Determination of Six Pterins in Urine By Lc–MS/MS

Allegri, Gabriella; Netto, Heleno José Bezerra Costa; Gomes, Luiz Nelson Lopes Ferreira; Oliveira, Maria Lúcia Costa de; Scalco, Fernanda B.; Neto, Francisco Radler de Aquino

doi:10.4155/bio.12.131

Cited by 19 publications

(15 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Specifically, Lugol's solution, comprising a 4%:2% (w/v) mixture of potassium iodide-iodine, is the most commonly used oxidative pretreatment for pteridine analysis and was evaluated herein in both alkaline and acidic environments per previously developed protocol [27,35,37]. Permanganate and acidic manganese dioxide oxidative efficiency were also investigated using earlier developed methods [28,44]. A summarized comparison of the effects of these pretreatments on pteridine standards prepared in synthetic urine has been provided in Table 3.…”

Section: Comparative Study Of Oxidative Pretreatmentsmentioning

confidence: 99%

“…More recent efforts have seen an attempt to develop robust analytical methodologies using advanced instrumental platforms and oxidative pretreatments to enable sensitive detection of biologically significant pteridine derivatives. To this end, a multitude of competent analytical techniques have emerged over the past several years using an array of sensitive instrumental platforms including capillary electrophoresislaser-induced fluorescence (CE-LIF) [35], high-performance liquid chromatographyfluorescence detection (HPLC-FD) [28,[36][37][38][39][40], high-performance liquid chromatographymass spectrometry (HPLC-MS) [41], high-performance liquid chromatographytandem mass spectrometry (HPLC-MS/MS) [29,[42][43][44], hydrophilic interaction chromatographytandem mass spectrometry (HILIC-MS/MS) [45], and synchronous fluorescence spectroscopy [46,47], which have been summarized in Table 1.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Development of a high-performance liquid chromatography – Tandem mass spectrometry urinary pterinomics workflow

Burton

Shi

2016

Analytica Chimica Acta

View full text Add to dashboard Cite

Pteridines have evoked considerable interest from the scientific community owing to their prominent roles in human health and disease. The availability of analytical methodologies suitable for comprehensive pteridine profiling, termed here as "pterinomics", has been limited by inconsistent sample preparation and the exclusion of lesser studied pteridine derivatives. In response, the present study describes a new pterinomics workflow using a high-performance liquid chromatography - tandem mass spectrometry (HPLC-MS/MS) methodology for the simultaneous analysis of 15 pteridine derivatives including four structural isomers, marking the largest quantitative pteridine panel that has been studied to-date. The validated method possessed excellent sensitivity with method detection limits (0.025 μg L(-1) to 0.5 μg L(-1)) that were comparable or superior to existing techniques. Spiked recovery studies demonstrated the technique was both accurate (88-112%) and precise (RSD: 0-6%). A comparative study of commonly used oxidative pretreatments, including triiodide, permanganate, and manganese dioxide, revealed that the oxidative mechanisms were inefficient, complex, and concentration dependent. Finally, 50 clinical urine specimens were examined with the new technique wherein 10 pteridine derivatives were quantified and population ranges have been given. This technique can be used to examine pteridine molecular epidemiology and biochemistry to support related research applications, and may further be readily extended to include additional pteridine derivatives and biological matrices for specific applications.

show abstract

Section: Comparative Study Of Oxidative Pretreatmentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of a high-performance liquid chromatography – Tandem mass spectrometry urinary pterinomics workflow

Burton

Shi

2016

Analytica Chimica Acta

View full text Add to dashboard Cite

show abstract

“…C18 has been popular, but we have found isocratic analysis on strong cation exchange (SCX) columns at pH 2.5 that were excellent for urine analysis [ 9 , 35 ]. However, for plasma analysis, we have found the amino column-based separation similarly reliable and efficient [ 55 ]. The amino column method was original designed for LC-MS, but we have found fluorescence detection to be more than adequate for most clinical needs.…”

Section: Measurement Of Neopterin and Total Neopterinmentioning

confidence: 99%

“…The two-step process to measure 7,8-dihydroneopterin can be avoided using HPLC coupled with mass spectrometry (LC-MS or LC-tandem mass spectrometry (LC-MS/MS)) [ 55 , 59 ]. The sensitivity of mass spectrometry has urinary neopterin and biopterin detection limits of 0.082 and 0.76 nM, respectively, which are much lower than fluorescence detection.…”

Section: Measurement Of Neopterin and Total Neopterinmentioning

confidence: 99%

Neopterin, Inflammation, and Oxidative Stress: What Could We Be Missing?

