Determination of serotonin and its precursors in human plasma by capillary electrophoresis–electrospray ionization–time-of-flight mass spectrometry

Peterson, Zlatuše D.; Lee, Milton L.; Graves, Steven W.

doi:10.1016/j.jchromb.2004.07.022

Cited by 20 publications

(18 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Capillary electrophoresis or gas chromatography with electron capture (ECD), or nitrogen phosphorus detection (NPD) or mass spectrometric (MS) detection were used for the determination of serotonin in human plasma (Peterson, Lee & Graves, 2004).…”

Section: Introductionmentioning

confidence: 99%

New Spectrofluorimetric Method for Determining Serotonin: Application to Human Urine

Khonté¹,

Thiaré²,

Diop³

et al. 2015

IJC

View full text Add to dashboard Cite

In this present paper, we investigate the development of simple, rapid, accurate, reproducible and sensitive methods for the determination of serotonin (5-HT) in urine. For this purpose stationary fluorescence was used as method. With regard to the analysis of serotonin, the liquid-liquid extraction (LLE) solid phase extraction (SPE) and standard addition procedures were used. Several physicochemical factors affecting the sensitivity of the fluorescence intensity of serotonin were optimized, including the system of solvent (organic, micellar), the pH and the salts. The study of the analytical performances of the method led to very low limits of detection (LOD) varying between 0.1 and 3 ng/mL and to limits of quantification (LOQ) ranging between 0.4 and 10 ng/mL. This confirms the sensitivity of the method. Thus the low values of standard deviations (DRS) (between 0.3 and 6.6%) testify the good reproducibility of the measurements with satisfactory covering rate (89 to 111%). Accordingly, our results show that the spectrofluorimetric method is simple, fast and sensitive and can be applied to the routine analysis and does not require expensive equipment nor tiresome chemical pretreatments.Keywords: serotonin, effects of solvent, method of analysis of fluorescence direct, analysis of urine

show abstract

Section: Introductionmentioning

confidence: 99%

New Spectrofluorimetric Method for Determining Serotonin: Application to Human Urine

Khonté¹,

Thiaré²,

Diop³

et al. 2015

IJC

View full text Add to dashboard Cite

show abstract

“…Monaghan et al [17] developed a method for the analysis of serotonin in platelet depleted plasma for use as a biomarker in conditions such as functional gastrointestinal disorders. Protein precipitation was used for sample preparation followed by a cation exchange on-line clean-up step with analysis by LC-MS/ MS. Peterson et al [9] determined serotonin levels in serum using capillary electrophoresis-TOF-MS. The method removed both interferences and concentrated analytes from the serum using SPE with Oasis MXC (mixed mode cation exchange) columns.…”

Section: Resultsmentioning

confidence: 99%

“…It is involved in regulating blood pressure, smooth muscle contraction, pain sensation, it controls many brain functions and has been associated with sleep disruption and aggression. It has also been implicated in several pathological conditions, such as hypertension, migraine, depression, schizophrenia, anxiety, anorexia nervosa, dementia, and carcinoid syndrome [3][4][5][6][7][8][9]. Abnormal concentrations in plasma have been shown to reflect the serotonergic function in the central nervous system [4].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Application and Validation of HybridSPE-Precipitation Cartridge Technology for the Rapid Clean-up of Serum Matrices (from Phospholipids) for the Clinical Analysis of Serotonin, Dopamine and Melatonin

et al. 2012

View full text Add to dashboard Cite

Phospholipids have been shown to cause matrix effects particularly in liquid chromatography-mass spectrometry (LC-MS) analysis of small molecules. This results in suppression of the analyte signal. This study provides a versatile validated method for the analysis of serotonin in serum along with dopamine and melatonin using LC-MS/MS. It utilises HybridSPE-Precipitation cartridges for the clean-up of serum samples. This technology involves a simple protein precipitation step together with a fast and robust SPE method that is designed to remove phospholipids. Serotonin and dopamine are major neurotransmitters in the brain which affect various functions both in the brain and in the rest of the body. Melatonin plays an important role in the regulation of circadian sleep-wake cycle. Good linear calibrations were obtained for the multiplex assay of analytes in serum samples (0.021-3.268 lmol L -1 ; R 2 = 0.9983-0.9993). Acceptable intra-and inter-day repeatability was achieved for all analytes in serum. Excellent limits of detection (LOD) and limits of quantitation (LOQ) were achieved with LODs of 3.2-23.5 nmol L -1 and the LOQs of 15.4-70.5 nmol L -1 for these analytes in serum. The sample clean-up procedure that was developed provided efficient recovery and reproducibility while also decreasing preparation time and solvent use. A sample storage protocol was established, this was achieved by investigation of sample stability under different storage conditions. Evaluation of matrix effects was also carried out and the influence of ion suppression on analytical results reported. This clean-up protocol was then applied to the analysis of clinical serum samples.

show abstract

“…Admittedly, these detection modes provide better sensitivity than UV-vis but they require the derivatization step and high costs make them unaffordable for most laboratories. On the other hand, CE can be coupled to MS detectors which improves sensitivity [24], however, most of the CE methods for indoleamines separation require the use of wide ranges of pH and nonvolatile buffers, and the addition of micelle forming agents. Therefore, the use of the UV-vis for the detection of BAs is favored because these detectors are available in most laboratories and provides satisfactory quantification for some of the BAs [25].…”

Section: Introductionmentioning

confidence: 99%

Cyclodextrin-modified MEKC method for quantification of selected acidic metabolites of catecholamines in the presence of various biogenic amines. Application to diagnosis of neuroblastoma

Miękus

Kowalski

Olędzka

et al. 2015

Journal of Chromatography B

View full text Add to dashboard Cite

Determination of serotonin and its precursors in human plasma by capillary electrophoresis–electrospray ionization–time-of-flight mass spectrometry

Cited by 20 publications

References 48 publications

New Spectrofluorimetric Method for Determining Serotonin: Application to Human Urine

New Spectrofluorimetric Method for Determining Serotonin: Application to Human Urine

The Application and Validation of HybridSPE-Precipitation Cartridge Technology for the Rapid Clean-up of Serum Matrices (from Phospholipids) for the Clinical Analysis of Serotonin, Dopamine and Melatonin

Cyclodextrin-modified MEKC method for quantification of selected acidic metabolites of catecholamines in the presence of various biogenic amines. Application to diagnosis of neuroblastoma

Contact Info

Product

Resources

About