Determination of some acidic herbicides by thin-layer chromatography

Guardigli, Alvaro; Chow, W.M.L.; Lefar, Morton S.

doi:10.1021/jf60178a022

Cited by 13 publications

(5 citation statements)

References 3 publications

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“…The TLC separation of herbicides, 4‐(2,4‐DB), 2,4‐D, and 2,4,5‐T, was adapted from Guardigli et al,25 using glass‐backed normal‐phase silica gel plates with organic binder and UV‐254 indicator (HPTLC‐HLF, 150 µm phase, P/N 59077, AnalTech Inc.). The herbicide mix (10000 µg/mL of each herbicide) was spotted as a band (a closely spaced series of 1 µL aliquots) and the plate developed in 85:15 (v/v) toluene/glacial acetic acid.…”

Section: Methodsmentioning

confidence: 99%

Thin‐layer chromatography and mass spectrometry coupled using proximal probe thermal desorption with electrospray or atmospheric pressure chemical ionization

Ovchinnikova

Berkel

2010

Rapid Comm Mass Spectrometry

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An atmospheric pressure proximal probe thermal desorption sampling method coupled with secondary ionization by electrospray or atmospheric pressure chemical ionization was demonstrated for the mass spectrometric analysis of a diverse set of compounds (dyestuffs, pharmaceuticals, explosives and pesticides) separated on various high-performance thin-layer chromatography plates. Line scans along or through development lanes on the plates were carried out by moving the plate relative to a stationary heated probe positioned close to or just touching the stationary phase surface. Vapors of the compounds thermally desorbed from the surface were drawn into the ionization region of a combined electrospray ionization/atmospheric pressure chemical ionization source where they merged with reagent ions and/or charged droplets from a corona discharge or an electrospray emitter and were ionized. The ionized components were then drawn through the atmospheric pressure sampling orifice into the vacuum region of a triple quadrupole mass spectrometer and detected using full scan, single ion monitoring, or selected reaction monitoring mode. Studies of variable parameters and performance metrics including the proximal probe temperature, gas flow rate into the ionization region, surface scan speed, read-out resolution, detection limits, and surface type are discussed.

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Section: Methodsmentioning

confidence: 99%

Thin‐layer chromatography and mass spectrometry coupled using proximal probe thermal desorption with electrospray or atmospheric pressure chemical ionization

Ovchinnikova

Berkel

2010

Rapid Comm Mass Spectrometry

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“…The 2,4-D-containing samples and MSD liquid medium were adjusted to pH 2.0 with 6 N H 2 SO 4 (Feung et al 1978;Guardigli et al 1971). An equivalent volume of diethyl ether was added to the medium, which was then mixed, and the diethyl ether layer collected.…”

mentioning

confidence: 99%

“…The medium with added 2,4-D was incubated by shaking (100 rpm) at 25°C in darkness. The diethyl ether extracted from the incubated medium was analyzed by TLC using benzene-acetic acid (75 : 12, v/v) (Guardigli et al 1971). A marked decrease in 2,4-D was observed in the diethyl ether extracted from the media incubated for both 10 and 20 days ( Figure 4A).…”

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confidence: 99%

Anise-cultured cells abolish 2,4-dichlorophenoxyacetic acid in culture medium

Tanida

Shiota

2019

Plant Biotechnology

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In anise (Pimpinella anisum, family Apiaceae), callus-like embryogenic cells (embryogenic callus) are induced by culturing hypocotyl explants in 2,4-dichlorophenoxyacetic acid (2,4-D)-containing medium, and somatic embryos are formed from embryogenic callus transferred into 2,4-D-free medium. Anise somatic embryos are also induced even if embryogenic callus is continually cultured in 2,4-D-containing medium without subculturing. In this study, we aimed to clarify the dynamics of 2,4-D during anise cell culture. After culturing anise callus in 2,4-D-containing medium, 2,4-D in the medium was analyzed by thin-layer chromatography. In the medium, 2,4-D was decreased during anise callus culture, and fully abolished after 5-day culture. On the other hand, no decrease in 2,4-D was observed in the other Apiaceae species (carrot, fennel, dill, parsley, and coriander). After 7-day culture of anise callus, the medium was collected following removal of the cultured cells and 2,4-D was added to the collected medium. After 10 days of incubation and shaking, 2,4-D was markedly decreased in the medium. However, when the collected medium was heat-treated at 100°C, 2,4-D was detected after 20 days of incubation. Therefore, anise callus has a specific 2,4-D degradation system, in which heat-inactivated secreted molecules may participate.

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“…McKellar (1971) reported a method for dinoseb determination in milk and cream. Guardigli et al (1971) developed a TLC procedure for dinoseb residues. Dekker and Selling (1975) in the Netherlands presented a method for dinoterb (2-tert-butyl-4,6-dinitrophenol) in soil.…”

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confidence: 99%

A method to determine dinoseb residues in crops and soil by gas chromatography

Gardner¹,

McKellar²

1980

J. Agric. Food Chem.

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A method is described for the determination of residues of dinoseb (2-sec-butyl-4,6-dinitrophenol) in alfalfa, corn, cottonseed, field beans, almonds, peanuts, peas, potatoes, soybeans, grapes, oranges, peaches, pears, barley, wheat, and soil at levels ranging from 0.05 to 100 ppm. Dinoseb is first extracted by hot hydrolysis in methanol-sulfuric acid and subsequently partitioned into diethyl ether and adsorbed onto basic alumina. After elution with sodium bicarbonate, ether partition, and diazomethane methylation, the dinoseb methyl ether is adsorbed onto acidic alumina and eluted with ether. Electron-capture gas chromatography provides a sensitive means of quantifying residues of dinoseb down to 20 pg. Average recoveries ranged from 77 to 99%.

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Determination of some acidic herbicides by thin-layer chromatography

Cited by 13 publications

References 3 publications

Thin‐layer chromatography and mass spectrometry coupled using proximal probe thermal desorption with electrospray or atmospheric pressure chemical ionization

Thin‐layer chromatography and mass spectrometry coupled using proximal probe thermal desorption with electrospray or atmospheric pressure chemical ionization

Anise-cultured cells abolish 2,4-dichlorophenoxyacetic acid in culture medium

A method to determine dinoseb residues in crops and soil by gas chromatography

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