Determination of serotonin, melatonin and metabolites in gastrointestinal tissue using high‐performance liquid chromatography with electrochemical detection

Chau, Rosanna M. W.; Patel, Bhavik Anil

doi:10.1002/bmc.1100

Cited by 39 publications

(35 citation statements)

References 31 publications

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“…This detection limit is significantly lower than that reported in other works for 5-HT using differential pulse voltammetry with bare carbon fiber (1.05 μM) and boron doped diamond (3.08 μM) microelectrodes 44. This is also lower than the detection limit obtained using high-liquid performance chromatography with electrochemical detection (241 nM) 29. The low detection limit and high sensitivity of our sensors allowed us to use this technology for determining 5-HT levels in the digestive system of intact embryos, where the concentrations are low.…”

Section: Resultscontrasting

confidence: 57%

Electrochemical Quantification of Serotonin in the Live Embryonic Zebrafish Intestine

et al. 2010

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We monitored real-time in vivo levels of serotonin release in the digestive system of intact zebrafish embryos during early development (5 dpf) using differential pulse voltammetry with implanted carbon fiber microelectrodes modified with carbon nanotubes dispersed in nafion. A detection limit of 1 nM, a linear range between 5 to 200 nM and a sensitivity of 83.65 nA·μM −1 were recorded. The microelectrodes were implanted at various locations in the intestine of zebrafish embryos. Serotonin levels of up to 29.9(±1.13) nM were measured in vivo in normal physiological conditions. Measurements were performed in intact live embryos without additional perturbation beyond electrode insertion. The sensor was able to quantify pharmacological alterations in serotonin release and provide the longitudinal distribution of this neurotransmitter along the intestine with high spatial resolution. In the presence of fluvoxamine, a selective serotonin reuptake inhibitor (SSRI), concentrations of 54.1(±1.05) nM were recorded while in the presence of p-chloro-phenylalanine (PCPA), a tryptophan hydroxylase inhibitor, the serotonin levels decreased to 7.2(±0.45) nM. The variation of serotonin levels was correlated with immunohistochemical analysis. We have demonstrated the first use of electrochemical microsensors for in vivo monitoring of intestinal serotonin levels in intact zebrafish embryos.

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Section: Resultscontrasting

confidence: 57%

Electrochemical Quantification of Serotonin in the Live Embryonic Zebrafish Intestine

et al. 2010

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“…For the evaluation of electroactive surface area of the working electrode among different common benchmark redox systems, K 4 [Fe(CN) 6 ] was selected for its surface sensitive electrochemical response, especially for carbonaceous materials. 3-redox pair present in solution in anodic and cathodic scans.…”

Section: Voltammetric Characterization Of Graphene-based Sensormentioning

confidence: 99%

“…3,4 Numerous analytical methods were reported for detection and quantification of melatonin, such as ultraviolet-visible (UV-vis) spectrophotometry, chemilumenescence, fluorometry, thin-layer chromatography, gas chromatography-mass spectrometry, and high-performance liquid chromatography. [5][6][7][8][9] Furthermore, melatonin is an antioxidant compound that can be analyzed with electrochemical methods. 10 Voltammetric and amperometric methods were applied for the determination of melatonin using carbon paste electrodes, carbon disk electrode, carbon fiber microdisk electrode, etc.…”

Section: Introductionmentioning

confidence: 99%

Voltammetric determination of melatonin using a graphene-based sensor in pharmaceutical products

Apetrei

2016

IJN

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Melatonin can be sensitively detected in pharmaceuticals by cyclic voltammetry and fixed-potential amperometry using a graphene-based sensor. The sensor characterization of cyclic voltammetry constantly provides high values of electrode active area and heterogeneous rate constant. In optimal conditions, the sensor was applied for the determination of melatonin in different pharmaceutical samples. The sensitivity to melatonin was 0.0371 A M −1 , and the limit of detection was 0.87×10 −6 M. The data obtained by using the graphene-based sensor for the detection of melatonin in pharmaceutical products were in good agreement with the data provided by the producer. Since no interferences from the excipients were found, using a separation technique was not necessary. Additionally, the low price, ease of handling, small amount of sample, short time per analysis, and possibility of automation are the important advantages that recommend this methodology for quality control of pharmaceuticals.

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“…This is expected as shown in Scheme 1 only 5-HT is released from the EC cell and thus can be monitored. At present the majority of assays studying the efficacy of the transmission process rely on monitoring homogenised tissue samples, which can only monitor intracellular components [18,28,30]. Therefore assumptions about the transmission mechanism are being made based on what can be monitored and thus for example intracellular 5-HT is often used as a marker of release, even if only a fraction of this is actually released [33,34].…”

Section: Resultsmentioning

confidence: 99%

“…However, in contrast, high performance liquid chromatography (HPLC) [18,29,30] is capable of measuring all parameters noted in Scheme 1. Within the field of gastroenterology, HPLC has been predominantly used to measure levels of analytes in tissue homogenates [18,28,30] and hence only intracellular neurochemicals are monitored, meaning the release of 5-HT from EC cells is entirely missed. On the other hand, there are methods such as microdialysis [31,32] that only measure extracellular levels of 5-HT and so intracellular neurochemicals are not taken into account.…”

Section: Introductionmentioning

confidence: 99%

Chromatographic analysis of age-related changes in mucosal serotonin transmission in the murine distal ileum

Parmar

Fidalgo

Yeoman

et al. 2012

Chemistry Central Journal

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BackgroundIn the upper bowel, alterations in motility and absorption of key nutrients have been observed as part of the normal ageing process. Serotonin (5-HT) is a key signalling molecule in the gastrointestinal tract and is known to influence motility, however little is known of how the ageing process alters 5-HT signalling processes in the bowel.ResultsAn isocratic chromatographic method was able to detect all 5-HT precursors and metabolites. Using extracellular and intracellular sampling approaches, we were able to monitor all key parameters associated with the transmission process. There was no alteration in the levels of tryptophan and 5-HTP between 3 and 18 month old animals. There was a significant increase in the ratio of 5-HT:5-HTP and an increase in intracellular 5-HT between 3 and 18 month old animals suggesting an increase in 5-HT synthesis. There was also a significant increase in extracellular 5-HT with age, suggesting increased 5-HT release. There was an age-related decrease in the ratio of intracellular 5-HIAA:extracellular 5-HT, whilst the amount of 5-HIAA did not change with age. In the presence of an increase in extracellular 5-HT, the lack of an age-related change in 5-HIAA is suggestive of a decrease in re-uptake via the serotonin transporter (SERT).ConclusionsWe have used intracellular and extracellular sampling to provide more insight into alterations in the neurotransmission process of 5-HT during normal ageing. We observed elevated 5-HT synthesis and release and a possible decrease in the activity of SERT. Taken together these changes lead to increased 5-HT availability and may alter motility function and could lead to the changes in adsorption observed in the elderly.

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Determination of serotonin, melatonin and metabolites in gastrointestinal tissue using high‐performance liquid chromatography with electrochemical detection

Cited by 39 publications

References 31 publications

Electrochemical Quantification of Serotonin in the Live Embryonic Zebrafish Intestine

Electrochemical Quantification of Serotonin in the Live Embryonic Zebrafish Intestine

Voltammetric determination of melatonin using a graphene-based sensor in pharmaceutical products

Chromatographic analysis of age-related changes in mucosal serotonin transmission in the murine distal ileum

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