Determination of serotonin and its precursors in chocolate samples by capillary liquid chromatography with mass spectrometry detection

Guillén-Casla, Vanesa; Rosales-Conrado, Noelia; Pérez-Arribas, L.V.; Polo-Díez, L.M.

doi:10.1016/j.chroma.2011.11.037

Cited by 40 publications

(23 citation statements)

References 43 publications

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“…The main BAs found in cocoa beans and chocolate are 2-phenylethylamine, tyramine, serotonin and histamine, but also putrescine, dopamine and ethanolamine have been also found Granvogl et al 2006;Granvogl & Schieberle 2007;Mayer et al 2010;Guillén-Casla et al 2012;Oracz & Nebesny 2014). β-Phenylethylamine is an endogenous trace amine that occurs naturally in the brain of many mammalian species including humans (Bardocz 1995).…”

Section: Introductionmentioning

confidence: 98%

Determination of biogenic amine profiles in conventional and organic cocoa-based products

Restuccia

Spizzirri

Puoci

et al. 2015

Food Additives & Contaminants: Part A

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Cocoa contains many compounds such as biogenic amines (BAs), known to influence consumer health. Spermidine, spermidine, putrescine, histamine, tyramine, β-phenylethylamine, cadaverine and serotonine have been found in several cocoa-based products using HPLC with UV detection after derivatisation with dansyl-chloride. Once optimised in terms of linearity, percentage recovery, LOD, LOQ and repeatability, this method was applied to real samples. Total concentrations of BAs ranged from 5.7 to 79.0 µg g(-)(1) with wide variations depending on the type of sample. BAs present in all samples were in decreasing order: histamine (1.9-38.1 µg g(-)(1)) and tyramine (1.7-31.7 µg g(-)(1)), while putrescine (0.9-32.7 µg g(-)(1)), spermidine (1.0-9.7 µg g(-)(1)) and spermidine (0.6-9.3 µg g(-)(1)) were present in most of the samples. Cadaverine, serotonine and β-phenylethylamine were present in a few samples at much lower concentrations. Organic samples always contained much lower levels of BAs than their conventional counterparts and, generally speaking, the highest amounts of BAs were found in the most processed products.

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Section: Introductionmentioning

confidence: 98%

Determination of biogenic amine profiles in conventional and organic cocoa-based products

Restuccia

Spizzirri

Puoci

et al. 2015

Food Additives & Contaminants: Part A

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“…Therefore, quantitative analysis of 5‐HT in human urine and plasma is essential because of its coexistence in biological systems and regulating several physiological functions. Various sensing techniques have been examined for the determination of 5‐HT which were equipped with high performance liquid chromatography 1–3, mass spectroscopy 4, coulometry 5, capillary electrophoresis 6 and flow injection analysis 7, however these techniques require expensive instruments and are time consuming for sample preparation and derivatization processes which result in low recoveries and very high detection times compared to electroanalytical methods. Electrochemical techniques based on various methodologies have been developed to overcome these difficulties.…”

Section: Introductionmentioning

confidence: 99%

Nanobiocomposite Based Electrochemical Sensor for Sensitive Determination of Serotonin in Presence of Dopamine, Ascorbic Acid and Uric Acid In Vitro

2014

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A multiwalled carbon nanotube – chitosan (MWCNT‐Chit) composite modified glassy carbon electrode (GCE) was fabricated and applied for sensitive and selective determination of serotonin also known as 5‐hydroxytryptamine (5‐HT). Electroanalytical response of the fabricated electrode towards the detection of 5‐HT was studied by using cyclic voltammetry and electrochemical impedance spectroscopy. The results show that the MWCNT‐Chit/GCE has greatly enhanced the oxidation peak current and that the nanocomposite film enhanced the electron transfer to the analyte. Applying differential pulse voltammetry method, a linear calibration plot has been achieved over the concentration range 5×10−8–1.6×10−5 M and the low‐detection‐limit is 5×10−8 M. Selective determination of serotonin in the presence of dopamine, ascorbic acid and uric acid was established. The fabricated biosensor detects 5‐HT in‐vitro directly from artificial urine samples and from diluted serum samples.

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“…It plays numerous functions in the human body including the control of appetite, sleep, memory and learning, temperature regulation, mood, behavior, cardiovascular function, muscle contraction, endocrine regulation and depression. Guillén-Casla and coworkers developed a cLC–MS method for the analysis of serotonin (5-HT) and its precursors (5-HTP and TP) in chocolate samples [46]. The authors used acidic digestion for the extraction of target species in chocolate samples.…”

Section: Determination Of Neurochemicals By Lc-ms Related Techniquesmentioning

confidence: 99%

Recent Advances in Mass Spectrometry for the Identification of Neurochemicals and their Metabolites in Biofluids

Kailasa

2013

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Recently, mass spectrometric related techniques have been widely applied for the identification and quantification of neurochemicals and their metabolites in biofluids. This article presents an overview of mass spectrometric techniques applied in the detection of neurological substances and their metabolites from biological samples. In addition, the advances of chromatographic methods (LC, GC and CE) coupled with mass spectrometric techniques for analysis of neurochemicals in pharmaceutical and biological samples are also discussed.

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Determination of serotonin and its precursors in chocolate samples by capillary liquid chromatography with mass spectrometry detection

Cited by 40 publications

References 43 publications

Determination of biogenic amine profiles in conventional and organic cocoa-based products

Determination of biogenic amine profiles in conventional and organic cocoa-based products

Nanobiocomposite Based Electrochemical Sensor for Sensitive Determination of Serotonin in Presence of Dopamine, Ascorbic Acid and Uric Acid In Vitro

Recent Advances in Mass Spectrometry for the Identification of Neurochemicals and their Metabolites in Biofluids

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