Determination of some cardiac glycosides by high-performance liquid chromatography

Şener, Bilge; Evren, Namık; Özgüven, Menşure

doi:10.1016/0378-8741(88)90091-8

Cited by 2 publications

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“…Goat pea (Securigera securidaca) is one of the winter legume plants that grows in moderately moist environments around the world, and its use in many pharmaceutical industries and for the treatment of a number of diseases, including high blood pressure, diabetes, acute infections and the intestinal system [1,2], and intestinal ulcers and cancerous tumors [3,4], as well as its use in the treatment of some heart diseases [5]. As for the seeds, they are rich in cardiac glycosides, which are attributed to their vital effect in inhibiting blood sugar and as antioxidant compounds [6].…”

Section: Introductionmentioning

confidence: 99%

The Effect of Spraying with the Hormone Brassinolide on the Production of Oil and Glycosides of Goat Pea (Securigera securidaca)

Hassan

2023

IOP Conf. Ser.: Earth Environ. Sci.

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A field experiment was carried out in Diyala Governorate during the winter season 2020-2021 to evaluate the effect of spraying three concentrations of Brassinolide hormone (1, 2 and 3 ppm) in addition to the control treatment (0 ppm) and three dates of spraying (after 20,40 and 60 days of germination) in the production of oil and total glycosides in Securigera securidaca seeds, by using a factorial experiment according to the randomized complete block design. The results showed that the spraying treatment with a concentration of 2 ppm was superior in seed yield, content and yield of oil, and content of total glycosides in the seeds, which reached 9.76 g/plant, 4.00%, 15.43 kg/ha and 1.770 mg/100 g seeds respectively, while the second date of spraying with the hormone Brassinolide was superior in seed yield, content and yield of oil, and total glycosides, reached 9.39 g/plant, 3.76%, 13.15 kg/ha and 1.750 mg/100 g seeds respectively. The results also show that the interaction treatment was superior at concentration 2ppm in the second date and gave the highest content and yield of oil and total glycosides which reached 4.24%, 17.54 kg/ha and 1.820 mg/100 g seeds respectively.

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Section: Introductionmentioning

confidence: 99%

The Effect of Spraying with the Hormone Brassinolide on the Production of Oil and Glycosides of Goat Pea (Securigera securidaca)

Hassan

2023

IOP Conf. Ser.: Earth Environ. Sci.

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Applicability of high-performance liquid chromatography to the analysis of cardiac glycosides in lily-of-the-valley medicines (review)

Beketova,

Evdokimova,

Shemeryankina

et al. 2024

Žurnal prikladnoj himii

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Introduction. The standardisation of lily-of-the-valley (Convallaria majalis) herbal drugs containing cardiac glycosides of the cardenolide group currently uses either animal bioassays or spectrophotometry. The need to explore the possibility of abandoning animal testing makes it essential to find a selective and sensitive physicochemical method for the determination of cardiac glycosides in lily of the valley. Aim. This study aimed to select an appropriate alternative to animal testing through a comparative analysis of scientific publications on physicochemical methods for the determination of cardenolide cardiac glycosides. Discussion. According to the analysis of identification and quantification methods for cardenolides, animal testing does not provide sufficient accuracy and reproducibility for the standardisation of herbal drugs, herbal drug preparations, and herbal medicinal products. However, spectrophotometry does not provide sufficient specificity, and thin-layer chromatography is mainly applicable to the identification of cardenolides. This article presents the results of comparing the chromatographic conditions used for cardenolide determination by high-performance liquid chromatography (HPLC) with various detectors. According to the study results, HPLC can replace animal bioassays or insufficiently selective methods in the determination of cardiac glycosides in lily-of-the-valley products. The development of analytical procedures for cardenolide determination can be based upon reverse-phase HPLC with octadecylsilyl (С18) as the stationary phase, a solvent mixture comprising water, acetonitrile, and/or methanol in different proportions as the mobile phase, and a diode-array detector at 220 ± 2 nm. Conclusions. The results of this comparative analysis indicate that the method for the identification and quantification of cardiac glycosides in lily-of-the-valley herbal drugs, herbal drug preparations, and herbal medicinal products is promising for further study and experimentation. HPLC is applicable for routine use and can replace animal bioassays or insufficiently selective methods in the determination of lily-of-the-valley cardiac glycosides.

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Determination of some cardiac glycosides by high-performance liquid chromatography

Cited by 2 publications

References 0 publications

The Effect of Spraying with the Hormone Brassinolide on the Production of Oil and Glycosides of Goat Pea (Securigera securidaca)

The Effect of Spraying with the Hormone Brassinolide on the Production of Oil and Glycosides of Goat Pea (Securigera securidaca)

Applicability of high-performance liquid chromatography to the analysis of cardiac glycosides in lily-of-the-valley medicines (review)

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