Determination of Serum Glycoproteins

Winzler, Richard J.

doi:10.1002/9780470110188.ch10

Cited by 873 publications

(92 citation statements)

References 142 publications

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“…Isolated BBM were assessed for their ability to transport glucose by uptake measurements carried out using the rapid filtration technique, at room temperature as described. 27 Hexoses, fucose, 28 sialic acid, 29 and hexosamine content 30 of surfactant and BBM were determined as described and expressed as nanomoles per milligram protein.…”

Section: Methodsmentioning

confidence: 99%

Intestinal mucosal alterations in rats with carbon tetrachloride‐induced cirrhosis

et al. 2006

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Spontaneous bacterial peritonitis is a major cause of mortality after liver cirrhosis. Altered permeability of the mucosa and deficiencies in host immune defenses through bacterial translocation from the intestine due to intestinal bacterial overgrowth have been implicated in the development of this complication. Molecular mechanisms underlying the process are not well known. In order to understand mechanisms involved in translocation of bacteria, this study explored the role of oxidative stress in mediating changes in intestinal mucosal glycosylation and luminal bacterial content during cirrhosis. CCl 4 -induced cirrhosis in rats led to prolonged oxidative stress in the intestine, accompanied by increased sugar content of both intestinal brush border and surfactant layers. This was accompanied by changes in bacterial flora in the gut, which showed increased hydrophobicity and adherence to the mucosa. Inhibition of xanthine oxidase using sodium tungstate or antioxidant supplementation using vitamin E reversed the oxidative stress, changes in brush border membrane sugar content, and bacterial adherence. In conclusion, oxidative stress in the intestine during cirrhosis alters mucosal glycosylation, accompanied by an increased hydrophobicity of luminal bacteria, enabling increased bacterial adherence onto epithelial cells. This might facilitate translocation across the mucosa, resulting in complications such as spontaneous bacterial peritonitis. (HEPATOLOGY 2006;43:837-846.)

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Section: Methodsmentioning

confidence: 99%

Intestinal mucosal alterations in rats with carbon tetrachloride‐induced cirrhosis

et al. 2006

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“…The method used for the determination of acidsoluble glycoprotein (ASG) levels was based on the method of Winzler (1955) as modified by Nahagata et al (1989) and Eckersall et al (1996). Commercially available purified bovine a-1-acid glycoprotein (AGP, Sigma Chemical Co., St. Louis, Missouri, USA) was used as a standard because AGP is the main constituent in human ASG.…”

Section: Biochemical Analysesmentioning

confidence: 99%

Acute Phase Protein Response in the Capybara (Hydrochoerus Hydrochaeris)

Bernal

Feser

Martı́nez-Subiela

et al. 2011

Journal of Wildlife Diseases

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ABSTRACT:We evaluated the acute phase protein response in capybaras (Hydrochoerus hydrochaeris). Three animal groups were used: 1) healthy animals (n530), 2) a group in which experimental inflammation with turpentine was induced (n56), and 3) a group affected with sarcoptic scabies (n514) in which 10 animals were treated with ivermectin. Haptoglobin (Hp), acid-soluble glycoprotein (ASG) and albumin were analyzed in all animals. In those treated with turpentine, Hp reached its maximum value at 2 wk with a 2.7-fold increase, whereas ASG increased 1.75-fold and albumin decreased 0.87-fold 1 wk after the induction of inflammation. Capybaras affected with sarcoptic scabies presented increases in Hp and ASG of 4.98-and 3.18-fold, respectively, and a 0.87-fold decrease in albumin, compared with healthy animals. Haptoglobin and ASG can be considered as moderate, positive acute phase proteins in capybaras because they showed less than 10-fold increases after an inflammatory process and reached their peak concentrations 1 wk after the induction of inflammation. Conversely, albumin can be considered a negative acute phase protein in capybaras because it showed a reduction in concentration after inflammatory stimulus.

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“…All the serum samples were stored at -70~ until analysis. Biochemical anaIysis was performed on serum samples for estimation of protein bound hexose, protein bound hexosamine, protein bound sialic acid, protein bound fucose and protein bound carbohydrate (16,18). All the reagents were of analytical reagent (AR) grade.…”

Section: Methodsmentioning

confidence: 99%

“…A person diagnosed with major depressive episode must also experience at least four symptoms from a list that includes changes in appetite and weight, changes in sleep and activity, lack of energy, feeling of guilt, problems in thinking and making decision and recurring thoughts of death or suicide. Since the glycoproteins vary in their relative content of serum of four monosaccharides, the net change in the total level would also vary, depending upon the monosaccharides used for determination (15,16). In psychiatric disorders such as schizophrenia, elevated levels of serum glycoprotein has been reported (17).…”

Section: Introductionmentioning

confidence: 99%

Changes in levels of serum glycoproteins in major depressive disorders

Nandave

Ojha

Kaur

2005

Indian J Clin Biochem

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The present study deals with estimation of levels of fractions of serum glycoproteins, protein bound hexose (PBH), protein bound hexosamine (PBHex), protein bound fucose (PBF), protein bound sialic acid (PBS) and protein bound carbohydrate (PBC) in thirty patients of Major Depressive Disorders (MDD) in comparison with thirty normal subjects. In patients of MDD, the level of PBH, PBHex, PBF, PBS and PBC were significantly higher as compared to the normal subjects (p<0.05). In patients of MDD, after one-month treatment with fluoxetine, the levels of PBH, PBHex, PBF, PBS and PBC were significantly decreased as compared to the levels of these fractions in same patients of MDD before beginning of the treatment (p<0.05). Based on findings of the present study, it can be concluded that changes in the level of serum glycoproteins level before and after treatment with fluoxetine can be correlated with clinical status of MDD.

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Determination of Serum Glycoproteins

Cited by 873 publications

References 142 publications

Intestinal mucosal alterations in rats with carbon tetrachloride‐induced cirrhosis

Intestinal mucosal alterations in rats with carbon tetrachloride‐induced cirrhosis

Acute Phase Protein Response in the Capybara (Hydrochoerus Hydrochaeris)

Changes in levels of serum glycoproteins in major depressive disorders

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