Determination of silicon in serum and tissue by electrothermal atomic absorption spectrometry

Bioactive calcium silicate extracts regulate the morphology and stemness of human embryonic stem cells at the initial stage

“…As far as we know, the naturally occurring exposure concentration of silicon ions keep changed dynamically in different tissues or organs. A body of evidences indicates that serum Si concentration is ~0.1-0.2 μg/ml for most healthy adults 9,[46][47][48][49] , is peaked at ~0.8-1.2 μg/ml after intake of silicon-containing food 9, 46, 47 , and even yields 15-fold higher for hemodialysis patients up to ~3.3 μg/ml (their basal level is 0.9 μg/ml) when they take in silicon food 50 . In the current work, the plain medium used has similar silicon concentration in human physiological serum.…”

Silicon

“…Deuterium arc background correction Holden 1992, Wrobel 1993, Perez-Parajon 1994 ET-AAS Zeeman background correction -transverse configuration Gitelman 1990, Huang 1995, Kobayshi 1995, Saldanha 1997, Leung 1997 Van Dyck 1999 ICP-AES Wang 1989, Jackson 1998 DCP- AES Roberts 1990, Belliveau 1991, Bercowy 1994, Fahal 1993, Bellia et al 1994 Double focusing magnetic sector Riondato et al 1997 La(NO 3 ) 3 -CaCl 2 -NH 4 H 2 PO 4 -NA 4 EDTA· 4 H 2 O-HNO 3 modifier, metal carbide coating of graphite tubes or platforms was no longer required by Huang (1995), thereby allowing direct analysis of 20-fold diluted serum and 100-fold diluted urine samples against aqueous standards after atomization from the wall of pyrolytically coated graphite tubes. In our hands this method also offers the best analytical performance for analysis of serum and urine samples with low silicon levels, provided that the analyses are performed against matrix-matched calibration curves.…”

“…At the higher concentration range (e.g., serum of dialysis patients) an acceptable analytical performance could also be obtained after simple dilution in Triton X-100 (D'Haese et al , 2001. Kobayashi et al (1995) used pyrolytically coated graphite tubes in combination with a NiCl 2 modifier, whereas Leung and Edmond (1997) used a La 2 O 3 -CaCl 2 -NH 4 H 2 PO 4 -EDTAethanol-Triton X-100 mixture. Before injecting serum, water or urine samples diluted in Triton X-100 into the graphite furnace, these authors injected a Pd -Mg(NO 3 ) 2 mixture for matrix modification (Van Dyck 1999).…”

Silicon determination in milk by electrothermal atomic absorption spectrometry using palladium as chemical modifier

“…A variety of methods has been used for silicon determination including chemical methods [15], flame [16], and inductively coupled plasma atomic emission spectrometry [17,18,19], electrothermal atomic absorption spectrometry (ETAAS) [20,21,22,23] and neutral activation analysis [24].…”

“…As chemical modifier mixed Pd-Mg(NO 3 ) 2 was used by Kristein [26] for silicon determination in food supplements, lanthanum was used by Lueng [27] for silicon determination in serum and tissue and Zhuoer [28] proposed use of a mixture of La(NO 3 ) 3 , CaCl 2 , NH 4 H 2 PO 4 and Na 4 EDTA for soft tissue digests, whereas La(NO 3 ) 3 and tartaric acid disodium salt were used for analysis of bone digest.…”