Gieseg

Lindsay

2018

Antioxidants

View full text Add to dashboard Cite

Neopterin has been extensively used as a clinical marker of immune activation during inflammation in a wide range of conditions and stresses. However, the analysis of neopterin alone neglects the cellular reactions that generate it in response to interferon-γ. Neopterin is the oxidation product of 7,8-dihydroneopterin, which is a potent antioxidant generated by interferon-γ-activated macrophages. 7,8-Dihydroneopterin can protect macrophage cells from a range of oxidants through a scavenging reaction that generates either neopterin or dihydroxanthopterin, depending on the oxidant. Therefore, plasma and urinary neopterin levels are dependent on both macrophage activation to generate 7,8-dihydroneopterin and subsequent oxidation to neopterin. This relationship is clearly shown in studies of exercise and impact-induced injury during intense contact sport. Here, we argue that neopterin and total neopterin, which is the combined value of 7,8-dihydroneopterin and neopterin, could provide a more comprehensive analysis of clinical inflammation than neopterin alone.

show abstract

“…SCX-HPLC has proven to be very useful for urinary analysis [6], with plasma samples there can be problems with fully resolving the compounds of interest, especially at the low concentrations observed. Recently, an amine column method has been developed for the determination of multiple pteridines in urine using tandem mass spectrometry [20]. While MS/MS is the ideal analytical tool for compound determination due to low detection limits, it adds considerable cost and complexity compared to fluorescence detection.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous analysis of neopterin, kynurenine and tryptophan by amine-HPLC shows minor oxidative stress from short-term exhaustion exercise

Baxter-Parker

Chu

Petocz³

et al. 2019

Pteridines

View full text Add to dashboard Cite

Introduction: Neopterin, kynurenine and tryptophan can be used to measure activation of monocytes and macrophages during immunological events such as exercise inducing inflammation. Endurance exercise and high-impact sports have shown significant increases in these biomarkers. Measurement is typically conducted by high-performance liquid chromatography (HPLC) using C18 or SCX columns. However, kynurenine and tryptophan are not measured simultaneously to neopterin using these separation systems. Here we have used an amine column for separation and simultaneous determination of neopterin, kynurenine and tryptophan. Methods: Optimization and validation for the amine-HPLC method was conducted using plasma from 43 participants subjected to a short maximal exercise bicycling regime or rest period. The order of exercise and rest was randomized and separated by a 3-5 week washout period. Results: Using an amine column developed with ammonium acetate formic acid (33%) and acetonitrile (72%) provided optimal separation and run time for analysis. Neopterin increased significantly post-exercise and subsided to baseline by 30 minutes. Total neopterin remained elevated until 60 minutes following exercise. Conclusion: Amine-HPLC can be used for simultaneous determination of kynurenine, tryptophan and neopterin in plasma. Short intense exercise causes a significant increase in plasma neopterin suggesting a prolonged activation of monocytes and macrophages.

show abstract

Determination of Six Pterins in Urine By Lc–MS/MS

Cited by 19 publications

References 24 publications

Development of a high-performance liquid chromatography – Tandem mass spectrometry urinary pterinomics workflow

Development of a high-performance liquid chromatography – Tandem mass spectrometry urinary pterinomics workflow

Neopterin, Inflammation, and Oxidative Stress: What Could We Be Missing?

Simultaneous analysis of neopterin, kynurenine and tryptophan by amine-HPLC shows minor oxidative stress from short-term exhaustion exercise

Contact Info

Product

Resources

About