Determination of Sodium Saccharin in Chewing Gum by High Pressure Liquid Chromatography

Abstract

A method is presented for the precise and accurate determination of sodium saccharin in chewing gum utilizing high pressure liquid chromatography. The sodium saccharin is extracted into a biphasic solvent system and quantitated by the internal standard method. The procedure presented exhibits a percent recovery of 101.3% with a standard deviation of 2.6%. Results of the analysis of five different flavors of two commercial brands of sugarless chewing gum indicate a range of 79% to 116% of label claim. No evidence of chemical degradation of the sodium saccharin was found.

Use of high-pressure liquid chromatography for analysis of sweet potato phenolics

“…The internal standard method of quantitation was used to compensate for errors in the analytical procedure (Eng et al, 1977). To standardize the procedure, a series of solutions containing 20-125 Mg/mL of chlorogenic acid (CA) and 100 Mg/mL of coumarin (internal standard) were injected, and the peak areas were measured with a planimeter.…”