Determination of soluble sugar profile in rice

Hu, Xianqiao; Fang, Changyun; Lü, Lin; Hu, Zhanqiang; Shao, Yiming; Zhu, Zhiwei

doi:10.1016/j.jchromb.2017.05.001

Cited by 31 publications

(14 citation statements)

References 20 publications

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“…As far as our knowledge, no studies have been stated on the free sugar profile of hydrothermally treated non-tradition flakes with coloured layers. Nevertheless, Hu et al (2017) provided an approximate maltose content in milled rice grains up to 1.34 mg/g, fructose content up to 0.11 mg/g, and glucose content up to 2.29 mg/g [16]. Concentrations of sucrose, glucose, and fructose in quinoa seeds were the following: Up to 15.2, 8.0, and 1.6 mg/g, respectively [23].…”

Section: Resultsmentioning

confidence: 99%

“…For the determination of free sugars the milled samples (5 g of each) were extracted with 40 mL of redistilled water (30 °C) by stirring on the magnetic stirrer for 30 min. Then, the volumetric flask (50 mL) was filled up to mark with redistilled water and the sample mixture was filtered through KA 4 of filter paper [16]. Individual sugars were determined using the HPLC system (Dionex Ultimate 3000, Thermo Scientific; Waltham, MA, USA) coupled to a detector (ERC RefractoMax 520, Thermo Scientific; Waltham, MA, USA).…”

Section: Methodsmentioning

confidence: 99%

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The Nutritional Value of Non-Traditional Gluten-Free Flakes and Their Antioxidant Activity

et al. 2019

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Nowadays, there is a growing interest for foods with a lower sugar content and rich in fiber and biologically active substances. The main purpose of this study was to prepare flakes from non-traditional pigmented cereals (Oryza sativa, Chenopodium quinoa, and Eragrostis tef) and to analyze their fibre, sugar, and in vitro digestibility values. Regarding phenolic antioxidants (soluble, soluble conjugated, and insoluble bound fractions), their content and antioxidant activity were measured using spectrophotometry and high performance liquid chromatography (HPLC) methods. Hydrothermally treated grains resulted in flakes with higher total dietary fibre contents (11.1–24.4%), quinoa and teff flakes were rich in maltose (up to 42.0 mg/g). Non-traditional flakes had lower in vitro digestibility, but conversely, they exhibited the highest phenolic contents corresponding with the highest antioxidant activity values (up to 2.33 mg Gallic acid equivalent/g of total phenolic content and 1.59 mg Trolox equivalent/g for 2,2-diphenyl-1-picrylhydrazyl (DPPH) in case of brown teff). Among free phenolics, the main contributors to an antioxidant activity were p-coumaric, o-coumaric, and gallic acids (r > 0.8186); among the soluble conjugated fractions, they were epigallocatechin, epicatechin, caffeic, and vanillic acids (r > 0.5935); while caffeic, protocatechuic, and ferulic acids (r > 0.5751) were the main contributors among the insoluble bound phenolics.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

The Nutritional Value of Non-Traditional Gluten-Free Flakes and Their Antioxidant Activity

et al. 2019

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“…The content of MDA and proline in lettuce seedlings of each treatment was determined by the MDA (malondialdehyde, MDA, Content Assay Kit, Colorimetric Method) and Pro (proline, PRO, Content Assay Kit, Colorimetric Method) content kit produced by Shanghai Sangon Biological Engineering Company. The contents of soluble sugar and soluble protein was assessed by the anthrone colorimetry method and the Coomassie Brilliant Blue method …”

Section: Methodsmentioning

confidence: 99%

Salvia miltiorrhiza Derived Carbon Dots and Their Heat Stress Tolerance of Italian Lettuce by Promoting Growth and Enhancing Antioxidant Enzyme Activity

