Determination of seven arsenic species in seafood by ion exchange chromatography coupled to inductively coupled plasma-mass spectrometry following microwave assisted extraction: Method validation and occurrence data

Leufroy, Axelle; Noël, Laurent; Dufailly, Vincent; Beauchemin, Diane; Guérin, Thierry

doi:10.1016/j.talanta.2010.10.050

Cited by 104 publications

(60 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The challenge in chromatographic separation of arsenic species lies within their chemical nature, including different charges and pKa values, molecular sizes and functional groups. Several liquid chromatography approaches have been applied for the separation of arsenic compounds in combination with inductively coupled plasma mass spectrometry (ICP-MS) as an arsenic-specific detector, with anion exchange chromatography as the predominant technique of choice [18][19][20].…”

Section: Introductionmentioning

confidence: 99%

“…The recent increased emphasis on dietary iAs exposure, partly encouraged by the recent EFSA [3] and WHO [11] evaluations, have initiated several studies on specific determination of iAs levels in foodstuffs [7,19,21], in all of which iAs is determined by HPLC-ICP-MS. However, many laboratories involved in feed and food control do not have the ICP-MS technique available, mainly due to the relatively high cost of investment for this technology compared with other elemental detection techniques like, e.g.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Development and validation of an SPE HG-AAS method for determination of inorganic arsenic in samples of marine origin

et al. 2012

View full text Add to dashboard Cite

The present paper describes a novel method for the quantitative determination of inorganic arsenic (iAs) in food and feed of marine origin. The samples were subjected to microwave-assisted extraction using diluted hydrochloric acid and hydrogen peroxide, which solubilised the analytes and oxidised arsenite (As(III)) to arsenate (As(V)). Subsequently, a pH buffering of the sample extract at pH 6 enabled selective elution of As(V) from a strong anion exchange solid-phase extraction (SPE) cartridge. Hydride generation atomic absorption spectrometry (HG-AAS) was applied to quantify the concentration of iAs (sum of As(III) and As(V)) as the total arsenic (As) in the SPE eluate. The results of the in-house validation showed that mean recoveries of 101-104% were achieved for samples spiked with iAs at 0.5, 1.0 and 1.5 mg·kg(-1), respectively. The limit of detection was 0.08 mg kg(-1), and the repeatability (RSD(r)) and intra-laboratory reproducibility (RSD(IR)) were less than 8% and 13%, respectively, for samples containing 0.2 to 1.5 mg kg(-1) iAs. The trueness of the SPE HG-AAS method was verified by confirming results obtained by parallel analysis using high-performance liquid chromatography coupled to inductively coupled plasma mass spectrometry. It was demonstrated that the two sets of results were not significantly different (P < 0.05). The SPE HG-AAS method was applied to 20 marine food and feed samples, and concentrations of up to 0.14 mg kg(-1) of iAs were detected.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development and validation of an SPE HG-AAS method for determination of inorganic arsenic in samples of marine origin

et al. 2012

View full text Add to dashboard Cite

show abstract

“…The most commonly employed method is to evaluate LOQ as 3 times the LOD [1]. In some papers, LOQ was calculated as 10 times the LOD, 6 or 10 times the standard deviation of the blank sample [24][25][26]. Other authors have estimated LOQ using the standard deviation/slope method described above [27,28].…”

Section: Lod and Loqmentioning

confidence: 99%

Speciation analysis of chromium in drinking water samples by ion-pair reversed-phase HPLC–ICP-MS: validation of the analytical method and evaluation of the uncertainty budget

et al. 2013

View full text Add to dashboard Cite

The approach presented in this article refers to the modification of a method for the detection and quantitative determination of chromium species in water by high-performance liquid chromatography inductively coupled plasma mass spectrometry. The main aim of this work was to establish a detailed validation of the analytical procedure and an estimation of the budget of measurement uncertainty which was helpful in recognizing the critical points of the presented method. As a result of the method validation experiment, the obtained limit of quantification, repeatability and intermediate precision were satisfied for the quantification Cr(III) and Cr(VI) in water matrices. The trueness of the method was verified via an estimation of the recovery of the spiked real samples. The recovery rate of both determined analytes was found to be between 93 and 115 %. Considering that the validation of the method and the evaluation of measurement uncertainty are crucial for quantitative analysis, the above-mentioned assessment of the uncertainty budget was performed in two different ways: a modelling approach and a single-laboratory validation approach. The measurement uncertainties of the results were found to be 4.4 and 7.8 % for Cr(III), 4.2 and 7.9 % for Cr(VI) using the classical concept and method validation data, respectively. This paper is the first publication to presenting all the steps needed to evaluate the measurement uncertainty for the speciation analysis of chromium species. In summary, the obtained results demonstrate that the method can be applied effectively for its intended use.

