b; Du et al , ; Jiang et al , ).…”

The analysis of pyrrolizidine alkaloids in Jacobaea vulgaris; a comparison of extraction and detection methods

“…Gas chromatography (GC) with fl ame ionisation detection (FID) or nitrogen phosphorus detection (NPD) have been typically used as analytical methods (Hartmann and Toppel, 1987;Witte et al, 1992;Hartmann and Dierich, 1998;Kowalchuk et al, 2006). Over the years several other PA extraction methods (Pieters et al, 1989;Betteridge and Colegate, 2005;Joosten et al, 2009;Jiang et al, 2009) and techniques for analysing PAs have been applied such as spectrophotometry (Mattocks, 1967;De Boer, 1999), TLC, HPLC-UV, GC-MS (Hartmann and Toppel, 1987;Witte et al, 1992;Hartmann and Dierich, 1998) and NMR (Pieters et al, 1989, Leiss et al, 2009. Recently liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been introduced for measuring PAs in plant material (Wuilloud et al, 2004;Betteridge and Colegate, 2005;Zhang et al, 2008;Jiang et al, 2009;Joosten et al, 2009).…”