Wang

Kang

et al. 2021

ACS Omega

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With global warming, plants often suffer damage from high temperatures during the growth process, which inhibits their growth. In this work, carbon dots (CDs), synthesized by Salvia miltiorrhiza (S. miltiorrhiza) with a one-step hydrothermal method, were selected as heat-resistant enhancement agents for plants. Inspired by this background, this work studied Italian lettuce grown at 25, 35, and 45 °C and treated with CD and deionized water control (sprayed on leaves). The results showed that the biomass, chlorophyll content, net photosynthetic rate, activities of SOD (superoxide dismutase), POD (peroxidase), CAT (catalase), soluble sugar, and soluble protein contents of lettuce treated by CDs were increased while the contents of malondialdehyde (MDA) and proline (Pro) were decreased at 35 and 45 °C. The application of CDs at 35 and 45 °C could maintain the growth of plants by reducing oxidative damage and lipid peroxidation especially at the temperature of 35 °C, the growth status of lettuce treated by CDs was no different from that of lettuce grown naturally at the optimal temperature of 25 °C, or even better than the latter. This finding verified that the CDs could significantly improve the high-temperature tolerance of lettuce, thus alleviating the heat stress of plants.

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“…Root length and root weight were measured by a standard ruler and 1/1000 balance; if 60% of the length of the root appeared blackish or brownish, it was calculated as a black root, and the rest were calculated as white roots; the roots shorter than 1 cm in length or decomposed were not calculated in root length and root weight. Root bleeding was collected by the cotton traps method [16], SS was performed according to Hu et al [17], GOT activity was measured through the method of Sax et al [18], and GPT and GS activity was tested via the methods of Zhong et al [19] and Yu and Zhang [20], respectively.…”

Section: Samples and Measurementmentioning

confidence: 99%

Negative and Positive Impacts of Rape Straw Returning on the Roots Growth of Hybrid Rice in the Sichuan Basin Area

et al. 2019

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Incorporating oilseed-rape straw in soil is one of the effective methods for enhancing the use efficiency of agricultural resources in the rape-rice rotation system. However, the impacts of oilseed-rape straw incorporation on root growth and dynamic changes in soil are still unclear. In order to provide a deeper understanding of the oilseed rape straw return on rice growth and productivity, the experiment was conducted in the field and in a specially-designed pots system from 2016 to 2017 by means of two straw returning methods and four straw returning amounts. In the early stage of rice growth (0–36 days after rice transplanting) the straw returning treatments decreased 1.0–8.6 mg/plant in bleeding density and 0.10–6.11, 0.06–0.31, and 0.52–0.84 μmol/(g h) in the activity of glutamine synthetase (GS), glutamic-oxalacetic transaminase (GOT), and glutamic-pyruvic transaminase (GPT), respectively. Oilseed rape straw returned by mulching induced negative impacts on new germinating roots in 0–10 cm of soil, while the negative impacts were observed on roots in 10–30 cm of soil for straw returned by plowing. In the later stage of rice growth (56–75 days after rice transplanting), oilseed rape straw returning produced some positive impacts on rice roots, which enhanced the yield of rice. Conclusively, our findings suggested that dynamic root growth and the activity of root enzymes are two major factors behind the slow reviving of rice after transplanting in the straw returning field. Plowing is a more appropriate method of straw returning than mulching in the rape-rice rotation system in the Sichuan basin area, with a straw incorporation rate of 3.0 t/hm2.

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Determination of soluble sugar profile in rice

Cited by 31 publications

References 20 publications

The Nutritional Value of Non-Traditional Gluten-Free Flakes and Their Antioxidant Activity

The Nutritional Value of Non-Traditional Gluten-Free Flakes and Their Antioxidant Activity

Salvia miltiorrhiza Derived Carbon Dots and Their Heat Stress Tolerance of Italian Lettuce by Promoting Growth and Enhancing Antioxidant Enzyme Activity

Negative and Positive Impacts of Rape Straw Returning on the Roots Growth of Hybrid Rice in the Sichuan Basin Area

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