show abstract

“…But arsenobetaine (AsB) and arsenocholine (AsC) of organic arsenic species have been known to be nontoxic species. [8][9][10][11][12][13][14][15][16][17][18][19][20][21][22] In general, sea water includes 3 μg/kg of arsenic, and the arsenic can be accumulated and transformed to the different chemical forms in a marine life. 23 Arsenics are abundant in fishes, crustacean (lobster, shrimp, and crab), clam, and seaweed, and the chemical forms of arsenic are organic species that be easily excreted from the human body.…”

Section: Introductionmentioning

confidence: 99%

A Study on Arsenic Speciation in Korean Oyster Samples using Ion Chromatography Inductively Coupled Plasma Mass Spectrometry

Nam

Cui

et al. 2015

Bulletin Korean Chem Soc

View full text Add to dashboard Cite

The qualitative and quantitative analysis of the different arsenic species in Korean oyster samples was performed to provide the vital information of food safety of the marine sample in terms of the arsenic species. Korean marine samples, oysters, were obtained from nine sites in the western and southern areas of South Korea. Inductively coupled plasma mass spectrometry (ICP-MS) was used to measure the total arsenic. The different arsenic species in the oyster were separated by ion chromatography (IC), and then detected by ICP-MS. Extraction of arsenic species was performed with the mixed solvent of the methanol:water (3:1). The extraction efficiency of arsenic species (the ratio of total amount of the different arsenic species to the total arsenic in the oyster) for nine oysters was approximately 73-81%. The major arsenic species in the oyster was arsenobetaine, and its concentration in the samples was in the range of 7-16 mg/kg that was more than 90% of the total organic arsenic in the oyster. As(III) was not generally detected while the trace As(V) was detected in most oysters.Keywords: Arsenic speciation, Korean marine sample, Oyster, Ion chromatography, Inductively coupled plasma-mass spectrometry IntroductionArsenic has been known as a carcinogenic element that might be accumulated and biologically toxic in human body. [1][2][3][4][5] The element exists in various chemical forms, including trivalent and pentavalent states, inorganic and organic compounds, and about 30 different chemical species has been reported ( Table 1). In general, arsenic species are classified as inorganic arsenic and organic arsenic species. Inorganic arsenic species were formed with oxygen, sodium, chlorine, and sulfur. Organic arsenic species were formed with carbon, hydrogen, and oxygen existed in the animal and plant. 4 Even though the toxicological mechanism was not revealed, the inorganic arsenic species have been reported to be more toxic than organic arsenic species. 6,7 In particular, arsenite (As(III)) is more toxic than arsenate (As(V)). As a small amount of inorganic arsenic species have been known to be fatal to human being, it had been used as an assassination chemical and deadly drug in the middle ages. But arsenobetaine (AsB) and arsenocholine (AsC) of organic arsenic species have been known to be nontoxic species. [8][9][10][11][12][13][14][15][16][17][18][19][20][21][22] In general, sea water includes 3 μg/kg of arsenic, and the arsenic can be accumulated and transformed to the different chemical forms in a marine life. 23 Arsenics are abundant in fishes, crustacean (lobster, shrimp, and crab), clam, and seaweed, and the chemical forms of arsenic are organic species that be easily excreted from the human body. In addition, inorganic arsenic could not be formed in a metabolism of organic arsenic in a mammal due to the strong bond of arsenic and carbon. Methylarsonic acid (MMA) and dimethylarsinic acid (DMA) could be restrictively metabolized, and excreted in a urine without changes of the chemical forms. Accordingly...

show abstract

Determination of seven arsenic species in seafood by ion exchange chromatography coupled to inductively coupled plasma-mass spectrometry following microwave assisted extraction: Method validation and occurrence data

Cited by 104 publications

References 51 publications

Development and validation of an SPE HG-AAS method for determination of inorganic arsenic in samples of marine origin

Development and validation of an SPE HG-AAS method for determination of inorganic arsenic in samples of marine origin

Speciation analysis of chromium in drinking water samples by ion-pair reversed-phase HPLC–ICP-MS: validation of the analytical method and evaluation of the uncertainty budget

A Study on Arsenic Speciation in Korean Oyster Samples using Ion Chromatography Inductively Coupled Plasma Mass Spectrometry

Contact Info

Product

Resources

About