Determination of Soluble Salts in Interstitial Water of Fluvial Sediments by Ie-HPLC

The current experiment is designed to evaluate the effect of different aquafeeds (farm-made versus commercial) on growth, body composition, oxidative capacity, and fatty acid profile in the semi-intensive composite culture system. For this, 1,100 fingerlings/acre having initial body weight and length, Labeo rohita (61.34 g, 171 mm), Catla catla (71.45 g, 181 mm), and Cyprinus carpio (30.80 g, 91 mm) were randomly distributed to 16 ponds and randomly fed on eight different diets (n = 2 pond/diet) in a completely randomized research design. Aquafeed were farm-based diets (D1–D2) and commercial aquafeed (D3–D8). The farm-made diets contained various crude protein levels of maize gluten (24.9%) and rice polish (7.3%), whereas commercial diets were procured from commercial feed plants (AMG, Supreme, Aqua, Star Floating, Hi-Pro, and Punjab feed). The growth performance of carps (L. rohita and C. catla) was significantly improved (p<0.05) by feeding D3 as compared to other diets. Similarly, white blood cell concentration was greater (p<0.05) in all species fed by D3 than in those fed on D7, D8, D5, D6, D1, and D2 fed groups, respectively. Alanine transaminase, aspartate transaminase, and alanine phosphatase activities were significantly lower (p<0.05) in the D3-fed L. rohita, C. catla, and C. carpio compared with those fed on the rest of the treatments. The activities of glutathione peroxidase and superoxide dismutase were also higher (p<0.05) for the D3 fed L. rohita, C. catla, and C. carpio than those fed on the rest diets. The groups fed on D3 and D4 had greater (p<0.05) concentrations of myristic (14), palmitic acid (16), and stearic (18) acids than those fed on the rest of the commercial diets. However, meat chemical composition was similar (p>0.05) across the treatments. These results also prove that the increase in the dietary protein level and lipid content can improve the fish’s body’s crude protein and fat levels. Feeding D3 improved the production performance, oxidative status, and fatty acid profile in composite major carps culture systems. Thus, based on growth, survival, and body composition, it is concluded that D3 and D4 may be recommended for a commercial culture of major carps. Dietary treatments had no significant impact (p>0.05) on water’s physical–chemical properties. Calcium content and alkalinity varied (p<0.05), with D5 showing the lowest calcium and the highest alkalinity.

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Biomarker selection depends on gene function and organ: the case of the cytochrome P450 family genes in freshwater fish exposed to chronic pollution

Cortés-Miranda,

Rojas-Hernández,

Muñoz

et al. 2024

PeerJ

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Pollution and its effects have been of major concern in recent decades. Many strategies and markers have been developed to assess their effects on biota. Cytochrome P450 (CYP) genes have received significant attention in this context because of their relationship with detoxification and activation of exogenous compounds. While their expression has been identified as a pollution exposure biomarker, in most cases, it has been tested only after acute exposures and for CYP genes associated with exogenous compounds. To elucidate CYP gene expression patterns under chronic pollution exposure, we have used the silverside Basilichthys microlepidotus as a model, which inhabits the Maipo River Basin, a freshwater system with different pollution levels. We performed next-generation RNA sequencing of liver and gill tissues from polluted and non-polluted populations. We found most CYP genes were not dysregulated by pollution, and the seven genes that were present and differentially expressed in liver and gill were mainly downregulated. Three CYP genes associated with exogenous compounds showed differential expression in the gill, while four CYP genes associated with endogenous compounds showed differential expression in the liver. The findings presented here highlight the importance of CYP genes, his family, tissues and his interaction in the context of pollution biomarkers use.

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Determination of Soluble Salts in Interstitial Water of Fluvial Sediments by Ie-HPLC

Cited by 3 publications

References 6 publications

Integration of 3D printed Mg²⁺ potentiometric sensors into microfluidic devices for bioanalysis

Integration of 3D printed Mg²⁺ potentiometric sensors into microfluidic devices for bioanalysis

Effects of Different Aquafeed Sources on Growth Performance, Oxidative Capacity, and Fatty Acid Profile of Three Carps Reared in the Semi-Intensive Composite Culture System

Biomarker selection depends on gene function and organ: the case of the cytochrome P450 family genes in freshwater fish exposed to chronic pollution

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Determination of Soluble Salts in Interstitial Water of Fluvial Sediments by Ie-HPLC

Cited by 3 publications

References 6 publications

Integration of 3D printed Mg2+ potentiometric sensors into microfluidic devices for bioanalysis

Integration of 3D printed Mg2+ potentiometric sensors into microfluidic devices for bioanalysis

Effects of Different Aquafeed Sources on Growth Performance, Oxidative Capacity, and Fatty Acid Profile of Three Carps Reared in the Semi-Intensive Composite Culture System

Biomarker selection depends on gene function and organ: the case of the cytochrome P450 family genes in freshwater fish exposed to chronic pollution

Contact Info

Product

Resources

About

Integration of 3D printed Mg²⁺ potentiometric sensors into microfluidic devices for bioanalysis

Integration of 3D printed Mg²⁺ potentiometric sensors into microfluidic devices for bioanalysis