Determination of Specific and Non-Specific Parameters of the Simplicia and Ethanol Extract of Sangketan (Achyranthes Aspera L.) Leaves

Abstract

Objective: With recent advancements in traditional medicine, there is an increasing need for quality assurance assessments using standardizedprocesses. In this study, we aimed to determine the specific and non-specific parameters of the Simplicia and 70% ethanol extract of Achyranthesaspera leaves from three different regions.Methods: Samples were extracted using a maceration method with 70% ethanol as the solvent. The tests for the specific and non-specific parameterswere based on the Herbal Pharmacopoeia of Indonesia.Results: Our results of the specific parameters of the Simplicia showed that the ethanol-soluble extract contained 5.83–9.36% and the water-solubleextract contained 10.25–15.44%. The chromatogram pattern used β-sitosterol as the standard, and the total phenolic content was 0.93–1.15 mg gallicacid equivalent (GAE)/g Simplicia. The results of the non-specific parameters of Simplicia showed a loss on drying of 15.25–15.91%, a total ash contentof 14.58–20.79%, and an acid-insoluble ash value of 1.75–2.19%.Conclusion: Our results of the specific parameters of A. aspera extract showed a total phenolic content of 6.49–7.68% mg GAE/g extract. Our resultsof the non-specific parameters of the extract showed a total ash value of 13.18–14.52%, an acid-insoluble ash value of 0.14–0.29%, and a moisturecontent of 10.42–11.16%.

Sub-Acute Toxicity Study of Pegagan Embun (Hydrocotyle Sibthorpioides Lam.) Extract on the SGPT and Sgot Level of Wistar White Male Rats

“…Particular parameters were as follows: organoleptic, phytochemical screening, thin layer chromatography (TLC) profile, and total flavonoid content. The following nonspecific characteristics were used: acidinsoluble ash content, total ash content, and loss on drying [15].…”