2021
DOI: 10.1002/cche.10447
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Determination of soluble wheat protein fractions using the Bradford assay

Abstract: Background and objectives Determination of different grain protein fractions in wheat cultivars is an important task in analyzing bread baking quality. In many laboratories, the Bradford assay is used to determine protein concentrations in solutions. In any protein assay (including Bradford), the ideal protein to use as a standard is the purified protein being assayed. In the absence of such an absolute reference, protein another protein must be selected as a relative standard such as bovine serum albumin (BSA… Show more

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Cited by 31 publications
(13 citation statements)
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“…The concentration of the proteins from the extracted fractions was determined spectrophotometrically (Specord ® 50 Plus, Analytik Jena, Jena, Germany) according to the Bradford method by using wheat grain gliadin and glutenin as a reference for calibration [23]. All samples were measured in duplicate to reduce technical errors.…”
Section: Quantitative Analysis Of the Protein Fractionsmentioning
confidence: 99%
“…The concentration of the proteins from the extracted fractions was determined spectrophotometrically (Specord ® 50 Plus, Analytik Jena, Jena, Germany) according to the Bradford method by using wheat grain gliadin and glutenin as a reference for calibration [23]. All samples were measured in duplicate to reduce technical errors.…”
Section: Quantitative Analysis Of the Protein Fractionsmentioning
confidence: 99%
“…Reactions catalyzed by aminotransferases are reversible. Alanine and aspartate may also be involved in the replenishment of the glutamate pool (18) . The important role of the studied aminotransferases in nitrogen mobilization is indisputable.…”
Section: Resultsmentioning
confidence: 99%
“…The method of Tambussi et al (2005) (17) was used in the determination of relative water content at the solar time in threehour intervals on the 13th day of ontogenesis. Protein was measured by the method of Rekowski (2021), using bovine serum albumin as the standard (18) .…”
Section: Methodsmentioning
confidence: 99%
“…The maximal reaction rate ( k cat ) and the Michaelis constant ( K M ) of the purified HS -NRK were calculated using the Michaelis–Menten equation . The protein concentration was quantified using the standard Bradford assay …”
Section: Methodsmentioning
confidence: 99%